Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	895,201	T→C	16.3%	intergenic (+41/+23)	PP_0777 → / ← PP_0778	glutathione peroxidase/group 1 family glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	895,201	0	T	C	16.3%	89.5 / 11.6	43	intergenic (+41/+23)	PP_0777/PP_0778	glutathione peroxidase/group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base C (2/5);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-02

GAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCG  >  NC_002947/895053‑895349                                                                                                                                                     |                                                                                                                                                     gagaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGt                                                                                                                                                      >  1:154584/1‑149 (MQ=255)  agaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCTCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGAGATGTTTTGGCACCTGTGAGATCGAGTg                                                                                                                                                     <  1:195074/149‑1 (MQ=255)   gaAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGc                                                                                                                                                    <  7:194548/149‑1 (MQ=255)                   aaGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTg                                                                                                                                    >  6:73136/1‑149 (MQ=255)                     ggATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCGGGCCTGAAGAGGGCCTGACGTGCTGTTTTGGCACCTGTGAGATCGAGCGCCGCCCGCGCGGCACTCGa                                                                                                                                  <  1:222203/149‑1 (MQ=38)                      gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                                 <  7:75179/149‑1 (MQ=39)                      gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                                 <  3:48612/149‑1 (MQ=39)                            cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                           <  1:302013/149‑1 (MQ=255)                            cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                           <  2:154584/149‑1 (MQ=255)                               gTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAggt                                                                                                                        <  8:127970/149‑2 (MQ=38)                                          tttCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACAt                                                                                                             >  3:154286/1‑149 (MQ=255)                                              tcGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTgcg                                                                                                         <  5:73136/149‑1 (MQ=255)                                                    cgcACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAAc                                                                                                   <  2:44464/149‑1 (MQ=255)                                                            gcCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGcc                                                                                           >  1:67198/1‑149 (MQ=255)                                                               ggATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtctc                                                                                                                              >  5:93510/1‑111 (MQ=37)                                                               ggATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtctc                                                                                                                              <  6:93510/111‑1 (MQ=37)                                                                   gACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCAc                                                                                    >  4:328780/1‑149 (MQ=255)                                                                      ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 >  4:28692/1‑149 (MQ=255)                                                                      ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 >  8:274850/1‑149 (MQ=255)                                                                      ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 >  2:171131/1‑149 (MQ=255)                                                                       cAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAgg                                                                                >  3:216871/1‑149 (MQ=255)                                                                            gtggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGcc                                                                           <  4:154286/149‑1 (MQ=255)                                                                             tggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCg                                                                          <  3:28692/149‑1 (MQ=255)                                                                               gtgCAGGCCATCGAGAACGCCCTGGCCTGAAGAGGGCCTGACGTGATGTTTTGGCAGCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGc                                                                        <  8:75894/149‑1 (MQ=255)                                                                                tgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGc                                                                        <  8:75893/148‑1 (MQ=255)                                                                                 gCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCAt                                                                      <  1:171131/149‑1 (MQ=255)                                                                                    ggCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgcg                                                                   >  5:244597/1‑149 (MQ=255)                                                                                                         cTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAAc                                              >  2:238674/1‑149 (MQ=255)                                                                                                              gagTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTc                                         >  5:47330/1‑149 (MQ=255)                                                                                                                gTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGa                                       <  6:131286/149‑1 (MQ=255)                                                                                                                       aCGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCATGTTGCCGGCATGCGCTGTTCAGATACCCCTCTAACTGTTCGACAATc                                  <  2:67198/147‑1 (MQ=255)                                                                                                                       aCGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGc                                >  6:29977/1‑149 (MQ=255)                                                                                                                               ttttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAg                         <  7:274850/148‑1 (MQ=255)                                                                                                                               ttttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGc                        >  8:245106/1‑149 (MQ=255)                                                                                                                                 ttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGcc                       <  1:238674/148‑1 (MQ=255)                                                                                                                                     cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggc                  <  3:55110/149‑1 (MQ=255)                                                                                                                                     cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggc                  >  6:312980/1‑149 (MQ=255)                                                                                                                                      aCCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 <  5:253468/85‑1 (MQ=255)                                                                                                                                      aCCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                 >  6:253468/1‑85 (MQ=255)                                                                                                                                          gtgAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCACTCGAACTGTTCGACAATCGCCGGCCACCCCTGCCGGCTg             >  8:136950/1‑149 (MQ=38)                                                                                                                                             agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGTCCTGGCGGCTGGCa          >  6:10248/1‑149 (MQ=255)                                                                                                                                             agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGTCTGGCa          <  8:180877/149‑1 (MQ=255)                                                                                                                                             agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGc           <  6:47330/148‑1 (MQ=255)                                                                                                                                                    gTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTc   >  4:197762/1‑149 (MQ=255)                                                                                                                                                     tGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTcg  >  6:11358/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GAGAAGTTTCTGGTGGGCAAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCG  >  NC_002947/895053‑895349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑