Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 660,592 T→C 10.2% G546G (GGT→GGC)  speA → biosynthetic arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947660,5920TC10.2% 124.1 / 5.2 49G546G (GGT→GGC) speAbiosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (19/25);  new base C (3/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

CGATGCGGCGTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTG  >  NC_002947/660444‑660739
                                                                                                                                                    |                                                                                                                                                   
cGATGCGGCGTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTggtgggt                                                                                                                                                     <  2:227306/149‑1 (MQ=255)
               tATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATc                                                                                                                                      <  7:207709/149‑1 (MQ=255)
               tATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGGGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATc                                                                                                                                      >  4:65693/1‑149 (MQ=255)
                    cAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTgg                                                                                                                                  <  8:118891/148‑1 (MQ=255)
                    cAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTgg                                                                                                                                  <  4:310417/148‑1 (MQ=255)
                        aCCTGAATTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTACTGGTGGGTGCCTAGCAGGAAATCCTGGGTGa                                                                                                                              >  4:36105/1‑148 (MQ=255)
                         ccTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACa                                                                                                                            <  2:216882/149‑1 (MQ=255)
                          cTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACAt                                                                                                                           >  1:1703/1‑149 (MQ=255)
                               ttGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGcac                                                                                                                       >  5:200647/1‑148 (MQ=255)
                                  cGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAAc                                                                                                                    <  6:200647/148‑1 (MQ=255)
                                    aTTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGATTTCCTggtag                                                                                                                               <  1:54845/135‑4 (MQ=255)
                                    aTTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGATTTCCTggtag                                                                                                                               >  2:54845/1‑132 (MQ=255)
                                     ttCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGt                                                                                                                >  2:206364/1‑149 (MQ=255)
                                        cGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCg                                                                                                             <  3:65693/149‑1 (MQ=255)
                                              cAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaa                                                                                                                                         >  6:237305/1‑115 (MQ=255)
                                              cAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaa                                                                                                                                         <  5:237305/115‑1 (MQ=255)
                                               aaGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaac                                                                                                                                        <  3:218905/115‑2 (MQ=255)
                                               aaGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaac                                                                                                                                        >  4:218905/1‑114 (MQ=255)
                                                 gATCAAGCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACAcc                                                                                                     >  2:133351/1‑148 (MQ=255)
                                                      aCCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCg                                                                                               <  2:1703/149‑1 (MQ=255)
                                                       ccAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCgg                                                                                              <  8:116437/149‑1 (MQ=255)
                                                        cAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGt                                                                                             <  5:220625/149‑1 (MQ=255)
                                                          gTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGtt                                                                                                               >  7:229112/1‑129 (MQ=255)
                                                          gTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGtt                                                                                                               <  8:229112/129‑1 (MQ=255)
                                                          gTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTACCTGGTGGGTGCCTACCAGGAAATCCAGGGTGACATGCACAACCTGTACGGTGACACCGATTCGGtc                                                                                            >  1:203555/1‑147 (MQ=255)
                                                             cGTTGACGAGCAGTGCATCGAAACCAGCATGCCGGTGCATGCGTTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGTCATGCACAACCTGTTCGGTGACACCGATTCTGTGAAc                                                                                         <  3:36105/148‑1 (MQ=255)
                                                                tGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTg                                                                                            >  2:295480/1‑142 (MQ=255)
                                                                tGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTg                                                                                            <  1:295480/142‑1 (MQ=255)
                                                                                         aTGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTa                                                            >  6:102012/1‑149 (MQ=255)
                                                                                             cGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCAc                                                        >  3:298096/1‑149 (MQ=255)
                                                                                                          gAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGaa                                            >  4:294178/1‑148 (MQ=255)
                                                                                                          gAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGaa                                            <  3:294178/148‑1 (MQ=255)
                                                                                                             ggaggGTGAGGCTTACCTGCTGGGCGTGTTCCTGGGGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAAccc                                        <  8:307731/149‑1 (MQ=255)
                                                                                                               aggGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCAc                                      <  6:206849/149‑1 (MQ=255)
                                                                                                                   tGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATc                                               <  4:96702/136‑1 (MQ=255)
                                                                                                                   tGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATc                                               >  3:96702/1‑136 (MQ=255)
                                                                                                                       gATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCAt                              <  1:206364/149‑1 (MQ=255)
                                                                                                                              tgctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGaa                          >  3:9590/1‑146 (MQ=255)
                                                                                                                              tgctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGaa                          <  4:9590/146‑1 (MQ=255)
                                                                                                                               gctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCg                                                    <  6:121432/119‑1 (MQ=255)
                                                                                                                                   caggatttCCTGGTAGGCACCCACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACAtgct                  >  1:128663/7‑149 (MQ=255)
                                                                                                                                     cgggtTCCTGGTGGGTGCCTTCCAGGAAATCCTGGGGGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCGACGACACCATCGAAGACTtgctgc                <  2:203555/146‑1 (MQ=255)
                                                                                                                                     cGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGACACCCACGACACCATCGAAGACAtgctgc                >  8:164649/1‑149 (MQ=255)
                                                                                                                                     cGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAAccc                                        <  7:163101/125‑1 (MQ=255)
                                                                                                                                     cGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAAccc                                        >  8:163101/1‑125 (MQ=255)
                                                                                                                                     cGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACAtgctgc                <  4:109137/149‑1 (MQ=255)
                                                                                                                                         ttCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGa                                    >  8:40834/1‑125 (MQ=255)
                                                                                                                                         ttCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGa                                    <  7:40834/125‑1 (MQ=255)
                                                                                                                                           ccTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTAc           <  1:133351/148‑1 (MQ=255)
                                                                                                                                            cTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGt         >  6:140909/1‑149 (MQ=255)
                                                                                                                                               gtgggtgCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCa      >  4:110753/1‑149 (MQ=255)
                                                                                                                                                   gtgCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTg  <  3:110753/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGATGCGGCGTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTG  >  NC_002947/660444‑660739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑