Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	708,755	T→C	7.8%	I386T (ATC→ACC) ‡	murJ →	lipid II flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	708,755	0	T	C	7.8%	182.2 / 4.8	64	I386T (ATC→ACC) ‡	murJ	lipid II flippase
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base C (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AATATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTC  >  NC_002947/708611‑708903                                                                                                                                                 |                                                                                                                                                     aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTGTGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCa                                                                                                                                                  <  3:157891/149‑1 (MQ=255) aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCg                                                                                                                                                    >  5:338560/1‑147 (MQ=255) aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCa                                                                                                                                                  <  3:274945/149‑1 (MQ=255) aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCa                                                                                                                                                  <  3:212769/149‑1 (MQ=255)     tGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTc                                                                                                                                               <  1:104926/148‑1 (MQ=255)         aaGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCag                                                                                                                                          >  1:123317/1‑148 (MQ=255)          aGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGACTGCGATCTTCAcc                                                                                                                                         <  6:53276/149‑1 (MQ=255)           gTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGACTGCGATCTTCAccg                                                                                                                                        <  1:346142/149‑2 (MQ=255)               aGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGt                                                                                                                                    <  2:123317/149‑1 (MQ=255)                gcgcGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTc                                                                                                                                   <  6:259891/149‑1 (MQ=255)                gcgcGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTc                                                                                                                                   >  1:180012/1‑149 (MQ=255)                 cgcgTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCt                                                                                                                                  >  2:172160/1‑149 (MQ=255)                    gTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCa                                                                                                                               <  8:250997/149‑1 (MQ=255)                                     cccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGACTGCGATCTTCAccg                                                                                                                                        <  3:221639/123‑2 (MQ=255)                                     cccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGACTGCGATCTTCAccg                                                                                                                                        >  4:221639/1‑122 (MQ=255)                                      ccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTg                                                                                                             >  5:248032/1‑149 (MQ=255)                                      ccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTg                                                                                                             >  3:46912/1‑149 (MQ=255)                                      ccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTg                                                                                                             >  7:262413/1‑149 (MQ=255)                                         gCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGccc                                                                                                          <  6:248032/149‑1 (MQ=255)                                                  gATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGTCTTCTATGCGCAGCAGAATATTCGCACGCCCGTGAAGATCGCAGTCTACACACTGGTCTGCACCCTGCTGTTCAACATTGCCCTGGTCGGcc                                                                                                 >  8:324462/1‑149 (MQ=255)                                                   aTCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGccc                                                                                                <  6:338560/149‑1 (MQ=255)                                                    tCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGc                                                                                                                         <  4:27641/123‑1 (MQ=255)                                                    tCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGc                                                                                                                         >  3:27641/1‑123 (MQ=255)                                                       ccTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCtt                                                                                            >  4:156471/1‑149 (MQ=255)                                                       ccTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCtt                                                                                            >  1:106485/1‑149 (MQ=255)                                                           ttCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGc                                                                                          <  8:262413/147‑1 (MQ=255)                                                                    cTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGcc                                                                               >  7:105980/1‑149 (MQ=255)                                                                       gCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTgg                                                                            >  7:27745/1‑149 (MQ=255)                                                                          ggCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCAt                                                                         <  2:169134/149‑1 (MQ=255)                                                                             gATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCtt                                                                                                              <  2:194504/109‑1 (MQ=255)                                                                             gATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTgg                                                                      <  2:106485/149‑1 (MQ=255)                                                                             gATCATTCTGATCAAGGTACTGGCACCTCGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCtt                                                                                                              >  1:194504/1‑109 (MQ=255)                                                                              aTCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGc                                                                     <  8:27745/149‑1 (MQ=255)                                                                                 aTTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGAt                                                                  >  5:197059/1‑149 (MQ=255)                                                                                  ttCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCAGGCCGGCAtgt                                                                       <  3:211865/143‑3 (MQ=255)                                                                                  ttCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCAGGCCGGCAtgt                                                                       >  4:211865/1‑141 (MQ=255)                                                                                                 tGGCACCTGGCTTCTATGCGCAGCAGAATTTTTGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgcctgc                                                  >  3:153559/1‑149 (MQ=255)                                                                                                 tGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTg                                                                                                        >  8:134464/1‑95 (MQ=255)                                                                                                 tGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTg                                                                                                        <  7:134464/95‑1 (MQ=255)                                                                                                 tGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgcctgc                                                  >  8:294857/1‑149 (MQ=255)                                                                                                  ggCACCTGGCTTCTATGTGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgcctgcc                                                 >  2:81781/1‑149 (MQ=255)                                                                                                          gCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCg                                                                                  >  8:264259/1‑108 (MQ=255)                                                                                                          gCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCg                                                                                  <  7:264259/108‑1 (MQ=255)                                                                                                          gCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGcc                                         <  1:172160/149‑1 (MQ=255)                                                                                                           cTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCg                                        <  3:156471/149‑1 (MQ=255)                                                                                                             tCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGt                                      >  7:232842/1‑149 (MQ=255)                                                                                                              cTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAAt                                            >  4:46237/1‑142 (MQ=255)                                                                                                              cTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAAt                                            <  3:46237/142‑1 (MQ=255)                                                                                                              cTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGtt                                     >  7:11955/1‑149 (MQ=255)                                                                                                                    gcagcaGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGt                               >  6:84191/1‑149 (MQ=255)                                                                                                                      agcagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTc                             >  5:124070/1‑149 (MQ=255)                                                                                                                       gcagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCt                            <  2:180012/149‑1 (MQ=255)                                                                                                                       gcagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCt                            <  8:11955/149‑1 (MQ=255)                                                                                                                        cagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCCGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCTATGAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTg                           <  4:139311/149‑1 (MQ=255)                                                                                                                           aaTATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCATCCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTTCTGTTCTGGaa                        <  7:324462/149‑1 (MQ=255)                                                                                                                                tcacACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgc                   >  3:250714/4‑149 (MQ=255)                                                                                                                                tCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgc                   >  7:288628/1‑149 (MQ=255)                                                                                                                                  gCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgcgc                 <  1:81781/149‑1 (MQ=255)                                                                                                                                  gCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgcg                  >  2:270525/1‑148 (MQ=255)                                                                                                                                  acACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgcg                  <  4:250714/147‑1 (MQ=255)                                                                                                                                     cGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGt              <  4:46912/149‑1 (MQ=255)                                                                                                                                      gCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTc             <  4:153559/149‑1 (MQ=255)                                                                                                                                         ggTGAAGACTGCGATCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTcag           >  3:298927/1‑148 (MQ=255)                                                                                                                                            gAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCagcagc       >  4:38196/1‑149 (MQ=255)                                                                                                                                                 tCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGATc  >  3:236128/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                     AATATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTC  >  NC_002947/708611‑708903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑