Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 856,081 T→C 8.3% A10A (GCT→GCC)  pagL‑I → lipid A 3‑O‑deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947856,0810TC 8.3% 130.6 / 2.9 48A10A (GCT→GCC) pagL‑Ilipid A 3‑O‑deacylase
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base C (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

AAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGG  >  NC_002947/855947‑856228
                                                                                                                                      |                                                                                                                                                   
aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaagccga                                                                                                                                        <  6:288870/148‑8 (MQ=255)
 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                      <  6:186739/149‑1 (MQ=255)
 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                      <  4:25466/149‑1 (MQ=255)
 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                      <  4:150037/149‑1 (MQ=255)
  aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                     <  1:168862/149‑1 (MQ=255)
  aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                     >  3:16813/1‑149 (MQ=255)
      aCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTggggt                                                                                                                                  >  8:264883/1‑147 (MQ=255)
                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                                <  8:217541/120‑1 (MQ=255)
                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                                >  7:217541/1‑120 (MQ=255)
                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGca                                                                                                                    >  4:305376/1‑148 (MQ=255)
                           tCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGc                                                                                                            <  7:257820/149‑1 (MQ=255)
                             gTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGcc                                                                                                           >  4:55311/1‑148 (MQ=255)
                              ttCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGa                                                                                                         <  4:16813/149‑1 (MQ=255)
                                    ttCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                                   >  6:267651/1‑149 (MQ=255)
                                      cTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGt                                                                                                 >  2:87989/1‑149 (MQ=255)
                                         cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGcc                                                                                                                                               <  6:114127/100‑1 (MQ=255)
                                         cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGcc                                                                                                                                               >  5:114127/1‑100 (MQ=255)
                                         cTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGCAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGtt                                                                                                <  1:109244/147‑1 (MQ=255)
                                                ccGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                        <  5:267651/148‑1 (MQ=255)
                                                 cGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                            <  5:303546/143‑1 (MQ=255)
                                                 cGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCg                                                                                            >  6:303546/1‑143 (MQ=255)
                                                      tGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGa                                                                                  <  3:305376/148‑1 (MQ=255)
                                                      tGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGa                                                                                  <  4:75463/148‑1 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                      >  8:240316/1‑109 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                      <  7:240316/109‑1 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTCGGGCAGACTgg                                                                              >  6:164179/1‑149 (MQ=255)
                                                         cTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTAGGTGGGGCAGACTgg                                                                              >  3:298612/1‑149 (MQ=255)
                                                          tGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGc                                                                             >  2:48952/1‑149 (MQ=255)
                                                          tGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGc                                                                             >  5:318839/1‑149 (MQ=255)
                                                               tGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                        <  7:264883/149‑1 (MQ=255)
                                                                 aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGc                                                                                     <  7:278860/134‑1 (MQ=255)
                                                                 aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGc                                                                                     >  8:278860/1‑134 (MQ=255)
                                                                  ggCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGa                                                                      >  1:342672/1‑148 (MQ=255)
                                                                     ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                                  >  7:5171/1‑149 (MQ=255)
                                                                     ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                                  <  3:55311/149‑1 (MQ=255)
                                                                                   aTCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAg                                                    >  4:231137/1‑149 (MQ=255)
                                                                                    tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAgg                                                   >  8:159754/1‑149 (MQ=255)
                                                                                          agaagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGc                                              >  5:3763/1‑148 (MQ=255)
                                                                                          agaagGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCa                                             <  3:231137/149‑1 (MQ=255)
                                                                                                  gTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                        <  3:244524/114‑1 (MQ=255)
                                                                                                  gTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                        >  4:244524/1‑114 (MQ=255)
                                                                                                                  cTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGGTGCGGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtg                     <  1:308993/149‑1 (MQ=255)
                                                                                                                   tGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                    >  5:50679/1‑149 (MQ=255)
                                                                                                                     cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                    <  6:318839/147‑1 (MQ=255)
                                                                                                                     cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                    <  1:48952/147‑1 (MQ=255)
                                                                                                                      tCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                    >  1:75609/1‑146 (MQ=255)
                                                                                                                        ggCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc               >  4:198916/1‑149 (MQ=255)
                                                                                                                         caaaggCAGCAGCCGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc               >  5:281749/5‑148 (MQ=255)
                                                                                                                          cTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc               >  3:183552/1‑147 (MQ=255)
                                                                                                                              gCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGt         <  6:50679/149‑1 (MQ=255)
                                                                                                                                     ctgctgCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCgg  <  6:3763/149‑1 (MQ=255)
                                                                                                                                      tgctgcCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTc    >  7:178879/1‑146 (MQ=255)
                                                                                                                                      tgctgcCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTc    <  8:178879/146‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                   
AAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCCTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGG  >  NC_002947/855947‑856228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑