| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 666,886 | C→A | 10.5% | P1251H (CCC→CAC) | yfhM → | lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 666,886 | 0 | C | A | 10.5% | 105.3 / 4.6 | 38 | P1251H (CCC→CAC) | yfhM | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (17/17); new base A (2/2); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 | |||||||||||
CGAGCACTTGCTGGGGATGCAGCGCTACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGTGCTGTCGCGCA > NC_002947/666752‑667026 | cGAGCACTTGCTGGGGATGCAGCGCTACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTg > 2:267096/1‑148 (MQ=255) gAGCACTTGCTGGGGATGCAGCGCTACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTga < 7:61231/148‑1 (MQ=255) tGCAGCGCTACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAgcgc < 2:334028/149‑1 (MQ=255) cTACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCtgctgc < 6:351215/149‑1 (MQ=255) tACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCg > 3:119791/1‑149 (MQ=255) cAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGtt < 4:166823/149‑1 (MQ=255) aCGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTAc > 7:54694/1‑149 (MQ=255) ggCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCt > 5:12715/1‑149 (MQ=255) ggCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCt < 3:138585/149‑1 (MQ=255) aGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTg > 6:145210/1‑147 (MQ=255) cTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAg > 7:5339/1‑148 (MQ=255) ccTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATATGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGc > 3:92184/1‑148 (MQ=255) tgGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAAcc < 6:12715/148‑1 (MQ=255) ttCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAgc > 8:764/1‑113 (MQ=255) ttCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAgc < 7:764/113‑1 (MQ=255) ggAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGCACGCAACCTGATCGAAGTCGACTa > 8:154693/1‑147 (MQ=255) ggAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGACAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACa < 1:267096/149‑1 (MQ=255) tGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGAc < 8:54694/149‑1 (MQ=255) aCCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACca < 8:5339/149‑1 (MQ=255) gCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGtt < 6:223628/94‑1 (MQ=255) gCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGtt > 5:223628/1‑94 (MQ=255) ccTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACGAGCGTGCCCGCCGAGGCACTgaagaa > 1:70862/1‑71 (MQ=255) ccTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTgaagaa < 2:70862/70‑1 (MQ=255) cTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACAcgc > 4:83738/1‑149 (MQ=255) tgactgacTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCttt > 3:89876/1‑149 (MQ=255) ctgactTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCg < 4:92184/149‑1 (MQ=255) ctgactTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCg < 1:234713/149‑1 (MQ=255) ctTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGcc > 2:280864/1‑127 (MQ=255) ctTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGcc < 1:280864/127‑1 (MQ=255) gCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGaa > 2:568/1‑93 (MQ=255) gCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGaa < 1:568/93‑1 (MQ=255) aCCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGt < 4:89876/148‑1 (MQ=255) ctcg‑gcggGCACGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGa > 2:249245/3‑101 (MQ=255) ctcg‑gcggGCACGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGa < 1:249245/99‑1 (MQ=255) ctcg‑gcggGCACGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGTGc < 4:81403/140‑1 (MQ=255) ctcg‑gcggGCACGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGTGc > 3:81403/3‑142 (MQ=255) aCG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGTGCTGTcgcg > 8:57936/1‑148 (MQ=255) aCG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGTGC‑GTcgcttc > 7:299755/1‑146 (MQ=255) | CGAGCACTTGCTGGGGATGCAGCGCTACAACGGCAGCTTTGGCCTGTGGAGTTCGGACAGCGAGGAAGAGTACTGGCTGACCGCCTATGTGACTGACTTCCTGCTGCGTGCCCGTGACCAAGGGTACG‑GCGTGCCCGCCGAGGCACTGAAGAAGGCCAGTGAGCGCCTGCTGCGTTACCTGCAGGAACGCAACCTGATCGAAGTCGACTACAGCGAGAATGCCGACCACACGCGCTTTGCGGTGCAGGCCTACGCGGCGCTGGTGCTGTCGCGCA > NC_002947/666752‑667026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |