| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 924,320 | C→G | 12.3% | A291A (GCG→GCC) ‡ | PP_0804 ← | protein secretion ABC transporter permease/ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 924,320 | 0 | C | G | 12.3% | 144.8 / 13.7 | 57 | A291A (GCG→GCC) ‡ | PP_0804 | protein secretion ABC transporter permease/ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (27/23); new base G (4/3); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01 | |||||||||||
GCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTT > NC_002947/924174‑924466 | gCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGAGCAGCGGGATGAACACCAGTACGCCGCCGATGAGCCAGAACACGATCAGGAACAGCTGGAAGAACGGCAGGTGGGCCA‑GCAGCGTcgc < 6:102527/149‑1 (MQ=255)gCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTcgc > 6:187373/1‑149 (MQ=255)gCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTc < 8:120963/147‑1 (MQ=255)gCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTc > 7:120963/1‑147 (MQ=255) cAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCgg < 7:309468/149‑1 (MQ=255) ccTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCa < 5:187373/149‑1 (MQ=255) gcaagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTcgcg > 5:164737/1‑149 (MQ=255) gcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATc > 2:320810/1‑149 (MQ=255) gcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGAAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATc > 2:41785/1‑149 (MQ=255) cccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGCACGGCAGGTCGGCCC‑GCACCGCGCCGGTGCgggc > 6:98949/1‑125 (MQ=255) cccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGCGACGGTGCTggc < 5:98949/126‑2 (MQ=255) ccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGACA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATcacggg > 4:314249/1‑144 (MQ=255) aCCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTcgcg > 2:49283/1‑149 (MQ=255) cATTGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGAt < 2:98065/132‑1 (MQ=255) cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGCACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCATGCACCGTCGCGGTGCTGGACGTGATc < 1:178509/122‑1 (MQ=255) cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAg > 4:234081/1‑123 (MQ=255) cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAg < 3:234081/123‑1 (MQ=255) cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGAt > 1:98065/1‑132 (MQ=255) agcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGAt < 4:63504/149‑1 (MQ=255) gACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACg < 6:164737/149‑1 (MQ=255) gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgaga < 3:87540/134‑1 (MQ=255) gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgaga > 4:87540/1‑134 (MQ=255) cacCAGTACGGCGCCGAGCAGCCCGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGGGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg < 6:239825/149‑1 (MQ=255) aGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGAt < 3:283211/125‑1 (MQ=255) aGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGAt > 4:283211/1‑125 (MQ=255) gccgccGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCAcc > 6:327240/1‑148 (MQ=255) cgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGtt < 4:65561/148‑1 (MQ=255) cgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGtt > 3:65561/1‑148 (MQ=255) cgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCGGGACGGGGTGACGTCGCGGGTCGACACCAGCTCGCCCAACTGCGAGCAGAACGCGCCGGGGGGCGTGGGCCGCACCGAGTTg > 4:330699/1‑149 (MQ=255) aGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgca < 1:320810/148‑1 (MQ=255) aGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGACCCGGTGGACTAGCGCCGCACCGAGTTgcgcag > 4:88202/1‑149 (MQ=255) gcaaGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcagg < 6:131370/146‑1 (MQ=255) cAGAACACGAACAGGAACAGCAGGAAGCACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGAGCAGGGCGCGGAACGCCTCCAGCACGCGCCACTGCGAGATGAACGCGCCGGGGGACGTGGGCCGCCACGCGATgcgcagccg > 7:295037/1‑149 (MQ=255) aGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcg > 5:292818/1‑148 (MQ=255) gAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgca < 1:49283/149‑1 (MQ=255) acacGAACAGGACCAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGGGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGcgcaggcgcagg < 3:330699/149‑1 (MQ=255) gaacaggaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg > 7:144517/1‑119 (MQ=255) gaacaggaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg < 8:144517/119‑1 (MQ=255) gaacaggaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGCACGTGATCAGGTCGCGGATCGACTCCAGCTCCCGCAACAGCGAGATGAACGAGCCGGTGGACTTGCGCCGCACCGAGTTGCGCAGGCGCAGCGCAt > 2:257949/1‑149 (MQ=255) acaggaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCCTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTAGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATgg > 4:101480/1‑149 (MQ=255) gaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag > 4:53759/1‑138 (MQ=255) gaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATg < 8:311040/144‑1 (MQ=255) gaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATg > 7:311040/1‑144 (MQ=255) gaacagCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTCGGCCGCACCGAGTTgcgcaggcgcag < 3:53759/138‑1 (MQ=255) agcagGAAGAACGGCAGGTCGGCCA‑GCACCGGCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc < 3:88202/149‑1 (MQ=255) agGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAg > 6:285854/1‑149 (MQ=255) gaagaaCGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTggg < 6:101956/105‑1 (MQ=255) gaagaaCGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTggg > 5:101956/1‑105 (MQ=255) gaagaaCGGCAGGTCGGCCA‑GCACCGTCGCGGGGGGGGGCGTGGTGAGGTGGCGGATCGACTCCAGCACGCGCAACTGCGAGATGGACGCGCCGGGGGGCGTGGGGCGCCCCGCGTTTCGCAGGCGCCGGGGCTGGCCGGAGACCACGt > 6:41731/1‑149 (MQ=255) gaaCGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc > 1:226725/1‑141 (MQ=255) gaaCGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc < 2:226725/141‑1 (MQ=255) cGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCa < 5:285854/149‑1 (MQ=255) gtccA‑GCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAg < 3:248099/125‑1 (MQ=255) gtccA‑GCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAg > 4:248099/3‑127 (MQ=255) gtccA‑GCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGt > 6:143832/3‑149 (MQ=255) ccA‑GCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGcc < 6:270208/121‑1 (MQ=255) ccA‑GCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGcc > 5:270208/1‑121 (MQ=255) cA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCgg > 5:290548/1‑149 (MQ=255) gTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCCCCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCtt > 3:185432/1‑149 (MQ=255) gTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag < 4:36292/104‑1 (MQ=255) gTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag > 3:36292/1‑104 (MQ=255) | GCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCA‑GCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTT > NC_002947/924174‑924466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |