Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	104,876	G→C	7.6%	P345P (CCG→CCC)	PP_0099 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	104,876	0	G	C	7.6%	278.7 / 9.5	91	P345P (CCG→CCC)	PP_0099	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/44);  new base C (4/3);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCC  >  NC_002947/104733‑105019                                                                                                                                                |                                                                                                                                                tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGt                                                                                                                                              <  4:196580/147‑1 (MQ=255) tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTgg                                                                                                                                            >  4:261488/1‑149 (MQ=255) tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTg                                                                                                                                             <  3:327272/148‑1 (MQ=255) tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTCCCGAGTTGCTGGCGCAGCACTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTgg                                                                                                                                            >  1:244466/1‑149 (MQ=255)     aCAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                           >  6:252462/1‑146 (MQ=255)      cAGCCGGTTCGGCGTGCGCATGCATGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGa                                                                                                                                       <  4:222515/149‑1 (MQ=255)            gTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                           <  8:182775/139‑1 (MQ=255)            gTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                           >  7:182775/1‑139 (MQ=255)                ggCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGtt                                                                                                                             <  4:55173/149‑1 (MQ=255)                   gTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGc                                                                                                                                          >  8:177769/1‑133 (MQ=255)                   gTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGc                                                                                                                                          <  7:177769/133‑1 (MQ=255)                   gTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTg                                                                                                                          >  4:257910/1‑149 (MQ=255)                     gcgcATGCGTGGTGAAGGAGTATTTGCCGAGTTTCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                        <  4:286359/149‑1 (MQ=255)                      cgcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                        <  5:8874/148‑1 (MQ=255)                      cgcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                        <  5:206612/148‑1 (MQ=255)                       gcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGgcg                                                                                                                                  <  2:263639/137‑1 (MQ=255)                       gcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGgcg                                                                                                                                  >  1:263639/1‑137 (MQ=255)                       gcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACAt                                                                                                                      >  1:293473/1‑149 (MQ=255)                            cGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAacc                                                                                                                 >  7:243158/1‑149 (MQ=255)                                     ggAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAccccc                                                                                                         >  4:7050/1‑148 (MQ=255)                                     ggAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAccccc                                                                                                         >  3:69116/1‑148 (MQ=255)                                        gTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTAGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCca                                                                                                       >  3:235250/1‑147 (MQ=255)                                           tttGCCGAGTTGCTGGCGCAGCACTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                  <  2:244466/149‑1 (MQ=255)                                             tGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgggg                                                                                                 <  7:96054/148‑1 (MQ=255)                                              gCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGc                                                                                               >  8:241474/1‑149 (MQ=255)                                              gCCGAGTTGCGGGCGCAGCGCTTTGCCAAGGCAATGAAACGTCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTGGGCGTTTTGCCCACCGGGTGTGCAGATGGCGCTGTTCTGACATGAAGCCCACCCCCCACAggggg                                                                                                <  6:92524/148‑1 (MQ=255)                                               ccGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCt                                                                                              <  2:293473/149‑1 (MQ=255)                                               ccGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCt                                                                                              >  8:151069/1‑149 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCTAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                         <  4:98830/114‑1 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCTAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                         >  3:98830/1‑114 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                      <  3:261488/149‑1 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                      <  4:69116/149‑1 (MQ=255)                                                        tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCt                                                                                                                                >  6:188860/1‑106 (MQ=255)                                                          gcgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCt                                                                                    <  3:257910/148‑1 (MQ=255)                                                          gcgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCa                                                                                                                                         <  7:261348/95‑1 (MQ=255)                                                          gcgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCa                                                                                                                                         >  8:261348/1‑95 (MQ=255)                                                           cgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTca                                                                                  <  3:7050/149‑1 (MQ=255)                                                            gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGGGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTca                                                                                  >  1:242621/1‑148 (MQ=255)                                                            gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                  <  3:4499/132‑1 (MQ=255)                                                            gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                  >  4:4499/1‑132 (MQ=255)                                                             cAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATTGCGCTGTTCTGACATGAACCCCACCCCCCACAGTGGGCTGCGCATTGCTcaca                                                                                <  4:235250/149‑1 (MQ=255)                                                             cAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCAGCGCATTg                                                                                      <  7:251943/143‑1 (MQ=255)                                                             cAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCAGCGCATTg                                                                                      >  8:251943/1‑143 (MQ=255)                                                                       ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTg                                                                                             >  4:260713/1‑126 (MQ=255)                                                                       ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTg                                                                                             <  3:260713/126‑1 (MQ=255)                                                                       ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                      >  1:72963/1‑133 (MQ=255)                                                                       ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                      <  2:72963/133‑1 (MQ=255)                                                                        cAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                      >  8:238528/1‑148 (MQ=255)                                                                         aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                      >  5:21688/1‑147 (MQ=255)                                                                         aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                      <  6:21688/147‑1 (MQ=255)                                                                          aGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTc                                                                                   <  3:14552/133‑1 (MQ=255)                                                                          aGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTc                                                                                   >  4:14552/1‑133 (MQ=255)                                                                          aGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaataa                                                                   >  5:280993/1‑149 (MQ=255)                                                                             cAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacaa                                                                <  2:67881/149‑1 (MQ=255)                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacaac                                                               <  7:151069/149‑1 (MQ=255)                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacaac                                                               >  2:39195/1‑149 (MQ=255)                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAAATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacaa                                                                >  5:267234/1‑148 (MQ=255)                                                                                 gAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa                                                             >  7:7675/1‑148 (MQ=255)                                                                                  aaaCGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaa                                                            >  6:154690/1‑148 (MQ=255)                                                                                         tGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgcg                                                    >  3:95514/1‑149 (MQ=255)                                                                                              ttCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgcgc                                                   <  2:126724/145‑1 (MQ=255)                                                                                              ttCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgcgc                                                   >  1:126724/1‑145 (MQ=255)                                                                                               tCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATa                                              >  7:322008/1‑149 (MQ=255)                                                                                                cGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATa                                              >  5:273936/1‑148 (MQ=255)                                                                                                  aaGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTCTTCTGACATGAACCCCACCCCCCACAGGGGGCTCCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACa                                            >  5:13478/1‑148 (MQ=255)                                                                                                    gggCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACatat                                         <  1:39195/149‑1 (MQ=255)                                                                                                    gggCGCGAGGCGCAGGAGCTGGATCGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACatat                                         >  4:273682/1‑149 (MQ=255)                                                                                                     ggCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATAtt                                        <  7:16229/149‑1 (MQ=255)                                                                                                      gcgcGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTTGGCAGATGGAGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTg                                       <  5:252462/149‑1 (MQ=255)                                                                                                      gcgcGAGGCGCAGGAGCTGGATCGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTg                                       <  3:273682/149‑1 (MQ=255)                                                                                                        gcgAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCCCCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTgg                                      >  3:97744/1‑148 (MQ=255)                                                                                                          gAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCGCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCaa                                   <  6:267234/149‑1 (MQ=255)                                                                                                          gAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCaa                                   <  7:241474/149‑1 (MQ=255)                                                                                                           aGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAAc                                  <  4:95514/149‑1 (MQ=255)                                                                                                                         gATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGttttttt                      <  6:280993/147‑1 (MQ=255)                                                                                                                         gATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGttttttt                      <  7:8356/147‑1 (MQ=255)                                                                                                                         gATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTg                     <  8:243158/148‑1 (MQ=255)                                                                                                                           ttGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCta                  <  2:242621/149‑1 (MQ=255)                                                                                                                                 ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATTATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc             >  8:208096/1‑148 (MQ=255)                                                                                                                                 ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATcc            <  8:322008/149‑1 (MQ=255)                                                                                                                                 ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc             >  8:45599/1‑148 (MQ=255)                                                                                                                                 ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc             <  5:154690/148‑1 (MQ=255)                                                                                                                                  gCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCa           <  7:238528/149‑1 (MQ=255)                                                                                                                                     atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGa                               >  1:162870/4‑126 (MQ=255)                                                                                                                                     atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGa                               <  2:162870/123‑1 (MQ=255)                                                                                                                                     atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc             >  2:23727/4‑144 (MQ=255)                                                                                                                                     atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc             <  1:23727/141‑1 (MQ=255)                                                                                                                                     atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGAAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGtt        >  2:34959/4‑149 (MQ=255)                                                                                                                                         gCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCg    >  1:229745/1‑149 (MQ=255)                                                                                                                                           ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGcc  <  8:7675/149‑1 (MQ=255)                                                                                                                                           ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCATCGCATTTCTAAAAAAGCAACAAATAAAAAACAATAATTTCGCTAATAAATATTTGCAAAGTATGGTTTTTTGGTTTTTTACAGTTACCggc  >  8:143468/1‑147 (MQ=255)                                                                                                                                                |                                                                                                                                                TATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCC  >  NC_002947/104733‑105019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑