Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	874,540	A→G	6.2%	E40G (GAA→GGA) ‡	PP_0759 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	874,540	0	A	G	6.2%	183.7 / 2.5	64	E40G (GAA→GGA) ‡	PP_0759	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

CGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGC  >  NC_002947/874399‑874687                                                                                                                                               |                                                                                                                                                     cGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTACTGTTGGCGTTCGACGCCaa                                                                                                                                               <  4:82478/149‑1 (MQ=255)       ggCCTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTa                                                                                                                                          >  1:147082/1‑148 (MQ=255)       ggCCTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTa                                                                                                                                          <  2:147082/148‑1 (MQ=255)        gCCTAGGATACGGCGCATGTGGATAGGGAGTGTGTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTa                                                                                                                                          >  1:46749/1‑148 (MQ=255)         ccTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACAt                                                                                                                                       <  1:237372/149‑1 (MQ=255)          cTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATg                                                                                                                                      <  4:331609/149‑1 (MQ=255)               aTACGGCGCATGTGGATAGGCAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATTTTCa                                                                                                                                  <  7:220861/148‑1 (MQ=255)                tACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAg                                                                                                                                 >  7:38999/1‑148 (MQ=255)                tACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGc                                                                                                                                <  7:207358/149‑1 (MQ=255)                 aCGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAAc                                                                                                                                                    <  1:332628/128‑1 (MQ=255)                 aCGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAAc                                                                                                                                                    >  2:332628/1‑128 (MQ=255)                    gcgcATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGc                                                                                                                            >  2:246556/1‑149 (MQ=255)                        aTGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTTCGACGCCAAc                                                                                                                                              <  5:249998/127‑1 (MQ=255)                        aTGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTTCGACGCCAAc                                                                                                                                              >  6:249998/1‑127 (MQ=255)                        aTGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACa                                                                                                                                        >  2:20956/1‑133 (MQ=255)                        aTGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACa                                                                                                                                        <  1:20956/133‑1 (MQ=255)                            ggATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGAcc                                                                                                                    >  7:243644/1‑149 (MQ=255)                                aGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                 <  8:243644/148‑1 (MQ=255)                                  ggAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                 <  6:306325/146‑1 (MQ=255)                                  ggAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                 >  5:306325/1‑146 (MQ=255)                                  ggAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAg                                                                                                              <  4:158372/149‑1 (MQ=255)                                     gtgt‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTAGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGt                                                                                                            >  5:109097/1‑148 (MQ=255)                                        t‑tttCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAAc                                                                                                        >  3:298476/1‑149 (MQ=255)                                        t‑tttCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAAc                                                                                                        >  4:107169/1‑149 (MQ=255)                                              aTGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                   >  8:12970/1‑149 (MQ=255)                                               tGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATg                                                                                                  >  2:176513/1‑149 (MQ=255)                                               tGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATg                                                                                                  >  4:284536/1‑149 (MQ=255)                                                 cGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGt                                                                                                 <  2:46749/148‑1 (MQ=255)                                                    aaGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑cgc                                                                                            >  8:186473/1‑149 (MQ=255)                                                     aGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑cgc                                                                                            <  2:285216/148‑1 (MQ=255)                                                       tCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑cgcg                                                                                           >  8:132167/1‑147 (MQ=255)                                                       tCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCa                                                                                         <  3:107169/149‑1 (MQ=255)                                                              tcatcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTa                                                                                   <  1:176513/148‑1 (MQ=255)                                                              tcatcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTa                                                                                   >  6:49963/1‑148 (MQ=255)                                                                atcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTAt                                                                                  >  2:98737/1‑147 (MQ=255)                                                                atcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATca                                                                                <  3:284536/149‑1 (MQ=255)                                                                       tgctgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGAcc                                                                         >  6:31656/1‑149 (MQ=255)                                                                               ccTGGCAGCCGGCCAGCAGGATGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTg                                                                  >  2:99108/2‑148 (MQ=255)                                                                                cTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGa                                                                 <  7:132167/148‑1 (MQ=255)                                                                                cTGGCAGCCGGCCAGCAGGATGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTg                                                                  >  5:57118/1‑147 (MQ=255)                                                                                     aTCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                   <  7:54227/110‑1 (MQ=255)                                                                                     aTCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                   >  8:54227/1‑110 (MQ=255)                                                                                      tCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATc                                                           >  6:118220/1‑148 (MQ=255)                                                                                        ctgctgGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGa                                                         <  4:298476/148‑1 (MQ=255)                                                                                           ctgGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGCCCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGc                                                       >  6:4616/1‑147 (MQ=255)                                                                                              gCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAggg                                                  <  5:49963/149‑1 (MQ=255)                                                                                              gCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                   <  5:31656/148‑1 (MQ=255)                                                                                                        aGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTAAGCATGCGGTCGACCGATTAGGTCAACCAGATGTA‑CGCGCGGGCGTATGAGCAGCCCGTGGGGGAAGCATCGAACAAGGGGGTGGTc                                           >  8:212078/1‑146 (MQ=255)                                                                                                          gCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACaa                                      <  7:301911/149‑1 (MQ=255)                                                                                                             gTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑c                                                                                              <  2:129167/90‑1 (MQ=255)                                                                                                             gTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑c                                                                                              >  1:129167/1‑90 (MQ=255)                                                                                                             gTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGAGCAGTCTTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTc                                   >  6:351265/1‑149 (MQ=255)                                                                                                              tGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTATAGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTc                                   <  1:98737/149‑1 (MQ=255)                                                                                                                  tACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAgcg                              >  6:93171/1‑149 (MQ=255)                                                                                                                   accaccAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATCAGGTCAACCAGATGTA‑cgcg                                                                                           <  4:230846/87‑1 (MQ=255)                                                                                                                       ccaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGAt                         <  6:109097/149‑1 (MQ=255)                                                                                                                           cggcggTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATc                                                                                 >  7:72677/1‑89 (MQ=255)                                                                                                                           cggcggTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATc                                                                                 <  8:72677/89‑1 (MQ=255)                                                                                                                               ggTGCTGTTGGCGTCGAACGCCAACAGTAGATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCCGTCGTATCAGAAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAACTCCAGCGCCGATGCCAAGCg                 >  1:5636/1‑149 (MQ=255)                                                                                                                                 tacTGTTGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgtg               >  6:326443/3‑149 (MQ=255)                                                                                                                                           gtagAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGcc                      >  4:6273/4‑132 (MQ=255)                                                                                                                                           gTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGcc                      <  3:6273/132‑1 (MQ=255)                                                                                                                                            tCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCAtt    <  5:93171/149‑1 (MQ=255)                                                                                                                                              gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGc  >  8:55805/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                     CGACTCGGCCTAGGATACGGCGCATGTGGATAGGGAGTGT‑TTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTA‑CGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGC  >  NC_002947/874399‑874687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑