Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 127,121 C→G 7.9% intergenic (‑31/+39) thrB ← / ← PP_0122 homoserine kinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947127,1210CG 7.9% 225.6 / 7.4 76intergenic (‑31/+39)thrB/PP_0122homoserine kinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/35);  new base G (2/4);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

GAAATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCTTGCCGTTT  >  NC_002947/126974‑127268
                                                                                                                                                   |                                                                                                                                                   
gaaATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTccgcc                                                                                                                                                    >  5:225824/1‑149 (MQ=255)
  aaTTGCTGTTCTCGGTACCGGCGGCAATGCCATGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCgg                                                                                                                                                  >  1:205476/1‑149 (MQ=255)
    ttGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGa                                                                                                                                                <  4:277903/149‑1 (MQ=255)
          ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGcc                                                                                                                                          >  1:150534/1‑149 (MQ=255)
          ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGcc                                                                                                                                          <  2:205476/149‑1 (MQ=255)
          ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCCGGCGGAAGTGc                                                                                                                                           <  2:166729/148‑1 (MQ=255)
            ctcGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCCGGCGGAAGTGccc                                                                                                                                         <  7:193260/148‑2 (MQ=255)
                   ccGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCATCTCGTACGGCGCCATAAAGGTTTCCAGCTCAGTCCGTGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGACGGGAAGTGCCGGGTTGAt                                                                                                                                  <  1:47547/148‑1 (MQ=255)
                   ccGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAt                                                                                                                                  <  2:150534/148‑1 (MQ=255)
                    cggcggCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAtgt                                                                                                                                >  8:314016/1‑149 (MQ=255)
                    cggcggCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAtgt                                                                                                                                <  6:225824/149‑1 (MQ=255)
                       cggcAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGtt                                                                                                                                     >  7:314943/1‑141 (MQ=255)
                       cggcAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGtt                                                                                                                                     <  8:314943/141‑1 (MQ=255)
                             tGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTgc                                                                                                                       <  6:50037/149‑1 (MQ=255)
                             tGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTgc                                                                                                                       <  4:249217/149‑1 (MQ=255)
                                  tGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGt                                                                                                                   >  1:340754/1‑148 (MQ=255)
                                   ggAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGt                                                                                                                   >  5:134146/1‑147 (MQ=255)
                                    gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTc                                                                                                                  <  4:70408/147‑1 (MQ=255)
                                             cAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACCGGCACTTCCCGGCGGAAGTGcccgtatg                                                                                                                                    >  6:306225/1‑114 (MQ=38)
                                             cAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACCGGCACTTCCCGGCGGAAGTGcccgtatg                                                                                                                                    <  5:306225/120‑7 (MQ=38)
                                               gACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTc                                                                                                      >  3:148041/1‑148 (MQ=255)
                                                  ggCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCg                                                                                                     >  8:248484/1‑146 (MQ=255)
                                                   gCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCg                                                                                                     >  8:24286/1‑145 (MQ=255)
                                                   gCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCg                                                                                                     >  6:41059/1‑145 (MQ=255)
                                                    cccAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTAc                                                                                                           <  5:64838/138‑1 (MQ=255)
                                                    cccAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTAc                                                                                                           >  6:64838/1‑138 (MQ=255)
                                                    cccAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCg                                                                                                     >  8:5275/1‑144 (MQ=255)
                                                     ccAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATc                                                                                               >  1:212908/1‑149 (MQ=255)
                                                       aGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCtt                                                                                             <  6:134146/149‑1 (MQ=255)
                                                        gCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTc                                                                                            <  4:148041/149‑1 (MQ=255)
                                                           cGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCgg                                                                                                                                                  <  1:170312/92‑1 (MQ=255)
                                                           cGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCgg                                                                                                                                                  >  2:170312/1‑92 (MQ=255)
                                                            gTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCa                                                                                          <  7:248484/147‑1 (MQ=255)
                                                            gTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAgg                                                                                        <  2:208426/149‑1 (MQ=255)
                                                            gTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAg                                                                                         >  7:323522/1‑148 (MQ=255)
                                                             tACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTAc                                                                                                           <  6:128144/129‑1 (MQ=255)
                                                             tACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTAc                                                                                                           >  5:128144/1‑129 (MQ=255)
                                                             tACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGa                                                                                       <  1:27648/149‑1 (MQ=255)
                                                                ggCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACg                                                                                     >  2:10793/1‑148 (MQ=255)
                                                                   gcCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAAt                                                                                 <  3:234720/149‑1 (MQ=255)
                                                                      aGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAg                                                                              >  3:216887/1‑149 (MQ=255)
                                                                       gAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGc                                                                             <  2:290304/149‑1 (MQ=255)
                                                                        aaaGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCa                                                                            >  7:125701/1‑149 (MQ=255)
                                                                             tttCCATCTCATGCCGGGTCAGGGGGGTGAAGACTGACATTATGAAAAATTGCCCATACGGGCACTTGCTCCGTTAAGTTCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTGCAGGACGGa                                                                                   <  8:266967/137‑1 (MQ=255)
                                                                             tttCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTcc                                                                       >  4:48699/1‑149 (MQ=255)
                                                                             tttCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTcc                                                                       <  7:5275/149‑1 (MQ=255)
                                                                             tttCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAACTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACgca                                                                                   >  7:266967/1‑135 (MQ=255)
                                                                                ccAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCgg                                                                     <  7:24286/148‑1 (MQ=255)
                                                                                  aGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTg                                                                  <  2:212908/149‑1 (MQ=255)
                                                                                    cTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGt                                                                <  5:41059/149‑1 (MQ=255)
                                                                                    cTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGt                                                                >  2:60944/1‑149 (MQ=255)
                                                                                    cTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGt                                                                <  8:280968/149‑1 (MQ=255)
                                                                                       aGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTc                                                               <  1:60944/147‑1 (MQ=255)
                                                                                       aGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCgg                                                             <  8:125701/149‑1 (MQ=255)
                                                                                         gCCGTGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGAt                                                           <  8:323522/149‑1 (MQ=255)
                                                                                         gCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGAt                                                           >  4:96671/1‑149 (MQ=255)
                                                                                            gggTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCg                                                         >  3:212721/1‑148 (MQ=255)
                                                                                              gTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                                      <  6:175305/149‑1 (MQ=255)
                                                                                                accccaggTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATg                                                     <  1:294703/142‑1 (MQ=255)
                                                                                                   gggggTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAAt                                                 >  6:143678/1‑149 (MQ=255)
                                                                                                   gggggTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAAt                                                 >  2:272221/1‑149 (MQ=255)
                                                                                                                 aCATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCa                                   >  7:68225/1‑149 (MQ=255)
                                                                                                                 aCATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCa                                   >  1:331585/1‑149 (MQ=255)
                                                                                                                    tgatgaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGc                                                                             >  2:259462/1‑104 (MQ=255)
                                                                                                                      atgaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgc                              >  5:283662/1‑149 (MQ=255)
                                                                                                                      atgaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgc                              <  2:55300/149‑1 (MQ=255)
                                                                                                                       tgaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgcg                             >  1:173946/1‑149 (MQ=255)
                                                                                                                        gaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                                      >  2:235054/1‑123 (MQ=255)
                                                                                                                        gaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                                      <  1:235054/123‑1 (MQ=255)
                                                                                                                          aaaaTTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGcgcg                           >  3:185426/1‑148 (MQ=255)
                                                                                                                                 cccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGa                    <  7:89750/148‑1 (MQ=255)
                                                                                                                                 cccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGa                    >  4:95085/1‑148 (MQ=255)
                                                                                                                                    aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAAt                <  2:340754/149‑1 (MQ=255)
                                                                                                                                       cGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGACCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATaa              <  1:272221/148‑1 (MQ=255)
                                                                                                                                        gggCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAgg            <  6:283662/149‑1 (MQ=255)
                                                                                                                                         ggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGcc                                    <  2:215912/124‑1 (MQ=255)
                                                                                                                                         ggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGcc                                    >  1:215912/1‑124 (MQ=255)
                                                                                                                                                 cgccgGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCTTGCCGtt   >  5:66683/1‑149 (MQ=255)
                                                                                                                                                  gccgGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCTTGCCGttt  >  6:175322/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAAATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCTTGCCGTTT  >  NC_002947/126974‑127268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑