| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,149 | A→G | 14.2% | intergenic (+103/‑210) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,149 | 0 | A | G | 14.2% | 121.4 / 9.0 | 53 | intergenic (+103/‑210) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (22/20); new base G (3/4); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATT > NC_002947/955003‑955294 | aCAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACaa < 8:284700/147‑1 (MQ=255)aCAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACaa > 7:284700/1‑147 (MQ=255)aCAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAgg > 4:266175/1‑149 (MQ=255) ccAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTcc > 7:219986/1‑149 (MQ=255) aGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCt < 4:442/149‑1 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGc < 1:46782/147‑1 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 6:342508/149‑1 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 5:103400/149‑1 (MQ=255) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg < 2:25950/149‑1 (MQ=37) tgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑c < 5:283484/149‑1 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑cc < 5:135980/145‑1 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑cc > 6:135980/1‑145 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCcg < 3:266175/147‑1 (MQ=255) cTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCAACAAAAAGGTTCCTGTAGGAGCGGGTTTA‑ACCGCGAagag > 6:127291/1‑149 (MQ=39) acgcacgcTCACCCTGCTGCGTGTTGCACGAAAACCCCGGCCCCTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTGCGC‑GGGTAAACCCGCTCCTACACGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGc < 8:219986/149‑1 (MQ=37) acgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAg > 5:252928/1‑148 (MQ=255) gcTGAAACTGCTGCCTGTTTCACGTAAACCCCGGCCACTGG‑GCCGGGG‑TTTTTTTTTGGGGGTTTTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTGCTACAAGGGTCCTTTAGGCGGGGGTTTACCCCGCGAAGAGGCCAGca < 4:285913/149‑1 (MQ=37) tGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGGGAAGAGGCCAGca > 8:237616/1‑146 (MQ=255) ccTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGGTAAACCC‑CGGCGTCAACGGTGCTGTTGGGGGGGGGTTT‑ACCGCGGAGGGGGCCGCCCAGgaaaataaaaa > 3:119694/1‑149 (MQ=255) ccTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTTAACCCGCACCGTCAAGGTTACGGTAAGAGCGGGGTTT‑ACCGCGAAGCCGCCAGGACAGaaaaaaaaaa > 6:85887/1‑148 (MQ=255) ccTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTTCAAGGTTACTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGaaaaataaaaa > 1:65502/1‑149 (MQ=255) tGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGt < 4:335190/112‑1 (MQ=21) tGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGt > 3:335190/1‑112 (MQ=21) aaaaaCCCCGG‑CACAGTGGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCGCCGACAAGGTTACGGTGGGGGCGGGGTTG‑ACCGCGCAGAGGCCCGCACAGCAAAATAAAAATAACTGGttt > 4:239102/2‑149 (MQ=25) aaCCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAAACCCCAACTAAAAGGTTACTGTATGAGCGGGTTTA‑ACCGCGAAGAGGCCAGCACAGAAAAATAAAAAAAACAGAtttta > 6:125696/1‑147 (MQ=255) aaCCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTAGC‑GGGTTAAACCCCAACAAAAAGGGTACGGGATGAGCCGGTTTT‑ACCGCCAAGAGGGCACCACAGAAAAATAAAAATAAATGAattta > 6:186162/1‑147 (MQ=255) aaCCCCGG‑CACAGTGGCCGGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTATTCGC‑GGGTAAACCCACAACAAAAAAGTTACAGTAGGAACCGGTTTA‑ACCCCCAAGCAGCCAAAACACAAAAAAAAAAAAAACTGAattt > 6:275271/1‑148 (MQ=255) ccccGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTCCAGGAACCTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGc < 5:325392/115‑1 (MQ=18) cGGCCACTGT‑GCGGGGG‑TTTTTTTTTGGGGTTATTTTTCGTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACATGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAgg < 4:256942/111‑1 (MQ=25) tgt‑gCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTACTACAAGGTTACTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATAACTGATTTTCAAAAAATAGAtat > 1:12795/1‑147 (MQ=255) tttttttttGGGTTTATTTTTCCTGTGCCGGCCTATTCGC‑GGTAAAACCCGCTCATAAAATTTTACTTTAGTAGCGTTTTAA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc < 3:185613/148‑1 (MQ=255) tGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTAAAAGGTTCCTTTAGGAGCGGTTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaaa < 7:237616/148‑1 (MQ=255) gggggTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAAc < 6:252928/148‑1 (MQ=255) tATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCaaaaaa < 5:271721/118‑1 (MQ=255) tgCCGGCCTCTTCGC‑GGTTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTa < 2:12795/148‑1 (MQ=255) tgCCGGCCTATTCGC‑TGTTAAACACTATCATAAAAGTTTCCTTTAGGAGCTTGTTTA‑ACCGCGAAGAGGACAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTa < 5:275271/148‑1 (MQ=255) ccGGCCTCTTCGC‑GGTAAAACCCGATCAAAAAAGTTACCTTTAGTAGGGGTTTAA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa < 5:186162/148‑1 (MQ=255) tCGG‑GGGTAAACCCGCTCCAACATGTTTCCTTTATGTGCGGTTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTg < 2:307597/148‑1 (MQ=255) gTAAACCCGCTCCTACAAGGTTCCTTTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGc < 5:127291/149‑1 (MQ=255) gTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg > 7:14301/1‑148 (MQ=255) aaCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACt < 8:14301/149‑1 (MQ=255) aCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGc > 4:91699/1‑132 (MQ=255) aCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGc < 3:91699/132‑1 (MQ=255) cccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt > 5:323248/1‑149 (MQ=37) cccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt > 1:197576/1‑149 (MQ=255) ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCaaaaaa < 7:28453/84‑1 (MQ=17) ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCaaaaaa > 8:28453/1‑84 (MQ=17) ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc > 2:237664/1‑149 (MQ=255) gCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt > 5:309989/1‑149 (MQ=255) cTCCTACATGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc < 5:85887/149‑1 (MQ=255) tCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc < 6:337757/122‑1 (MQ=255) tCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc > 5:337757/1‑122 (MQ=255) tCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATc < 6:237122/129‑1 (MQ=255) tCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATc > 5:237122/1‑129 (MQ=255) tCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCAGCACTAtctact > 8:294176/1‑149 (MQ=255) aaGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt > 1:236839/1‑147 (MQ=255) | ACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGC‑GGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATT > NC_002947/955003‑955294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |