Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	895,219	G→C	15.6%	intergenic (+59/+5)	PP_0777 → / ← PP_0778	glutathione peroxidase/group 1 family glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	895,219	0	G	C	15.6%	103.5 / 13.5	45	intergenic (+59/+5)	PP_0777/PP_0778	glutathione peroxidase/group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base C (2/5);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

AAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCGCGCATTCAGGC  >  NC_002947/895071‑895360                                                                                                                                                     |                                                                                                                                              aaGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTg                                                                                                                                               >  6:73136/1‑149 (MQ=255)   ggATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCGGGCCTGAAGAGGGCCTGACGTGCTGTTTTGGCACCTGTGAGATCGAGCGCCGCCCGCGCGGCACTCGa                                                                                                                                             <  1:222203/149‑1 (MQ=38)    gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                                            <  7:75179/149‑1 (MQ=39)    gATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAt                                                                                                                                            <  3:48612/149‑1 (MQ=39)          cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                                      <  1:302013/149‑1 (MQ=255)          cGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                                      <  2:154584/149‑1 (MQ=255)             gTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAggt                                                                                                                                   <  8:127970/149‑2 (MQ=38)                        tttCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACAt                                                                                                                        >  3:154286/1‑149 (MQ=255)                            tcGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTgcg                                                                                                                    <  5:73136/149‑1 (MQ=255)                                  cgcACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAAc                                                                                                              <  2:44464/149‑1 (MQ=255)                                          gcCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGcc                                                                                                      >  1:67198/1‑149 (MQ=255)                                             ggATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtctc                                                                                                                                         <  6:93510/111‑1 (MQ=37)                                             ggATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtctc                                                                                                                                         >  5:93510/1‑111 (MQ=37)                                                 gACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCAc                                                                                               >  4:328780/1‑149 (MQ=255)                                                    ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            >  2:171131/1‑149 (MQ=255)                                                    ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            >  8:274850/1‑149 (MQ=255)                                                    ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            >  4:28692/1‑149 (MQ=255)                                                     cAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAgg                                                                                           >  3:216871/1‑149 (MQ=255)                                                          gtggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGcc                                                                                      <  4:154286/149‑1 (MQ=255)                                                           tggtgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCg                                                                                     <  3:28692/149‑1 (MQ=255)                                                             gtgCAGGCCATCGAGAACGCCCTGGCCTGAAGAGGGCCTGACGTGATGTTTTGGCAGCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGc                                                                                   <  8:75894/149‑1 (MQ=255)                                                              tgCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGc                                                                                   <  8:75893/148‑1 (MQ=255)                                                               gCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCAt                                                                                 <  1:171131/149‑1 (MQ=255)                                                                  ggCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATgcg                                                                              >  5:244597/1‑149 (MQ=255)                                                                                       cTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAAc                                                         >  2:238674/1‑149 (MQ=255)                                                                                            gagTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTc                                                    >  5:47330/1‑149 (MQ=255)                                                                                              gTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGa                                                  <  6:131286/149‑1 (MQ=255)                                                                                                     aCGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCATGTTGCCGGCATGCGCTGTTCAGATACCCCTCTAACTGTTCGACAATc                                             <  2:67198/147‑1 (MQ=255)                                                                                                     aCGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGc                                           >  6:29977/1‑149 (MQ=255)                                                                                                             ttttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAg                                    <  7:274850/148‑1 (MQ=255)                                                                                                             ttttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGc                                   >  8:245106/1‑149 (MQ=255)                                                                                                               ttGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGcc                                  <  1:238674/148‑1 (MQ=255)                                                                                                                   cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggc                             >  6:312980/1‑149 (MQ=255)                                                                                                                   cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggc                             <  3:55110/149‑1 (MQ=255)                                                                                                                    aCCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            >  6:253468/1‑85 (MQ=255)                                                                                                                    aCCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            <  5:253468/85‑1 (MQ=255)                                                                                                                        gtgAGATCCAGCGCCGCCCGCGCGGCACTCGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCACTCGAACTGTTCGACAATCGCCGGCCACCCCTGCCGGCTg                        >  8:136950/1‑149 (MQ=38)                                                                                                                           agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGTCCTGGCGGCTGGCa                     >  6:10248/1‑149 (MQ=255)                                                                                                                           agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGTCTGGCa                     <  8:180877/149‑1 (MQ=255)                                                                                                                           agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGc                      <  6:47330/148‑1 (MQ=255)                                                                                                                                  gTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTc              >  4:197762/1‑149 (MQ=255)                                                                                                                                   tGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTcg             >  6:11358/1‑149 (MQ=255)                                                                                                                                     ccGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTcgc            <  8:208735/148‑1 (MQ=255)                                                                                                                                      cgcgcgGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCgcgc          <  3:328780/149‑1 (MQ=255)                                                                                                                                         gcgggcggCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCGCGCAtt       >  1:36634/1‑149 (MQ=255)                                                                                                                                              cggcgCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGTCCTGGCGGCTGGCATGCTGTCGCGCATTCAGgc  <  5:10248/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                              AAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTTGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCGCGCATTCAGGC  >  NC_002947/895071‑895360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑