Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	675,591	A→T	8.9%	A36A (GCT→GCA)	PP_0577 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	675,591	0	A	T	8.9%	221.0 / 10.2	79	A36A (GCT→GCA)	PP_0577	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base T (4/3);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

GCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCCATT  >  NC_002947/675444‑675739                                                                                                                                                    |                                                                                                                                                     gCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGTCGCAGGTTAAGCACTGGCGTCGAAGGTGGTGGCCGACTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccag                                                                                                                                                     >  5:337165/1‑149 (MQ=255) gCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccag                                                                                                                                                     >  6:235190/1‑149 (MQ=255) gCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcca                                                                                                                                                      <  3:121910/148‑1 (MQ=255)  ccGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                                    >  2:262744/1‑149 (MQ=255)     ttGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                                 <  5:181167/149‑1 (MQ=255)          ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                                    >  1:245766/1‑141 (MQ=255)          ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                                    <  2:245766/141‑1 (MQ=255)            agctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCTGGCTGCCAGGGCagca                                                                                                                                          <  3:313821/149‑4 (MQ=255)            agctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATg                                                                                                                                          <  4:168685/149‑1 (MQ=255)                     tGCGTTTGTGCGCATTATGACGTGCGGGCGCAGGGTATGGAGTGGCGTAGAAGGTGGTGGCCGGCTGGGGGGGGGGCGGTGGTTACGGTCGGGTGCTGGCCAGATACGTGTTGCTGCCCTGGCAGCCAGCGAGGGCATTGGGCAGCAgg                                                                                                                                 <  6:18445/149‑1 (MQ=255)                       cGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAgg                                                                                                                                 <  4:289628/147‑1 (MQ=255)                                 cATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                                      >  2:253725/1‑148 (MQ=255)                                   tAATCACGCGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCa                                                                                                                             <  3:31471/139‑1 (MQ=255)                                   tAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCa                                                                                                                             >  4:31471/1‑139 (MQ=255)                                   tAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                                      >  7:100845/1‑146 (MQ=255)                                       cACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACgg                                                                                                               >  4:326178/1‑149 (MQ=255)                                          gAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCTGGCTGCCAGGGCa                                                                                                                                             <  4:185770/116‑1 (MQ=255)                                          gAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCTGGCTGCCAGGGCa                                                                                                                                             >  3:185770/1‑116 (MQ=255)                                          gAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTAc                                                                                                            >  8:129911/1‑149 (MQ=255)                                            gCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACgg                                                                                                          >  3:55628/1‑149 (MQ=255)                                                          aGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTccc                                                                                            <  7:129911/149‑1 (MQ=255)                                                          aGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTcc                                                                                             <  4:55628/148‑1 (MQ=255)                                                          aGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGACAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTcc                                                                                             <  5:105890/148‑1 (MQ=255)                                                            cACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTccc                                                                                            <  4:331229/147‑1 (MQ=255)                                                            cACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTccc                                                                                            >  3:331229/1‑147 (MQ=255)                                                                 gCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgc                                                                                       <  1:346261/147‑1 (MQ=255)                                                                   gTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgcggcg                                                                                   >  1:241925/1‑149 (MQ=255)                                                                    tCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgcggcg                                                                                   >  4:171176/1‑148 (MQ=255)                                                                           tggtggCCGCCTGGGCGGGGGCCGGTGGTATCGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCg                                                                           >  1:29578/1‑149 (MQ=255)                                                                           tggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGTCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCg                                                                           <  1:253725/149‑1 (MQ=255)                                                                            ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgg                                                                          >  4:198918/1‑149 (MQ=255)                                                                             gtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCTGCGGATTAGCggg                                                                         <  6:337165/149‑1 (MQ=255)                                                                             gtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgg                                                                          <  3:198918/148‑1 (MQ=255)                                                                             gtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgg                                                                          <  8:100845/148‑1 (MQ=255)                                                                              tggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgggg                                                                        <  8:29984/149‑1 (MQ=255)                                                                                gccgccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                                      <  5:200849/149‑1 (MQ=255)                                                                                ctcgccTGGGCGGGGGCCGGGGGTAACGGGCGGCGGCGGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACCGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                                      <  5:310696/147‑1 (MQ=255)                                                                                  cgccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATCAGCGGGggcgg                                                                    >  1:339838/1‑149 (MQ=255)                                                                                  cgccTGGGCGGGGGCCGGTGGTAACGGGCGGCCGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggcgg                                                                    >  5:257578/1‑149 (MQ=255)                                                                                   gccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCCGCCCGCCAGGGCAATGGGCAGCAGGGCCAGGCACACCAGCCGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                                      >  6:310696/1‑146 (MQ=255)                                                                                       gggCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGTCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAAc                                                               >  3:65669/1‑149 (MQ=255)                                                                                       gggCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAAc                                                               >  2:37403/1‑149 (MQ=255)                                                                                          cGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCa                                                            <  3:171176/149‑1 (MQ=255)                                                                                          cGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGAAGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCa                                                            <  1:262744/149‑1 (MQ=255)                                                                                          cGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTGGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCa                                                            <  2:260114/149‑1 (MQ=255)                                                                                            ggggCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGt                                                          <  3:326178/149‑1 (MQ=255)                                                                                                 cGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAgg                                                     <  5:235190/149‑1 (MQ=255)                                                                                                   gtggtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAgg                                                     >  3:216357/1‑147 (MQ=255)                                                                                                    tggtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGGTTAGCGGGGGCGGCAAACCCAGTGCAGGAAg                                                  >  5:141028/1‑149 (MQ=255)                                                                                                      gtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGc                                                 <  2:241925/148‑1 (MQ=255)                                                                                                       tAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCgg                                               >  3:216953/1‑149 (MQ=255)                                                                                                        aaCGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGc                                              >  2:17025/1‑149 (MQ=255)                                                                                                           gggCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAg                                                           >  4:289876/1‑133 (MQ=255)                                                                                                                  tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGAACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCCGCCAAGCCCGGc                                    >  8:76946/1‑149 (MQ=255)                                                                                                                    ctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCg                                   >  2:296045/1‑148 (MQ=255)                                                                                                                     tgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCg                                   >  1:117089/1‑147 (MQ=255)                                                                                                                        ccACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggt                              >  8:40318/1‑149 (MQ=255)                                                                                                                        ccACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggt                              <  2:117089/149‑1 (MQ=255)                                                                                                                         cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGTCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAg                                <  4:134665/146‑1 (MQ=255)                                                                                                                         cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAg                                >  3:134665/1‑146 (MQ=255)                                                                                                                          acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgg                            >  4:56740/1‑149 (MQ=255)                                                                                                                          acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgg                            >  6:83178/1‑149 (MQ=255)                                                                                                                           caTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                           <  7:40318/149‑1 (MQ=255)                                                                                                                            aTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgggg                          <  4:216357/149‑1 (MQ=255)                                                                                                                            aTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgggg                          <  8:235361/149‑1 (MQ=255)                                                                                                                              aCGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggggtg                        >  5:331541/1‑149 (MQ=255)                                                                                                                               cGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATCAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggggtg                        <  2:339838/148‑1 (MQ=255)                                                                                                                                gggTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGc                      >  5:15969/1‑149 (MQ=255)                                                                                                                                gggTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGc                      <  6:257578/149‑1 (MQ=255)                                                                                                                                 ggTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCg                     >  8:36844/1‑149 (MQ=255)                                                                                                                                  cccattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGc                      >  4:7106/6‑147 (MQ=255)                                                                                                                                  cccattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGc                      <  3:7106/142‑1 (MQ=255)                                                                                                                                   ttGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGAc                   >  8:203235/1‑149 (MQ=255)                                                                                                                                    cattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACg                  >  5:177605/4‑149 (MQ=255)                                                                                                                                     gctgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACg                  <  7:36844/148‑1 (MQ=255)                                                                                                                                      ctgcCCTGGCAGCCAGTCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGt                <  4:65669/149‑1 (MQ=255)                                                                                                                                      ctgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGt                <  1:17025/149‑1 (MQ=255)                                                                                                                                       tgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTg               >  1:141001/1‑149 (MQ=255)                                                                                                                                        gcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg              >  5:68919/1‑149 (MQ=255)                                                                                                                                        gcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg              >  6:206917/1‑149 (MQ=255)                                                                                                                                         cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCGAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg             >  2:285406/1‑149 (MQ=255)                                                                                                                                         cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg             >  5:96/1‑149 (MQ=255)                                                                                                                                         cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg             >  7:26479/1‑149 (MQ=255)                                                                                                                                         cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg              <  4:216953/148‑1 (MQ=255)                                                                                                                                          ccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGc            >  5:155333/1‑149 (MQ=255)                                                                                                                                              gCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGTCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGt        <  5:300029/149‑1 (MQ=255)                                                                                                                                                agccagccAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTc      >  7:272848/1‑149 (MQ=255)                                                                                                                                                    agccagGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCCAtt  >  5:126575/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCCATT  >  NC_002947/675444‑675739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑