Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	895,198	G→C	13.3%	intergenic (+38/+26)	PP_0777 → / ← PP_0778	glutathione peroxidase/group 1 family glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	895,198	0	G	C	13.3%	118.9 / 10.7	45	intergenic (+38/+26)	PP_0777/PP_0778	glutathione peroxidase/group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base C (2/4);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

CGAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCA  >  NC_002947/895052‑895341                                                                                                                                                   |                                                                                                                                                cGAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGa                                                                                                                                                <  8:212256/148‑1 (MQ=255) cGAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGa                                                                                                                                                <  3:279762/148‑1 (MQ=255)  gagaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGt                                                                                                                                              >  1:154584/1‑149 (MQ=255)   agaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCTCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGAGATGTTTTGGCACCTGTGAGATCGAGTg                                                                                                                                             <  1:195074/149‑1 (MQ=255)    gaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGc                                                                                                                                            <  7:194548/149‑1 (MQ=255)                    aaGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTg                                                                                                                            >  6:73136/1‑149 (MQ=255)                      ggATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCGGGCCTGAAGAGGGCCTGACGTGCTGTTTTGGCACCTGTGAGATCGAGCGCCGCCCGCGCGGCACTCGa                                                                                                                          <  1:222203/149‑1 (MQ=38)                       gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                         <  7:75179/149‑1 (MQ=39)                       gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                         <  3:48612/149‑1 (MQ=39)                             cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                   <  2:154584/149‑1 (MQ=255)                             cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                   <  1:302013/149‑1 (MQ=255)                                gTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAggt                                                                                                                <  8:127970/149‑2 (MQ=38)                                           tttCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACAt                                                                                                     >  3:154286/1‑149 (MQ=255)                                               tcGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTgcg                                                                                                 <  5:73136/149‑1 (MQ=255)                                                     cgcACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAAc                                                                                           <  2:44464/149‑1 (MQ=255)                                                             gcCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGcc                                                                                   >  1:67198/1‑149 (MQ=255)                                                                ggATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtctc                                                                                                                      <  6:93510/111‑1 (MQ=37)                                                                ggATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtctc                                                                                                                      >  5:93510/1‑111 (MQ=37)                                                                    gACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCAc                                                                            >  4:328780/1‑149 (MQ=255)                                                                       ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                         >  8:274850/1‑149 (MQ=255)                                                                       ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                         >  2:171131/1‑149 (MQ=255)                                                                       ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                         >  4:28692/1‑149 (MQ=255)                                                                        cAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAgg                                                                        >  3:216871/1‑149 (MQ=255)                                                                             gtggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGcc                                                                   <  4:154286/149‑1 (MQ=255)                                                                              tggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCg                                                                  <  3:28692/149‑1 (MQ=255)                                                                                gtgCAGGCCATCGAGAACGCCCTGGCCTGAAGAGGGCCTGACGTGATGTTTTGGCAGCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGc                                                                <  8:75894/149‑1 (MQ=255)                                                                                 tgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGc                                                                <  8:75893/148‑1 (MQ=255)                                                                                  gCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCAt                                                              <  1:171131/149‑1 (MQ=255)                                                                                     ggCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgcg                                                           >  5:244597/1‑149 (MQ=255)                                                                                                          cTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAAc                                      >  2:238674/1‑149 (MQ=255)                                                                                                               gagTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTc                                 >  5:47330/1‑149 (MQ=255)                                                                                                                 gTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGa                               <  6:131286/149‑1 (MQ=255)                                                                                                                        aCGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCATGTTGCCGGCATGCGCTGTTCAGATACCCCTCTAACTGTTCGACAATc                          <  2:67198/147‑1 (MQ=255)                                                                                                                        aCGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGc                        >  6:29977/1‑149 (MQ=255)                                                                                                                                ttttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAg                 <  7:274850/148‑1 (MQ=255)                                                                                                                                ttttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGc                >  8:245106/1‑149 (MQ=255)                                                                                                                                  ttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGcc               <  1:238674/148‑1 (MQ=255)                                                                                                                                      cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggc          <  3:55110/149‑1 (MQ=255)                                                                                                                                      cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggc          >  6:312980/1‑149 (MQ=255)                                                                                                                                       aCCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                         <  5:253468/85‑1 (MQ=255)                                                                                                                                       aCCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                         >  6:253468/1‑85 (MQ=255)                                                                                                                                           gtgAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCACTCGAACTGTTCGACAATCGCCGGCCACCCCTGCCGGCTg     >  8:136950/1‑149 (MQ=38)                                                                                                                                              agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGTCCTGGCGGCTGGCa  >  6:10248/1‑149 (MQ=255)                                                                                                                                              agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGTCTGGCa  <  8:180877/149‑1 (MQ=255)                                                                                                                                              agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGc   <  6:47330/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                CGAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCA  >  NC_002947/895052‑895341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑