Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 124,743 G→A 8.3% Q125* (CAG→TAG)  PP_0119 ← zinc uptake regulation protein Zur

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947124,7430GA 8.3% 134.3 / 2.6 48Q125* (CAG→TAG) PP_0119zinc uptake regulation protein Zur
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCCAGCAGGAAGTCCAGCGCA  >  NC_002947/124597‑124885
                                                                                                                                                  |                                                                                                                                               
cGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGctgctctgct                                                                                                                                               <  4:289945/149‑1 (MQ=255)
    gACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTgctcgag                                                                                                                                           >  4:263257/1‑149 (MQ=255)
       cTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTgctcgagctc                                                                                                                                        >  6:66197/1‑149 (MQ=255)
           tCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCCGCTCGAGCTC‑GATg                                                                                                                                   <  6:204357/149‑1 (MQ=255)
                     tCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGc                                                                                                                         <  3:263257/149‑1 (MQ=255)
                       aGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCa                                                                                                                        <  5:66197/148‑1 (MQ=255)
                       aGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAg                                                                                                                       <  4:309280/149‑1 (MQ=255)
                           cGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGccc                                                                                                                   <  4:186771/149‑1 (MQ=255)
                           cGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGccc                                                                                                                   <  7:231097/149‑1 (MQ=255)
                                  cATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGAt                                                                                                            <  3:205940/149‑1 (MQ=255)
                                    tGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCa                                                                                                          <  1:25420/149‑1 (MQ=255)
                                     gCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAg                                                                                                         >  8:274526/1‑149 (MQ=255)
                                            tCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTACTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTgg                                                                                                  >  2:252334/1‑149 (MQ=255)
                                            tCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTACTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTgg                                                                                                  >  2:247607/1‑149 (MQ=255)
                                                  aGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGaa                                                                                                      <  5:55992/139‑1 (MQ=255)
                                                  aGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGaa                                                                                                      >  6:55992/1‑139 (MQ=255)
                                                   gTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTACTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGt                                                                                           <  1:252334/149‑1 (MQ=255)
                                                   gTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTACTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGt                                                                                           <  1:247607/149‑1 (MQ=255)
                                                      aCCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGctgctctgct                                                                                                                                               <  8:96435/95‑1 (MQ=255)
                                                              ggCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCg                                                                                >  2:234503/1‑149 (MQ=255)
                                                                 cGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCgggg                                                                             >  4:281224/1‑149 (MQ=255)
                                                                        ttCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGTCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGACTg                                                                       >  5:332744/1‑148 (MQ=255)
                                                                        ttCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTg                                                                       >  6:192676/1‑148 (MQ=255)
                                                                           cACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGc                                                                   >  3:127338/1‑149 (MQ=255)
                                                                                  tGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGCCAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATg                                                              >  8:131476/1‑147 (MQ=255)
                                                                                      tctcGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCg                                                        >  8:93199/1‑149 (MQ=255)
                                                                                      tctcGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCg                                                        >  5:178188/1‑149 (MQ=255)
                                                                                        tcGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGc                                                                         >  3:159576/1‑130 (MQ=255)
                                                                                        tcGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGc                                                                         <  4:159576/130‑1 (MQ=255)
                                                                                          gACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGtt                                                      <  7:274526/147‑1 (MQ=255)
                                                                                          gACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGtt                                                      >  3:196074/1‑147 (MQ=255)
                                                                                             cGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCATGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAgc                                                  >  6:300438/1‑148 (MQ=255)
                                                                                                 aaaCCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCTACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAgcgatg                                              <  2:5367/148‑1 (MQ=255)
                                                                                                  aaCCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGACTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAgcgatcc                                             >  7:166394/1‑146 (MQ=255)
                                                                                                          ccTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACTTGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCGGATGAAGGCGTTCAGcgatgcgaggcgatg                                    >  6:186599/1‑149 (MQ=255)
                                                                                                            tttGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTCGGATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGcgatgcgatgcgatg                                    >  1:81223/1‑148 (MQ=255)
                                                                                                                   cTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGTCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGACTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGcc                           <  6:332744/149‑1 (MQ=255)
                                                                                                                      ttGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGCCCttt                        >  8:131059/1‑149 (MQ=255)
                                                                                                                        gatgatGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGtt                                                      <  8:288735/117‑1 (MQ=255)
                                                                                                                        gatgatGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGtt                                                      >  7:288735/1‑117 (MQ=255)
                                                                                                                        gatgatGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGtttt                      >  3:129179/1‑149 (MQ=255)
                                                                                                                         atgatgGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTc                     <  3:281224/149‑1 (MQ=255)
                                                                                                                          tgatgGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTc                     <  7:93199/148‑1 (MQ=255)
                                                                                                                            atgGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCca                   <  8:167080/148‑1 (MQ=255)
                                                                                                                              ggCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCCCATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGGTTTCcag                  >  3:82493/1‑147 (MQ=255)
                                                                                                                                ccTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCa                                                    <  3:84025/111‑1 (MQ=255)
                                                                                                                                ccTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCa                                                    >  4:84025/1‑111 (MQ=255)
                                                                                                                                ccTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCCAGCAgg              <  4:127338/149‑1 (MQ=255)
                                                                                                                                 cTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCCAGCAgg              >  7:317218/1‑148 (MQ=255)
                                                                                                                                    gCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGcc                           <  3:207563/132‑1 (MQ=255)
                                                                                                                                    gCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGcc                           >  4:207563/1‑132 (MQ=255)
                                                                                                                                        aTGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCCAGCAGGAAGTcc        <  6:178188/147‑1 (MQ=255)
                                                                                                                                            tgctctgctcGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCCAGCAGGAAGTCCAGCGCa  >  4:10695/1‑149 (MQ=255)
                                                                                                                                              ctctgctcGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCcagc                 >  2:240038/1‑132 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGCCGACCTGATCCAGGCGAATCAGGGCGTCGCTCATGCCGCGCTCCGGCAGTTACCGCACAGGCCGACCACTTCCACGGTCTGGGTCTCGACCGTGAAACCCACGCCTTTGGCGCTGTTGATGATGGCCTCGCTGATGCTGCTCTGCTCGAGCTC‑GATGGCCACATGGCAGGCCCGACAGATCAGGAACTGGCCTTGGTGCACGTGCTCGGGGTGGCTGCAGCCGATGAAGGCGTTCAGCGATGCGATGCGATGTACCAGGCCGTTTTCCAGCAGGAAGTCCAGCGCA  >  NC_002947/124597‑124885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑