Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 856,078 G→A 8.0% A9A (GCG→GCA)  pagL‑I → lipid A 3‑O‑deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947856,0780GA 8.0% 141.9 / 2.2 50A9A (GCG→GCA) pagL‑Ilipid A 3‑O‑deacylase
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base A (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

AAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGT  >  NC_002947/855947‑856221
                                                                                                                                   |                                                                                                                                               
aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaagccga                                                                                                                                 <  6:288870/148‑8 (MQ=255)
 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                               <  4:25466/149‑1 (MQ=255)
 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                               <  4:150037/149‑1 (MQ=255)
 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                               <  6:186739/149‑1 (MQ=255)
  aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                              <  1:168862/149‑1 (MQ=255)
  aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                              >  3:16813/1‑149 (MQ=255)
      aCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTggggt                                                                                                                           >  8:264883/1‑147 (MQ=255)
                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                         >  7:217541/1‑120 (MQ=255)
                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                         <  8:217541/120‑1 (MQ=255)
                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGca                                                                                                             >  4:305376/1‑148 (MQ=255)
                           tCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGc                                                                                                     <  7:257820/149‑1 (MQ=255)
                             gTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGcc                                                                                                    >  4:55311/1‑148 (MQ=255)
                              ttCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGa                                                                                                  <  4:16813/149‑1 (MQ=255)
                                    ttCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                            >  6:267651/1‑149 (MQ=255)
                                      cTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGt                                                                                          >  2:87989/1‑149 (MQ=255)
                                         cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGcc                                                                                                                                        <  6:114127/100‑1 (MQ=255)
                                         cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGcc                                                                                                                                        >  5:114127/1‑100 (MQ=255)
                                         cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGCAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGtt                                                                                         <  1:109244/147‑1 (MQ=255)
                                                ccGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                 <  5:267651/148‑1 (MQ=255)
                                                 cGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                     >  6:303546/1‑143 (MQ=255)
                                                 cGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                     <  5:303546/143‑1 (MQ=255)
                                                      tGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGa                                                                           <  4:75463/148‑1 (MQ=255)
                                                      tGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGa                                                                           <  3:305376/148‑1 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                               >  8:240316/1‑109 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                               <  7:240316/109‑1 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTCGGGCAGACTgg                                                                       >  6:164179/1‑149 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTAGGTGGGGCAGACTgg                                                                       >  3:298612/1‑149 (MQ=255)
                                                          tGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGc                                                                      >  2:48952/1‑149 (MQ=255)
                                                          tGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGc                                                                      >  5:318839/1‑149 (MQ=255)
                                                               tGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                 <  7:264883/149‑1 (MQ=255)
                                                                 aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGc                                                                              <  7:278860/134‑1 (MQ=255)
                                                                 aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGc                                                                              >  8:278860/1‑134 (MQ=255)
                                                                  ggCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGa                                                               >  1:342672/1‑148 (MQ=255)
                                                                     ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                           >  7:5171/1‑149 (MQ=255)
                                                                     ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                           <  3:55311/149‑1 (MQ=255)
                                                                                   aTCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAg                                             >  4:231137/1‑149 (MQ=255)
                                                                                    tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAgg                                            >  8:159754/1‑149 (MQ=255)
                                                                                          agaagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGc                                       >  5:3763/1‑148 (MQ=255)
                                                                                          agaagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCa                                      <  3:231137/149‑1 (MQ=255)
                                                                                                  gTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                 <  3:244524/114‑1 (MQ=255)
                                                                                                  gTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                 >  4:244524/1‑114 (MQ=255)
                                                                                                                  cTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGGTGCGGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtg              <  1:308993/149‑1 (MQ=255)
                                                                                                                   tGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg             >  5:50679/1‑149 (MQ=255)
                                                                                                                     cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg             <  6:318839/147‑1 (MQ=255)
                                                                                                                     cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg             <  1:48952/147‑1 (MQ=255)
                                                                                                                      tCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg             >  1:75609/1‑146 (MQ=255)
                                                                                                                        ggCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc        >  4:198916/1‑149 (MQ=255)
                                                                                                                         caaaggCAGCAGCCGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc        >  5:281749/5‑148 (MQ=255)
                                                                                                                          cTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc        >  3:183552/1‑147 (MQ=255)
                                                                                                                              gCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGt  <  6:50679/149‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                               
AAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGT  >  NC_002947/855947‑856221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑