Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 657,224 T→A 6.6% V219D (GTC→GAC)  PP_0564 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947657,2240TA 6.6% 175.3 / 2.7 61V219D (GTC→GAC) PP_0564hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/24);  new base A (2/2);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

AAGAGCTGGATCCTGCCATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCATGGCTGCAG  >  NC_002947/657079‑657369
                                                                                                                                                 |                                                                                                                                                 
aaGAGCTGGATCCTGCCATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGc                                                                                                                                                >  6:1410/1‑149 (MQ=255)
        gATCCTGCCATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGTTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACACGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTc                                                                                                                                        >  4:74671/1‑149 (MQ=255)
         aTCCTGCCATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCt                                                                                                                                       <  5:188281/149‑1 (MQ=255)
             tGCCATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGc                                                                                                                                   >  2:292881/1‑149 (MQ=255)
               cgaTCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTc                                                                                                                                 <  8:24549/147‑1 (MQ=255)
               ccATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACATCTGGCTGGGTGACGTCGCCACCCGTCTGGGCTc                                                                                                                                 <  1:11481/149‑1 (MQ=255)
                cATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCa                                                                                                                                              <  2:128480/135‑1 (MQ=255)
                cATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCa                                                                                                                                              >  1:128480/1‑135 (MQ=255)
                             ggAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGcc                                                                                                                   >  3:239329/1‑149 (MQ=255)
                                        agGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGc                                                                                                        <  5:1410/149‑1 (MQ=255)
                                            cATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTg                                                                                                    >  6:176965/1‑149 (MQ=255)
                                             aTCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAACGCTgg                                                                                                   >  5:324203/1‑149 (MQ=255)
                                                  gTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAACGCTGGCCgac                                                                                              <  6:324203/149‑3 (MQ=255)
                                                   tCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCATCGCCTGTCGGCCAACGCTGGCCgaca                                                                                             <  8:53528/149‑4 (MQ=255)
                                                       tGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCAGGGTCAAg                                                                                         >  7:1283/1‑149 (MQ=255)
                                                           ccGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAg                                                                                                         >  1:86286/1‑129 (MQ=255)
                                                           ccGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAg                                                                                                         <  2:86286/129‑1 (MQ=255)
                                                           ccGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTc                                                                                      >  1:116983/1‑148 (MQ=255)
                                                                 ccAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAAcacca                                                                               >  7:261136/1‑149 (MQ=255)
                                                                  cAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGGCGACGTCAcc                                                                                                                                            <  4:332510/87‑1 (MQ=255)
                                                                  cAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGGCGACGTCAcc                                                                                                                                            >  3:332510/1‑87 (MQ=255)
                                                                  cAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAAcaccac                                                                              >  6:219558/1‑149 (MQ=255)
                                                                   aGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACg                                                                             <  7:68554/149‑1 (MQ=255)
                                                                     aCCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCt                                                                           <  5:176965/149‑1 (MQ=255)
                                                                     aCCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCAGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCt                                                                           >  8:216282/1‑149 (MQ=255)
                                                                         gTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTc                                                                                                                                 <  5:224287/91‑1 (MQ=255)
                                                                         gTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTc                                                                                                                                 >  6:224287/1‑91 (MQ=255)
                                                                         gTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTg                                                                          >  8:244059/1‑146 (MQ=255)
                                                                             ggCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCt                                                                                                                              >  5:193124/1‑90 (MQ=255)
                                                                             ggCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCt                                                                                                                              <  6:193124/90‑1 (MQ=255)
                                                                              gCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGAcc                                                                    <  2:116983/147‑1 (MQ=255)
                                                                                tGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAgt                                                                >  1:296454/1‑149 (MQ=255)
                                                                                  ccGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAgt                                                                <  8:261136/147‑1 (MQ=255)
                                                                                       gACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGt                                                         >  3:255316/1‑149 (MQ=255)
                                                                                          aaGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGc                                                      >  8:235922/1‑149 (MQ=255)
                                                                                            gggCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCCACACCACGCTGAAGAACGAGTCGGGCGTTGCCg                                                    >  8:279599/1‑149 (MQ=255)
                                                                                             ggCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCgg                                                   <  4:326493/149‑1 (MQ=255)
                                                                                                gcTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGc                                                  >  7:48593/1‑147 (MQ=255)
                                                                                                  tATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAgg                                              >  7:141032/1‑149 (MQ=255)
                                                                                                      ttctATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGaa                                                                        >  8:222363/1‑119 (MQ=255)
                                                                                                      ttctATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGaa                                                                        <  7:222363/119‑1 (MQ=255)
                                                                                                            aCCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGt                                    <  7:163745/149‑1 (MQ=255)
                                                                                                              ccGCGCCGACAAGCTGAACATCTGGCTGGGTGACGGCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGt                                                         <  6:54559/126‑1 (MQ=255)
                                                                                                              ccGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGAAGCGCCTGTCGGCCAGCGTTGGCCGGGGCAAGCTCAACACCACGCTGCAGCCCGCGTCGGGCGt                                                         >  5:54559/1‑126 (MQ=255)
                                                                                                                gcgcCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGAt                                >  6:180069/1‑149 (MQ=255)
                                                                                                                gcgcCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGAt                                <  4:255316/149‑1 (MQ=255)
                                                                                                                    cGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATgagg                            <  2:296454/149‑1 (MQ=255)
                                                                                                                      aCAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATgag                             <  6:231787/146‑1 (MQ=255)
                                                                                                                      aCAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATgag                             >  5:231787/1‑146 (MQ=255)
                                                                                                                           cTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCtggt                     >  4:275309/1‑149 (MQ=255)
                                                                                                                              aacaacTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCtggtgg                   >  1:256306/1‑148 (MQ=255)
                                                                                                                                      gctggGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAAcccc            <  3:74671/147‑1 (MQ=255)
                                                                                                                                      gctggGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAAcccc            <  5:219558/147‑1 (MQ=255)
                                                                                                                                      gctggGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCAt          >  6:300818/1‑149 (MQ=255)
                                                                                                                                      gctggGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCAt          >  6:210989/1‑149 (MQ=255)
                                                                                                                                      gctggGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCAt          >  3:78031/1‑149 (MQ=255)
                                                                                                                                           gTGGCGACGTCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCg                                                    >  1:192599/1‑102 (MQ=255)
                                                                                                                                           gTGGCGACGTCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCg                                                    <  2:192599/102‑1 (MQ=255)
                                                                                                                                            tGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCATGGCTGc    <  7:244059/149‑1 (MQ=255)
                                                                                                                                              aCGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCATGGCTGCAg  <  7:235922/149‑1 (MQ=255)
                                                                                                                                                gTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACAACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGt                                    >  3:194650/1‑113 (MQ=255)
                                                                                                                                                 tCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCATGGCTg     >  4:186620/1‑143 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAGAGCTGGATCCTGCCATCGAGCGAGTCGGAGTTCGAAGAGGGCATCAAGTCGCTGACCCGCTACCAGACCCGTCTGGCTGCCGGCGACAAGGGCGCTATCTTCTATACCCGCGCCGACAACCTGAACAACTGGCTGGGTGACGTCGCCACCCGTCTGGGCTCGCTGTCGCAGCGCCTGTCGGCCAGCGTTGGCCGGGTCAAGCTCAACACCACGCTGAAGACCGAGTCGGTCGTTGCCGGCCAGGCGCCGCAGGTGGATGAGGAGCTGGTGGAAACCCCATGGCTGCAG  >  NC_002947/657079‑657369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑