Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 646,272 T→A 10.2% intergenic (‑178/‑106) PP_0556 ← / → acoR acetoin catabolism protein/acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947646,2720TA10.2% 163.5 / 8.5 59intergenic (‑178/‑106)PP_0556/acoRacetoin catabolism protein/acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base A (3/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

CAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTCAGCA  >  NC_002947/646130‑646420
                                                                                                                                              |                                                                                                                                                    
cAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCGCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGtct                                                                                                                                                <  2:98344/149‑1 (MQ=255)
   gCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCaa                                                                                                                                             <  7:128741/149‑1 (MQ=255)
    cGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCAGCGTCTCagt                                                                                                                                            <  6:190999/149‑3 (MQ=255)
           ccAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                     >  1:311743/1‑149 (MQ=255)
           ccAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                     >  4:107678/1‑149 (MQ=255)
           ccAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                     >  3:183997/1‑149 (MQ=255)
                 ttcttcGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAAt                                                                                                                                >  8:100718/1‑148 (MQ=255)
                  tcttcGCGCTGGCGGCAGTGGCCGGGCTTGCTGGCTCTGGTCTTGTTCGGGCTGGTTTTCCCGGGTAGGGTGGCGTGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATc                                                                                                                               <  2:308827/148‑1 (MQ=255)
                    ttcGCGATGGCTGAAGTCGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCTGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCACGCCAGCCGGTAATcaca                                                                                                                            >  4:98926/1‑149 (MQ=255)
                     tcGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCAGCGTCTCag                                                                                                                                             <  4:167240/131‑2 (MQ=255)
                     tcGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCAGCGTCTCag                                                                                                                                             >  3:167240/1‑130 (MQ=255)
                      cgcgATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATcaca                                                                                                                            <  6:315862/147‑1 (MQ=255)
                                  aGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCggg                                                                                                              <  7:122973/149‑1 (MQ=255)
                                      gCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCAt                                                                                                          <  2:311743/149‑1 (MQ=255)
                                            cTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGCGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATAcc                                                                                                       >  5:71657/1‑146 (MQ=255)
                                            cTAGAGGGCTATGGTCGTGTACGGGCAGGTTTTCAATGTTAGGGTGGCGAGACGACGGGTGACAGCGGCGGGCCTGCCTTGCAGAGCCACTGAGACGCTCCGTCTCAAGACAGCCGGTAATCACACAGTGCTATTCGGTGCATGcg                                                                                                       >  8:281612/1‑145 (MQ=255)
                                             tAGCGGGATCTGGTCGTGTGCGGGCAGGTTTTCACGGGTAGGGTGGCGAGTCGCAGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCaga                                                                                                   <  8:319875/149‑1 (MQ=255)
                                                    ctctGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCag                                                                                                    <  3:181769/141‑1 (MQ=255)
                                                    ctctGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCag                                                                                                    >  4:181769/1‑141 (MQ=255)
                                                     tctGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCtt                                                                                            >  1:19076/1‑148 (MQ=255)
                                                        ggTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATg                                                                                        >  3:283640/1‑149 (MQ=255)
                                                         gTCGTGTTCGGGCAGGTTTTCACGGGTGGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATgg                                                                                       >  2:331998/1‑149 (MQ=255)
                                                         gTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAAt                                                                                         <  7:100718/147‑1 (MQ=255)
                                                            gtgtTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGcc                                                                                     <  1:327203/148‑1 (MQ=255)
                                                             tgtTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCgg                                                                                   <  2:19076/149‑1 (MQ=255)
                                                               ttCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGa                                                                                  >  8:34621/1‑148 (MQ=255)
                                                                  gggCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCTTGCACAGAGGCGCTTAATGCCAGGACAAc                                                                              >  2:325856/1‑149 (MQ=255)
                                                                   ggCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACg                                                                             <  7:34621/149‑1 (MQ=255)
                                                                    gCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGa                                                                            >  4:310329/1‑149 (MQ=255)
                                                                     cAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGAt                                                                           <  3:310329/149‑1 (MQ=255)
                                                                        gTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaa                                                                         >  8:91873/1‑148 (MQ=255)
                                                                         ttttCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaaa                                                                        >  5:17835/1‑148 (MQ=255)
                                                                           ttCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaaaaa                                                                      <  1:72982/148‑1 (MQ=255)
                                                                            tCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaaaaa                                                                      >  5:193721/1‑147 (MQ=255)
                                                                             cACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACa                                                                    <  4:183997/148‑1 (MQ=255)
                                                                              aCGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAg                                                                   >  4:2826/1‑148 (MQ=255)
                                                                              aCGGGTAGGGTGGCGAGACGCCGGGCGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATACCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGt                                                                  <  6:71657/149‑1 (MQ=255)
                                                                                       gTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAAc                                                         >  3:120281/1‑149 (MQ=255)
                                                                                             agaCGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAgg                                                   >  6:312470/1‑149 (MQ=255)
                                                                                                 gCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCATGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCt                                               >  6:167130/1‑149 (MQ=255)
                                                                                                  ccGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTg                                              <  3:98926/149‑1 (MQ=255)
                                                                                                   cGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGa                                             <  3:2826/149‑1 (MQ=255)
                                                                                                    gggTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGaa                                            <  6:193721/149‑1 (MQ=255)
                                                                                                     ggTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAAt                                           <  1:331998/149‑1 (MQ=255)
                                                                                                         ccAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCtt                                       <  6:296344/149‑1 (MQ=255)
                                                                                                              gcCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCcgc                                  <  6:17835/149‑1 (MQ=255)
                                                                                                                 gggCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGaa                               <  5:312470/149‑1 (MQ=255)
                                                                                                                      tGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACgag                                                            <  6:293546/115‑1 (MQ=255)
                                                                                                                      tGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACgag                                                            >  5:293546/1‑115 (MQ=255)
                                                                                                                          ttGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGc                      <  4:283640/149‑1 (MQ=255)
                                                                                                                              cGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCAt                  >  4:189941/1‑149 (MQ=255)
                                                                                                                               gagaCACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAAc                                                                     <  7:79433/97‑1 (MQ=255)
                                                                                                                               gagaCACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAAc                                                                     >  8:79433/1‑97 (MQ=255)
                                                                                                                                   gcttGAGACGCAGCGTCTCAAGACAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACAGGAGGGCCTGAAAGCTTGCCGCGAACTCCCGAGCCCATCTGGa             >  8:204415/4‑149 (MQ=255)
                                                                                                                                    cttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCcg                                   <  5:111404/124‑1 (MQ=255)
                                                                                                                                    cttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCcg                                   >  6:111404/3‑126 (MQ=255)
                                                                                                                                    acTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGAc            >  7:267018/1‑149 (MQ=255)
                                                                                                                                       gagaCGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCg                                                       <  5:109116/103‑1 (MQ=255)
                                                                                                                                       gagaCGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCg                                                       >  6:109116/1‑103 (MQ=255)
                                                                                                                                              tGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTcagca  <  8:267018/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTCAGCA  >  NC_002947/646130‑646420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑