| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 644,141 | G→C | 9.0% | A292A (GCC→GCG) | acoA ← | acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 644,141 | 0 | G | C | 9.0% | 237.2 / 10.4 | 78 | A292A (GCC→GCG) | acoA | acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (36/35); new base C (4/3); total (40/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAAATGG > NC_002947/643998‑644285 | cttGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCATCGACGCCCGCGt < 7:205282/148‑1 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATg < 3:103302/149‑1 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCGCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGGCTTCGATCAGGTCCTCGACGCGGGCGTCGAt < 2:287911/148‑1 (MQ=255) cATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATgg > 1:195488/1‑149 (MQ=255) cATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATgg > 3:92407/1‑149 (MQ=255) aTGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGc < 3:244496/149‑1 (MQ=255) gtggtTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCgg < 6:54561/135‑1 (MQ=255) gtggtTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCgg > 5:54561/1‑135 (MQ=255) tggtTCTCCTGTTGTTCTTGTATTCCGCGGCTCTGGGGTTGGCGACGTAGAGGTCGGTGAGAAGGTCGGCGGGCTGTGGCTTGGGTTCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGAGtg > 5:326225/1‑147 (MQ=255) ctcCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGtt < 3:282258/148‑1 (MQ=255) tcCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCTATCAGGTCCTCGACGCGGGCGTCGATGGCGTCAAGTTGc > 3:47396/1‑149 (MQ=255) cTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTc > 4:245065/1‑149 (MQ=255) cTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTc < 3:303514/149‑1 (MQ=255) tgttgtTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCt > 3:299860/1‑147 (MQ=255) tgttgtTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCt < 4:299860/147‑1 (MQ=255) tgttgtTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCg > 2:24995/1‑149 (MQ=255) tgttgtTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCAGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCg > 7:225363/1‑149 (MQ=255) ttgttCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGc < 3:245065/149‑1 (MQ=255) ttgttCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGagga > 7:58946/1‑130 (MQ=255) ttgttCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGagga < 8:58946/133‑4 (MQ=255) tGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGCTCAGGTCCTCGACGCGGGCGTCGa > 8:6424/1‑123 (MQ=255) agTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCGGTGGCTTGGGAGCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAg > 2:77093/2‑148 (MQ=255) gTAATCCGCGGCTCAGGTGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACAGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGc < 7:241120/148‑1 (MQ=255) tAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCa < 5:8534/148‑1 (MQ=255) ccGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGc > 6:250151/1‑112 (MQ=255) ccGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGc < 5:250151/112‑1 (MQ=255) ccGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGa > 4:315662/1‑141 (MQ=255) ccGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGa < 3:315662/141‑1 (MQ=255) gCTCAGGGGTAGGCGACGGAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCGTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGATGCTCGGCCGTGAGCAGGCCGGcgcg < 4:273441/149‑1 (MQ=255) gggTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATCTTTGGCGCGGCGACTTAAGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCgg < 1:107264/148‑1 (MQ=255) gTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCt > 7:235643/1‑149 (MQ=255) accGACGTCTACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCttgt > 5:197025/3‑149 (MQ=255) accGACGTCTACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCttgt > 6:29824/3‑149 (MQ=255) gCGACGTAGACCTCGGTGAGCAGGTCGGCGGGCGGTGGCTTGGGAGCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGGCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGGGAGCAGGCCGGCGCGGGTGGTCttgtt < 8:323952/149‑1 (MQ=255) cGACGTCTACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCttgttg < 6:197025/149‑1 (MQ=255) gACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGc > 1:237000/1‑149 (MQ=255) aGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGc < 1:344167/88‑1 (MQ=255) aGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGc > 2:344167/1‑88 (MQ=255) aGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGc < 8:325675/107‑1 (MQ=255) cGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGc < 8:197001/149‑1 (MQ=255) gTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCa > 3:71643/1‑149 (MQ=255) cGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGAGCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATg < 4:22070/149‑1 (MQ=255) gTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTcgc > 8:40538/1‑149 (MQ=255) gTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTcgc < 1:60701/149‑1 (MQ=255) gTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTcgc > 1:17571/1‑149 (MQ=255) ggCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTAGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAg < 4:92407/149‑1 (MQ=255) cTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGt > 7:116814/1‑149 (MQ=255) cTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGt > 7:22947/1‑149 (MQ=255) gggATCGGA‑‑TTTGGCGCGGCTA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttc < 2:237000/148‑1 (MQ=255) ggATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttctt < 5:29824/149‑1 (MQ=255) aTCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGGGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttctt < 2:329961/147‑1 (MQ=255) aTCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGGGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttctt > 1:329961/1‑147 (MQ=255) gA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTcttcac > 1:300131/1‑146 (MQ=255) gA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTcttcac < 2:300131/146‑1 (MQ=255) a‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttctt > 2:57391/1‑142 (MQ=255) a‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttctt < 1:57391/142‑1 (MQ=255) tttGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTtcat > 3:275611/1‑149 (MQ=255) ttGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTcgc < 4:12024/130‑1 (MQ=255) ttGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTcgc > 3:12024/1‑130 (MQ=255) ggCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTtcatca < 8:235643/148‑1 (MQ=255) gcggcgA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAgg > 4:262732/1‑130 (MQ=255) gcggcgA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAgg < 3:262732/130‑1 (MQ=255) gcggcgA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAgggg > 2:61864/1‑149 (MQ=255) a‑‑aCGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGcgcg > 2:144430/1‑147 (MQ=255) a‑‑aCGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGcgcg < 1:144430/147‑1 (MQ=255) cGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTgc < 1:24995/146‑1 (MQ=255) cGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTgc < 2:17571/146‑1 (MQ=255) cGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCa > 5:205414/1‑149 (MQ=255) ggTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCt > 4:296960/1‑149 (MQ=255) ggTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCt > 2:120459/1‑149 (MQ=255) tCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTc < 8:116814/149‑1 (MQ=255) ccTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTc > 3:275669/1‑148 (MQ=255) ccTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTc < 4:275669/148‑1 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTg < 5:312056/134‑1 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTg > 6:312056/3‑136 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTc > 1:6660/3‑148 (MQ=255) caacGACGCCCGCGTCGACGGCGTCCAGTTGCTCGGCCGTGAGCACGCCGGCGCCGGGGGTCTTGTAGCGAAAAAGCATGAGGCACTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGCCGCGGTAGATTTGCGCATCGCCGTc > 4:246419/4‑148 (MQ=255) tCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGa > 5:187823/1‑148 (MQ=255) gACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAAAt > 8:287498/1‑149 (MQ=255) cgcgGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAAATgg < 8:22947/149‑1 (MQ=255) | CTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGA‑‑TTTGGCGCGGCGA‑‑ACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAAATGG > NC_002947/643998‑644285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |