Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 961,710 G→C 7.0% H235Q (CAC→CAG)  selD ← selenide, water dikinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947961,7100GC 7.0% 176.3 / 3.3 57H235Q (CAC→CAG) selDselenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base G (23/30);  new base C (2/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCG  >  NC_002947/961564‑961854
                                                                                                                                                  |                                                                                                                                                
aTCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTg                                                                                                                                                >  3:305684/1‑149 (MQ=255)
 tCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTgg                                                                                                                                               >  8:192993/1‑149 (MQ=255)
        cccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGc                                                                                                                                         <  7:192993/148‑1 (MQ=255)
         ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGa                                                                                                                                           <  8:42409/145‑1 (MQ=255)
         ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGa                                                                                                                                           >  7:42409/1‑145 (MQ=255)
         ccGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCAGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAg                                                                                                                                       >  6:338079/1‑149 (MQ=255)
          cGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATCGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAgg                                                                                                                                      <  1:286383/149‑1 (MQ=255)
           gtcgCTGGCGTCGTTGCGCCGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCTACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                     <  8:214915/146‑1 (MQ=255)
           gTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGc                                                                                                                                     <  8:213057/149‑1 (MQ=255)
            tAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCTGCTCGGCCACCTGGTCGAGCTGGcc                                                                                                                                    <  5:22701/149‑1 (MQ=255)
             aGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGcc                                                                                                                                    >  5:198798/1‑148 (MQ=255)
               cTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGaa                                                                                                                                 <  7:115374/149‑1 (MQ=255)
                tGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAg                                                                                                                                >  7:10824/1‑149 (MQ=255)
                   cGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCGCGCCCCGGTGGCCGAGGAGGCGGAAGCCg                                                                                                                             >  8:296067/1‑149 (MQ=255)
                    gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCgg                                                                                                                            >  1:17169/1‑149 (MQ=255)
                    gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                             <  6:198798/148‑1 (MQ=255)
                    gTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCg                                                                                                                             <  7:196281/148‑1 (MQ=255)
                     tAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGt                                                                                                                           <  7:36124/149‑1 (MQ=255)
                       gTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGa                                                                                                                         <  2:17169/149‑1 (MQ=255)
                                ggTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCa                                                                                                                >  4:51423/1‑149 (MQ=255)
                                 gTTCCACCGGGGATGCAGCCTTCAGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCAt                                                                                                               <  5:338079/149‑1 (MQ=255)
                                  ttCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTAGGTCATg                                                                                                              >  1:266586/1‑149 (MQ=255)
                                  ttCCACCGGGGATGCAGCCTTCGGCCAGGAAATGGGCGACTCTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATg                                                                                                              <  8:149952/149‑1 (MQ=255)
                                      aCCGGGG‑TGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCtt                                                                                                         >  2:64213/1‑149 (MQ=255)
                                                cAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCAGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTc                                                                                                <  3:32425/149‑1 (MQ=255)
                                                    cTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGt                                                                                            <  2:266586/149‑1 (MQ=255)
                                                         gCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGcc                                                                                       <  8:10824/149‑1 (MQ=255)
                                                          ccAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGcc                                                                                       >  5:80926/1‑148 (MQ=255)
                                                            aGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCCGCCAGCTCGACCAGGTGGCCGCGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGaa                                                                                    >  5:203824/1‑149 (MQ=255)
                                                                 aTGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCgg                                                                                <  3:321171/148‑1 (MQ=255)
                                                                 aTGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGc                                                                               >  6:27029/1‑149 (MQ=255)
                                                                  tGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGAGGCt                                                                              >  5:90588/1‑149 (MQ=255)
                                                                     tCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGcc                                                                           <  5:27029/149‑1 (MQ=255)
                                                                       gtcagtAGGCTGGCGTGGCACGGGGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCgg                                                                         <  4:158843/144‑1 (MQ=255)
                                                                            tAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCgtg                                                                    >  5:211448/1‑149 (MQ=255)
                                                                               gcaggcGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTg                                                                 <  4:305684/149‑1 (MQ=255)
                                                                                      tGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGc                                                          <  1:64213/149‑1 (MQ=255)
                                                                                        gCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGca                                                         <  6:295259/148‑1 (MQ=255)
                                                                                             gcggcgTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCCGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGGTGAGGGTGccgatc                                                     >  8:133361/1‑142 (MQ=255)
                                                                                                     gTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACTGCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                            <  7:231365/148‑1 (MQ=255)
                                                                                                     gTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGa                                            <  6:211448/148‑1 (MQ=255)
                                                                                                               gcgGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                 <  3:51423/149‑1 (MQ=255)
                                                                                                                cgGTGAGGCCGCTGCCTTCGGCCAGCGCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTgg                                <  7:133361/149‑1 (MQ=255)
                                                                                                                  gTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTc                              <  1:187840/149‑1 (MQ=255)
                                                                                                                     aGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                 <  5:126956/143‑1 (MQ=255)
                                                                                                                     aGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTg                                 >  6:126956/1‑143 (MQ=255)
                                                                                                                     aGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCt                            <  6:80926/148‑1 (MQ=255)
                                                                                                                         cGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAACCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTAGGTCCTGcaca                       >  3:288239/1‑149 (MQ=255)
                                                                                                                                   ggCCTGCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTgg             >  1:228160/1‑149 (MQ=255)
                                                                                                                                    gCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGc            >  1:23554/1‑149 (MQ=255)
                                                                                                                                     ccAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACTCGCAGCTTGGcc           >  7:248214/1‑149 (MQ=255)
                                                                                                                                      ctgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACg                     <  5:178451/136‑1 (MQ=255)
                                                                                                                                      ctgCTCGGCCACCTGGTCGAGCTGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACg                     >  6:178451/3‑138 (MQ=255)
                                                                                                                                         cTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCcttct       >  5:168199/1‑149 (MQ=255)
                                                                                                                                            gACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCtttt    <  6:168199/149‑1 (MQ=255)
                                                                                                                                             aCCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTc   <  6:203824/149‑1 (MQ=255)
                                                                                                                                              ccAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCg  >  5:325453/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATCTTGTGCCCGTAGCTGTCGTAGTTGCGCAGGGTTCCACCGGGGATGCAGCCTTCGGCCAGGTAATGGTCGACACTAGGCAGGCGTGGCACGGCGGCGTAGTTCAGGTGCGCGGTGAGGCCGCTGCCTTCGGCCAGCTCGACCAGGTGGCCGAGCAGGCCGAAGCCGGTGACGTCGGTCATGGCCTTGACCCCGTCCAGTTTGCCGAAGCGGCTGCCGGGCGTGTTGAGGGTGCACATCCAGTCGCGAGCCAGGCCTTGGTCCTGCACACGCAGCTTGGCCTTCTTTTCG  >  NC_002947/961564‑961854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑