Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 830,867 G→C 7.8% A47A (GCG→GCC)  PP_0715 → outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947830,8670GC 7.8% 187.8 / 2.3 60A47A (GCG→GCC) PP_0715outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base C (2/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCCCAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGAAGCCCGTGCCCAGTTGGCCATCG  >  NC_002947/830726‑831009
                                                                                                                                             |                                                                                                                                              
atctcccAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGgcgc                                                                                                                                         <  7:279202/145‑1 (MQ=255)
    cccAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCTGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCCGCCGCGCTGCGccct                                                                                                                                      <  5:289776/148‑2 (MQ=255)
     ccAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCACGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGgcgc                                                                                                                                         >  8:279202/1‑144 (MQ=255)
     ccAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGAc                                                                                                                                    >  4:41398/1‑149 (MQ=255)
      cAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGAcc                                                                                                                                   >  4:124095/1‑149 (MQ=255)
      cAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGAcc                                                                                                                                   >  8:41263/1‑149 (MQ=255)
       aaCCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCATGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCt                                                                                                                                  >  2:32594/1‑149 (MQ=255)
         ccGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGc                                                                                                                                >  3:223882/1‑149 (MQ=255)
               ctATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGTCGGGCGCCGCCGCGCTGCGCCctgcctgctcgat                                                                                                                           <  5:283348/148‑6 (MQ=37)
               ctATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCa                                                                                                                            <  3:124095/147‑1 (MQ=255)
               ctATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAgt                                                                                                                          >  6:126597/1‑149 (MQ=255)
                  tGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAgtgg                                                                                                                        <  5:194417/148‑1 (MQ=255)
                     ccTGGGGCTGCTGATGGTGCTGGCCGGTTGCACACAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTggg                                                                                                                    >  1:221549/1‑149 (MQ=255)
                       tGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCg                                                                                                                  >  6:223946/1‑149 (MQ=255)
                         gggCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCg                                                                                                                  <  7:313178/147‑1 (MQ=255)
                               ctgATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGGGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCgcggcg                                                                                                                                             >  3:72099/1‑114 (MQ=255)
                               ctgATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCgcggcg                                                                                                                                             <  4:72099/114‑1 (MQ=255)
                               ctgATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGttt                                                                                                            <  1:197303/147‑1 (MQ=255)
                               ctgATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGttt                                                                                                            <  7:41263/147‑1 (MQ=255)
                               ctgATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGttt                                                                                                            <  2:206953/147‑1 (MQ=255)
                               ctgATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCAGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGc                                                                                                          >  1:227006/1‑149 (MQ=255)
                                  aTGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGcc                                                                                                         <  5:296562/147‑1 (MQ=255)
                                   tGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACTGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGAACTGCGTCAGTGGTGGGCGGTGTTTGCCGAc                                                                                                      >  6:343840/1‑149 (MQ=255)
                                   tGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGAc                                                                                                      <  4:284641/149‑1 (MQ=255)
                                          ctctggTTGCACTCTGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTTGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTTGTGGGCGGTGTTTGCCGACTCGAcc                                                                                                <  6:294885/144‑1 (MQ=255)
                                             cGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCACCAAGCGCCGTGGATGGACGGTTGGAGAACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGTCCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGAcc                                                                                                >  5:294885/1‑145 (MQ=255)
                                                ttGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGAc                                                                                                 <  7:69164/141‑1 (MQ=255)
                                                ttGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGAc                                                                                                 >  8:69164/1‑141 (MQ=255)
                                                ttGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGAcg                                                                                         <  4:223882/149‑1 (MQ=255)
                                                   ctctctGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACgcgc                                                                                      <  2:227006/147‑1 (MQ=255)
                                                    aCTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCt                                                                                     >  8:138967/1‑149 (MQ=255)
                                                     cTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCt                                                                                     >  4:154243/1‑148 (MQ=255)
                                                        aGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGAt                                                                                  <  4:196459/148‑1 (MQ=255)
                                                              gTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGc                                                                               >  3:169121/1‑145 (MQ=255)
                                                              gTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGc                                                                               <  4:169121/145‑1 (MQ=255)
                                                               tCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACgcgc                                                                                      >  4:63390/1‑137 (MQ=255)
                                                               tCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACgcgc                                                                                      <  3:63390/137‑1 (MQ=255)
                                                                 cGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGcc                                                                        <  5:126597/149‑1 (MQ=255)
                                                                 cGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCAGGATCGAGCAGGCAGGGCGCAGCGCGGAGGCGCCCGACATGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGcc                                                                        >  2:302081/1‑149 (MQ=255)
                                                                  ggATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGcc                                                                        >  3:42210/1‑148 (MQ=255)
                                                                          agaagaCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCCGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCcaa                                                                >  7:308842/1‑148 (MQ=255)
                                                                          agaAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCcaac                                                               <  3:41398/149‑1 (MQ=255)
                                                                               ccccAAGCGCCGTGGCTGGACGGTTGGAGGACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCcaac                                                               <  6:76641/144‑1 (MQ=255)
                                                                               ccccAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCcaac                                                               >  5:76641/1‑144 (MQ=255)
                                                                                cccAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACAcc                                                         <  7:138967/149‑1 (MQ=255)
                                                                                  cAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCaa                                                       >  1:34452/1‑149 (MQ=255)
                                                                                   aaGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAAc                                                      <  4:42210/149‑1 (MQ=255)
                                                                                           tGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGt                                              <  2:221549/149‑1 (MQ=255)
                                                                                             gCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGttt                                                                                                            <  4:244786/85‑1 (MQ=255)
                                                                                             gCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGttt                                                                                                            >  3:244786/1‑85 (MQ=255)
                                                                                               tGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGtggct                                          >  5:98176/1‑149 (MQ=255)
                                                                                                            cACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCg                             >  3:235377/1‑149 (MQ=255)
                                                                                                                 ccATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGaa                        <  2:34452/149‑1 (MQ=255)
                                                                                                                      aTCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGc                                   >  3:307/1‑133 (MQ=255)
                                                                                                                      aTCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGc                                   <  4:307/133‑1 (MQ=255)
                                                                                                                         gAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGAAGCCCGTg                 <  1:32594/148‑1 (MQ=255)
                                                                                                                          aGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGCCCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACCCCAACCTGCGCGTGGCTGGCCTACGTATCGCTGAAGCCCGTGcc               >  2:262711/1‑149 (MQ=255)
                                                                                                                           gcaggtcgGGCGCCGCCGCGCTGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGc                                                                                                          <  6:322895/57‑1 (MQ=9)
                                                                                                                           gcaggtcgGGCGCCGCCGCGCTGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGc                                                                                                          >  5:322895/1‑57 (MQ=9)
                                                                                                                              ggcaggGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGAAGCCCGTGCCCAGt           >  4:337836/1‑149 (MQ=255)
                                                                                                                               gcaggGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGAAGCCCGTGCCCAGtt          <  5:343840/149‑1 (MQ=255)
                                                                                                                                       cAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGAAGCCCGTGCCCAGTTGGCCATCg  >  1:176159/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CATGCCCAACCGCTGCTATGGCCTGGGGCTGCTGATGGTGCTGGCCGGTTGCACTCAGGTTGGTCCGGATTTCGAGAAGCCCCAAGCGCCGTGGCTGGACGGTTGGAGCACGCCCATGATCGAGCAGGCAGGGCGCAGCGCGGCGGCGCCCGACCTGCGTCAGTGGTGGGCGGTGTTTGCCGACTCGACCCTGGACGCGCTGATAGCCGAGGCCGATGCCAACAACACCAACCTGCGCGTGGCTGGCCTGCGTATCGCTGAAGCCCGTGCCCAGTTGGCCATCG  >  NC_002947/830726‑831009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑