Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 608,245 C→T 7.5% A39V (GCC→GTC)  PP_0524 → cobalamin‑binding protein HutB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947608,2450CT 7.5% 151.9 / 2.5 53A39V (GCC→GTC) PP_0524cobalamin‑binding protein HutB
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base T (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

GTACCTCTATCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGG  >  NC_002947/608097‑608386
                                                                                                                                                    |                                                                                                                                             
gTACCTCTATCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGc                                                                                                                                               >  3:304414/1‑149 (MQ=255)
 tACCTCTATCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGcc                                                                                                                                              >  4:275668/1‑149 (MQ=255)
   ccTCTATCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCcga                                                                                                                                            >  7:25667/1‑149 (MQ=255)
    ctctATCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCcgac                                                                                                                                           <  8:25667/149‑1 (MQ=255)
    ctctATCGCATTCCGCGCCAGATGGGTTGATGCGACTGCTAACTGGCCTGCTGGCGTTGTTAGCCTGAACAGTAATGGCTGCAGAACCGCTAAGTGTCGTCAGTATGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCagac                                                                                                                                           >  5:347932/1‑149 (MQ=255)
         tCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACct                                                                                                                                      >  5:180127/1‑149 (MQ=255)
           gCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACctgc                                                                                                                                    >  8:114871/1‑149 (MQ=255)
            cATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGTCGTCGGCctg                                                                                                                                     >  4:234891/1‑147 (MQ=255)
            cATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGAGGACctgct                                                                                                                                   >  8:212639/1‑149 (MQ=255)
            cATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACctgct                                                                                                                                   <  7:19244/149‑1 (MQ=255)
            cATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACAGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACctgct                                                                                                                                   >  7:56488/1‑149 (MQ=255)
              ttCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGTCGTCGGCctgcagc                                                                                                                                 <  3:234891/149‑4 (MQ=255)
                ccGCGCCAGCGGGGTTGATGCGACTGCTACATGGCCTTCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCtggt                                                                                                                                <  8:56488/148‑1 (MQ=255)
                  gcgcCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTggg                                                                                                                             >  5:179721/1‑149 (MQ=255)
                   cgcCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGc                                                                                                                            <  6:302532/149‑1 (MQ=255)
                     ccAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGt                                                                                                                          <  5:182542/149‑1 (MQ=255)
                             gtggATGCGACTGCTACCTGGCCTGCTGGCGGTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCGGGTGGGCGTGCTCGATg                                                                                                                  <  4:278578/146‑1 (MQ=255)
                              ttGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATgg                                                                                                                 >  3:137184/1‑149 (MQ=255)
                                 aTGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATggcgg                                                                                                              <  3:275668/149‑1 (MQ=255)
                                 aTGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATggcgg                                                                                                              <  4:304414/149‑1 (MQ=255)
                                  tGCGACTGCTAACTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCTAGATCATGCTTTAGCTGCAGGCCGACTACCTGCTGGTGTGCGTGCTCGATggcggc                                                                                                             <  6:347932/149‑1 (MQ=255)
                                            aCCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAAcggccgg                                                                                                   >  8:115143/1‑149 (MQ=255)
                                                 gCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGc                                                                                               <  6:179721/148‑1 (MQ=255)
                                                                  gCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTtc                                                                             <  4:137184/149‑1 (MQ=255)
                                                                     tGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTtcggt                                                                          >  1:216590/1‑149 (MQ=255)
                                                                       cACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTtcggtc                                                                         <  7:107943/148‑1 (MQ=255)
                                                                         ccGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCggg                                                                      <  8:195254/149‑1 (MQ=255)
                                                                          cGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGgc                                                                     >  4:320215/1‑149 (MQ=255)
                                                                             aCTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCggg                                                                      >  3:203539/1‑145 (MQ=255)
                                                                             aCTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCggg                                                                      <  4:203539/145‑1 (MQ=255)
                                                                                 gCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGTCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAgg                                                              <  5:73233/149‑1 (MQ=255)
                                                                                       gAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACt                                                        >  5:238836/1‑149 (MQ=255)
                                                                                         aCCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTg                                                       >  7:72588/1‑148 (MQ=255)
                                                                                             cTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACAt                                                  >  5:280612/1‑149 (MQ=255)
                                                                                                         aGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGc                                       <  6:280612/148‑1 (MQ=255)
                                                                                                          gTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTc                                     >  2:277739/1‑149 (MQ=255)
                                                                                                           tCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCa                                    <  6:238836/149‑1 (MQ=255)
                                                                                                               gCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCt                                >  1:39978/1‑149 (MQ=255)
                                                                                                                 ccccTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTgc                              <  3:273938/149‑1 (MQ=255)
                                                                                                                     tCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGccc                          >  1:324540/1‑149 (MQ=255)
                                                                                                                       gATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGccc                          <  3:320215/147‑1 (MQ=255)
                                                                                                                            gCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATAGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTgcg                             >  6:125148/1‑139 (MQ=255)
                                                                                                                            gCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATAGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTgcg                             <  5:125148/139‑1 (MQ=255)
                                                                                                                             cGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGACGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGt                  >  5:330883/1‑149 (MQ=255)
                                                                                                                               agaTCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTtg                <  6:212589/149‑1 (MQ=255)
                                                                                                                                 aTCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTtgct              <  7:212639/149‑1 (MQ=255)
                                                                                                                                   cATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGAc                      <  3:107508/139‑1 (MQ=255)
                                                                                                                                   cATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGAc                      >  4:107508/1‑139 (MQ=255)
                                                                                                                                       cTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAAcggccggc                                                                                                  >  2:189624/1‑59 (MQ=255)
                                                                                                                                       cTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAAcggccggc                                                                                                  <  1:189624/59‑1 (MQ=255)
                                                                                                                                       cTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCtgt         <  1:228537/148‑1 (MQ=255)
                                                                                                                                           aGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGcc     <  7:115143/148‑1 (MQ=255)
                                                                                                                                             cagCAGGTCGTCGGCCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAgg  >  7:278360/3‑149 (MQ=255)
                                                                                                                                                cAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCtgtg        >  4:257076/1‑140 (MQ=255)
                                                                                                                                                cAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCtgtg        <  3:257076/140‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GTACCTCTATCGCATTCCGCGCCAGCTGGGTTGATGCGACTGCTACCTGGCCTGCTGGCGTTGTTCGCCTGCACCGTACTGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGG  >  NC_002947/608097‑608386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑