Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 875,284 T→A 7.3% intergenic (+23/+23) PP_0759 → / ← PP_0760 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947875,2840TA 7.3% 154.9 / 2.7 55intergenic (+23/+23)PP_0759/PP_0760hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

GGCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTACC  >  NC_002947/875138‑875429
                                                                                                                                                   |                                                                                                                                                 
ggCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTACCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATgg                                                                                                                                                 >  3:164883/1‑149 (MQ=255)
  cTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCa                                                                                                                                               <  5:23403/149‑1 (MQ=255)
     ccGCAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGTTTATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAg                                                                                                                                              <  6:24974/147‑1 (MQ=255)
          ggTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGAATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCATGCAGccccagg                                                                                                                                       <  8:292906/149‑4 (MQ=255)
           gTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGcccccgga                                                                                                                                      <  4:33340/149‑4 (MQ=255)
             gCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGt                                                                                                                                    >  2:260245/1‑149 (MQ=255)
             gCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGt                                                                                                                                    >  2:282825/1‑149 (MQ=255)
                ccGCCTGAGTTCATGAGTACTCACCTGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGtttt                                                                                                                                 >  3:300562/1‑149 (MQ=255)
                ccGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGtttt                                                                                                                                 <  1:260245/149‑1 (MQ=255)
                 cGCCTGAGTTCATGAGTACGCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTc                                                                                                                                <  4:50954/149‑1 (MQ=255)
                  gCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTcc                                                                                                                               <  6:293719/149‑1 (MQ=255)
                   ccTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCGGGGGCTGCCATGCAGcccca                                                                                                                                         <  1:345722/138‑2 (MQ=255)
                   ccTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCGGGGGCTGCCATGCAGcccca                                                                                                                                         >  2:345722/1‑137 (MQ=255)
                    cTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGTACGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTccc                                                                                                                              >  1:263581/1‑149 (MQ=255)
                       aGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCtt                                                                                                                            <  5:1141/147‑1 (MQ=255)
                       aGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTa                                                                                                                          <  3:50456/149‑1 (MQ=255)
                         ttCATGAGTACTCACCTGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTAcc                                                                                                                        <  4:300562/149‑1 (MQ=255)
                            aTGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCaa                                                                                                                     >  4:316251/1‑149 (MQ=255)
                             tGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTACCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAAt                                                                                                                    <  4:164883/149‑1 (MQ=255)
                                 tACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCa                                                                                                                 <  5:63565/148‑1 (MQ=255)
                                      aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACt                                                                                                           >  2:19923/1‑149 (MQ=255)
                                        ccGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTgg                                                                                                         >  6:140978/1‑149 (MQ=255)
                                                   gAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGcc                                                                                              <  8:220018/149‑1 (MQ=255)
                                                      ccGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGaa                                                                                           >  7:34051/1‑149 (MQ=255)
                                                          aTTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGa                                                                                       >  8:188935/1‑149 (MQ=255)
                                                               ttCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGcc                                                                                  >  3:156039/1‑149 (MQ=255)
                                                               ttCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGc                                                                                   >  3:150974/1‑148 (MQ=255)
                                                                      cAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGAtt                                                                            <  5:140978/148‑1 (MQ=255)
                                                                            ggcgATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGt                                                                      <  2:263581/149‑1 (MQ=255)
                                                                                 ttCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACg                                                                   >  3:267040/1‑147 (MQ=255)
                                                                                    cGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGcc                                                                                              >  2:36397/1‑117 (MQ=255)
                                                                                    cGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGcc                                                                                              <  1:36397/117‑1 (MQ=255)
                                                                                     gAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAAc                                                             >  8:273442/1‑149 (MQ=255)
                                                                                          tGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGACCACGATTGCCAGTACGAAGAACGCCGt                                                        <  1:19923/149‑1 (MQ=255)
                                                                                           gATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGtt                                                       <  4:156039/149‑1 (MQ=255)
                                                                                            aTGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGtt                                                       <  4:150974/148‑1 (MQ=255)
                                                                                                       tCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcgg                                           <  7:133139/149‑1 (MQ=255)
                                                                                                       tCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATACACCCACAGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcgg                                           >  1:173878/1‑149 (MQ=255)
                                                                                                        cAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCACGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATAGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcgg                                           <  2:122823/148‑1 (MQ=255)
                                                                                                           gCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGaa                                       <  2:22595/149‑1 (MQ=255)
                                                                                                            cTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCAGTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTTCCGCCAGGCGGCGAAt                                      <  6:312317/149‑1 (MQ=255)
                                                                                                                 gccAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCg                                 >  3:220819/1‑149 (MQ=255)
                                                                                                                  ccAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGt                                >  8:79212/1‑149 (MQ=255)
                                                                                                                   cAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTc                               <  8:34051/149‑1 (MQ=255)
                                                                                                                             aTGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGtt                     >  3:226645/1‑149 (MQ=255)
                                                                                                                                ccTTAATCCTGGGGCGGCATGGAAGCCCCCCCGG‑TTTCCCTTTACCCAATCCTGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGt                  <  1:282825/148‑1 (MQ=255)
                                                                                                                                ccTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGt                  <  7:79212/149‑1 (MQ=255)
                                                                                                                                  tGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAgacaaact                >  3:103726/1‑142 (MQ=255)
                                                                                                                                  tGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCt                >  6:265659/1‑149 (MQ=255)
                                                                                                                                     ccgggggggCTGCCATGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGcc             >  3:49586/6‑149 (MQ=255)
                                                                                                                                      tCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcg            >  2:234421/1‑149 (MQ=255)
                                                                                                                                      tCCTGGGGCTGCATGGCAG‑CCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcgc           >  5:180429/1‑149 (MQ=255)
                                                                                                                                       ccTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcgc           >  5:147418/1‑149 (MQ=255)
                                                                                                                                            ggCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGt      <  3:316251/149‑1 (MQ=255)
                                                                                                                                                gCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTAcc  >  4:80523/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAG‑GCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAAGTTACC  >  NC_002947/875138‑875429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑