Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 772,182 C→A 8.7% intergenic (+43/‑156) PP_0663 → / → PP_0664 AsnC family transcriptional regulator/homoserine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947772,1820CA 8.7% 186.8 / 4.6 62intergenic (+43/‑156)PP_0663/PP_0664AsnC family transcriptional regulator/homoserine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base A (4/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTGGACAGGA  >  NC_002947/772040‑772328
                                                                                                                                              |                                                                                                                                                  
cTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAg                                                                                                                                              >  1:36751/1‑149 (MQ=255)
     gCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAggcggc                                                                                                                                         >  5:150504/1‑149 (MQ=255)
      cccGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGgcggcg                                                                                                                                        <  3:49184/149‑1 (MQ=255)
       ccGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGt                                                                                                                                       >  5:78589/1‑149 (MQ=255)
          gAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTtgt                                                                                                                                    <  6:148136/149‑1 (MQ=255)
                    aCCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGcc                                                                                                                          >  1:58291/1‑149 (MQ=255)
                            tgGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGgcg                                                                                                                    >  2:77842/1‑147 (MQ=255)
                            tcgaaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTAAGTCCTGGCCGCGGAGGTCTGAACCGCCTCTTTGCATTGGCTAGCACAACGCCGCCCGGGTTCGCGCCCACGAGCCGTTGTGCGTATTGCCCTGgcgc                                                                                                                   >  4:43807/3‑148 (MQ=255)
                              gaaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAg                                                                                                                 <  6:38434/148‑1 (MQ=255)
                               aaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGt                                                                                                               >  7:251060/1‑149 (MQ=255)
                               aaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTGTTGCCCTGGCGCAGGt                                                                                                               >  8:262508/1‑149 (MQ=255)
                               aaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAggc                                                                                                                                            >  8:30436/1‑120 (MQ=39)
                               aaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAggc                                                                                                                                            <  7:30436/120‑1 (MQ=39)
                                 ggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTgc                                                                                                             >  7:272266/1‑149 (MQ=255)
                                 ggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGGTGGCTGAACCGGGTCTTTGCTTTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTgc                                                                                                             <  7:301641/149‑1 (MQ=255)
                                        gCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCttt                                                                                                      >  8:258392/1‑149 (MQ=255)
                                              aTGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTg                                                                                                <  3:92428/149‑1 (MQ=255)
                                              aTGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGAGCGCTTTGCACTg                                                                                                >  6:19580/1‑149 (MQ=255)
                                                 cccGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGcc                                                                                             <  5:19580/149‑1 (MQ=255)
                                                  ccGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGc                                                                                              >  2:229044/1‑147 (MQ=255)
                                                  ccGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCa                                                                                            >  4:67381/1‑149 (MQ=255)
                                                  ccGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGAAGGTGCGCGCTTTGCACTGGc                                                                                              >  8:164665/1‑147 (MQ=255)
                                                   cGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAg                                                                                           <  7:169291/149‑1 (MQ=255)
                                                     gggTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGAt                                                                                         >  6:284863/1‑149 (MQ=255)
                                                         ggAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGc                                                                                     <  7:314692/149‑1 (MQ=255)
                                                               cacGGTGACTTCGTTCGTCCTGGCCGCGCTGGTCTGAAACGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGAGCCCAGGCGGCGTTGTGCGTTTTGCCCTGCCGAAGGTGCGCGCTATCCACTGGACAGATTGGCCaaaa                                                                                >  7:136286/1‑146 (MQ=255)
                                                                acGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCaataaa                                                                              <  8:251060/149‑1 (MQ=255)
                                                                    tGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACa                                                                          <  2:5156/149‑1 (MQ=255)
                                                                        ttcgttcgTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCg                                                                       >  8:242178/1‑148 (MQ=255)
                                                                           gttcgtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGAtt                                                                   >  7:199576/1‑149 (MQ=255)
                                                                            ttcgtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTg                                                                  <  6:78589/149‑1 (MQ=255)
                                                                              cgtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGc                                                                 <  7:262508/148‑1 (MQ=255)
                                                                               gtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCGGCCCGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGcc                                                                <  3:43807/148‑1 (MQ=255)
                                                                                     gCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTc                                                          >  5:98854/1‑148 (MQ=255)
                                                                                        tcgttggtCTGAACCGCGTCTTTGCAGTGTCTAGAACAACTCCTCCTGGTTTCGCTCCCAGGCGGCTTTGTGCGTTTTGCCCTGGCGCAGTTTAGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAAc                                                       <  2:212958/144‑1 (MQ=255)
                                                                                        gcgGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                      <  4:178474/149‑1 (MQ=255)
                                                                                        gcgGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                      <  4:173844/149‑1 (MQ=255)
                                                                                        gcgGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                      <  2:58291/149‑1 (MQ=255)
                                                                                          ggtggtCTCAACCGCGTATTTGCATTGGATAGCACAACGCCGCCTGCGTTCGCTCCCACTCGCCGTTCTGAGATTTGCCCTCGAGCACATGCGAGCATTGCACTGGCCAGATTGGCCAATAAATACAGAGGATTGCCATATTCAAc                                                       >  1:212958/1‑146 (MQ=255)
                                                                                              gtCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGt                                                >  6:128151/1‑149 (MQ=255)
                                                                                               tCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGt                                                <  6:150504/148‑1 (MQ=255)
                                                                                                 tGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCa                                             >  8:245364/1‑149 (MQ=255)
                                                                                                  gAAGCGCGTCTTTGCATTGGCTAGCACAACGCCGGCTGGGTTCGCGCGCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCa                                             <  8:136286/148‑1 (MQ=255)
                                                                                                   aaacgagTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTAAACTGGCAGTGAACg                                           >  7:217474/7‑149 (MQ=255)
                                                                                                   aaCCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACg                                           <  7:242178/149‑1 (MQ=255)
                                                                                                      cgcgTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGct                                        >  3:147713/1‑149 (MQ=255)
                                                                                                             ttGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGc                                 >  5:20052/1‑149 (MQ=255)
                                                                                                              tGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTAc                                   <  7:245364/146‑1 (MQ=255)
                                                                                                                    ggCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTcc                           <  1:77842/148‑1 (MQ=255)
                                                                                                                       tAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCAt                       >  2:134129/1‑149 (MQ=255)
                                                                                                                       tAGCACAACGCCGCCTGGGTTCGCGCACAGGCGGCGTTGTGAGTTTTTCCCTGGCGCAGGTGCGCGCTTTGAACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTCGCAGTGCACGCGTCTTACGCTAGTACTCAt                       >  7:123149/1‑149 (MQ=255)
                                                                                                                          cacaACGCCGCGTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCa                        <  4:50112/145‑1 (MQ=255)
                                                                                                                          cacaACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCa                        >  3:50112/1‑145 (MQ=255)
                                                                                                                           acaACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGAc                   >  5:337461/1‑149 (MQ=255)
                                                                                                                                gccgccTGGGTTCGCGCCCAGGCGTCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACt              <  6:98854/149‑1 (MQ=255)
                                                                                                                                gccgccTGGGCGCGAACCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACt              >  5:128642/1‑149 (MQ=255)
                                                                                                                                 ccgccTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTg             <  8:199576/149‑1 (MQ=255)
                                                                                                                                  cgccTGGGCGCGAACCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGc            >  7:45355/1‑149 (MQ=255)
                                                                                                                                   gccTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGcc           <  1:229044/149‑1 (MQ=255)
                                                                                                                                    ccTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCt          <  7:164665/149‑1 (MQ=255)
                                                                                                                                     cTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTg         <  2:36751/149‑1 (MQ=255)
                                                                                                                                         gTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTggaca     <  1:68819/149‑1 (MQ=255)
                                                                                                                                             gcgcCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTggacagga  >  2:243760/1‑148 (MQ=255)
                                                                                                                                             gcgcCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTggacagga  >  4:78435/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTGGACAGGA  >  NC_002947/772040‑772328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑