Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	983,587	T→A	8.0%	intergenic (+45/‑44)	iscX → / → ndk	cysteine desulfurase activity regulator/nucleoside diphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	983,587	0	T	A	8.0%	168.6 / 3.5	58	intergenic (+45/‑44)	iscX/ndk	cysteine desulfurase activity regulator/nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (31/22);  new base A (3/2);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGTGCTGGCATTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGCCGGCCTGAAAATCGT  >  NC_002947/983446‑983734                                                                                                                                               |                                                                                                                                                    ggTGCTGGCATTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCgg                                                                                                                                                <  7:198376/147‑1 (MQ=255)  gTGCTGGCATTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGtt                                                                                                                                             >  2:235325/1‑149 (MQ=255)   tGCTGGCACTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGAATTATACGCGGGttc                                                                                                                                            <  7:251140/149‑2 (MQ=255)     cTGGCATTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTaa                                                                                                                                          <  6:196906/149‑1 (MQ=255)        gCATTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGAATTATACGCGGGttccgg                                                                                                                                         <  4:252489/147‑5 (MQ=255)          aTTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAAttttt                                                                                                                                     >  3:152646/1‑149 (MQ=255)           ttGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTc                                                                                                                                    <  1:235325/149‑1 (MQ=255)           ttGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTc                                                                                                                                    <  2:11886/149‑1 (MQ=255)              ccAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCTGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACATGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGt                                                                                                                                  <  7:100572/148‑1 (MQ=255)                       aGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCata                                                                                                                        >  1:324710/1‑149 (MQ=255)                       aGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCata                                                                                                                        >  3:312202/1‑149 (MQ=255)                        gCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCatat                                                                                                                       >  7:189007/1‑149 (MQ=255)                          gacgaTCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTc                                                                                                                     <  6:122686/149‑1 (MQ=255)                            cgaTCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTg                                                                                                                   >  7:105174/1‑149 (MQ=255)                              aTCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGa                                                                                                                 <  5:137180/149‑1 (MQ=255)                                         ggcggTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATgg                                                                                                      <  4:152646/149‑1 (MQ=255)                                         ggcggTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATgg                                                                                                      <  8:105174/149‑1 (MQ=255)                                                   aaGTTCTCGATGCAATCCAGGCCGGGTGGATCGACGAAGCCGACTAAGCGTCACTTGGAGGTT‑AGGCAATCCCCCGGAACCCGCGTATTATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAAc                                                                                             <  2:324710/148‑1 (MQ=255)                                                         tCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACtt                                                                                       >  2:327032/1‑148 (MQ=255)                                                           gAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTTGAGGCATTCCCCCGGAACCCGCGTTAAATAGCCGGGTTTAATTTTTGGTAACAATCTTATTATGTAGTTTACCATGGATGTTAAAGGTCATTtc                                                                                     >  1:267748/1‑149 (MQ=255)                                                           gAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATgg                                                                                                      <  5:109944/131‑1 (MQ=255)                                                           gAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATgg                                                                                                      >  6:109944/1‑131 (MQ=255)                                                                aaTCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCcat                                                                                >  4:65540/1‑148 (MQ=255)                                                                aaTCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTCAATTTTTCGTAACACTCATAtt                                                                                                                      >  7:280361/1‑110 (MQ=255)                                                                aaTCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTCAATTTTTCGTAACACTCATAtt                                                                                                                      <  8:280361/110‑1 (MQ=255)                                                                    cAGGCCGCCTGGATCGACGAAGCCGAATAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCatcatc                                                                            >  2:211447/1‑148 (MQ=255)                                                                     aGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCatcatc                                                                            <  2:73546/147‑1 (MQ=255)                                                                     aGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCatcatc                                                                            >  7:208253/1‑147 (MQ=255)                                                                     aGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCaa                                                                          >  1:73546/1‑149 (MQ=255)                                                                       gccgccTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGc                                                                        >  8:277681/1‑149 (MQ=255)                                                                                   gacgaAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGtt                                                             <  1:164824/148‑1 (MQ=255)                                                                                   gacgaAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGtt                                                             >  2:164824/1‑148 (MQ=255)                                                                                    acgaAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCTGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTTTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGTTGTTGc                                                           >  5:243673/1‑149 (MQ=255)                                                                                    acgaAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGc                                                           >  3:199259/1‑149 (MQ=255)                                                                                    acgaAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGc                                                           >  4:324408/1‑149 (MQ=255)                                                                                    acgaAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGc                                                           <  4:199259/149‑1 (MQ=255)                                                                                             aCTAAGCGTCACTTGCAGGTT‑ATGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACg                                                   >  7:213874/1‑148 (MQ=255)                                                                                                 aGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACTCTGTTGCCAAAAACGTCAt                                               <  1:211447/148‑1 (MQ=255)                                                                                                 aGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCAt                                               <  3:65540/148‑1 (MQ=255)                                                                                                   cGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCgg                                            >  7:81567/1‑149 (MQ=255)                                                                                                    gTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGCTGCCAAAAACGTCATCgg                                            >  1:285879/1‑148 (MQ=255)                                                                                                         ttGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGa                                       >  6:249067/1‑148 (MQ=255)                                                                                                           gCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCgg                                            <  5:10468/141‑1 (MQ=255)                                                                                                           gCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCgg                                            >  6:10468/1‑141 (MQ=255)                                                                                                             aGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATcac                                   >  1:18149/1‑148 (MQ=255)                                                                                                                   aGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTc                              <  2:267748/148‑1 (MQ=255)                                                                                                                     gCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTtct                                                                                    <  6:284318/92‑1 (MQ=255)                                                                                                                     gCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTtct                                                                                    >  5:284318/1‑92 (MQ=255)                                                                                                                     gCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCt                           >  6:354277/1‑149 (MQ=255)                                                                                                                     gCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCt                           >  5:236979/1‑149 (MQ=255)                                                                                                                       aaTCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTc                         <  5:249067/149‑1 (MQ=255)                                                                                                                         tCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCg                        >  1:140761/1‑148 (MQ=255)                                                                                                                            cccGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATcac                                   <  7:194938/134‑1 (MQ=255)                                                                                                                            cccGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATcac                                   >  8:194938/1‑134 (MQ=255)                                                                                                                             ccGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCgaag                     >  2:82359/1‑147 (MQ=255)                                                                                                                                aaaCCCGCGAATTATACGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGcc                >  8:150194/2‑149 (MQ=255)                                                                                                                                   cccGCGAATTATACGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATAACAACTCGCTTCGAAGAAGCCGGc             >  7:98756/1‑149 (MQ=255)                                                                                                                                           taATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGCCGGCCTGAAAAt     >  4:158405/1‑149 (MQ=255)                                                                                                                                              ttCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGCCGGCCTGAAAATCGt  <  7:246169/149‑1 (MQ=255)                                                                                                                                              ttCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGCCGGCCTGAAAATCGt  >  1:143239/1‑149 (MQ=255)                                                                                                                                              ttCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGCCGGCCTGAAAATCGt  >  8:246169/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                    GGTGCTGGCATTGCCAGAGTTCAGCGACGATCCGTCACGCGGCGGTGAGAAAGTGCTCGAGGCAATCCAGGCCGCCTGGATCGACGAAGCCGACTAAGCGTCACTTGCAGGTT‑AGGCAATCCCCCGGAACCCGCGTATAATTCGCGGGTTTAATTTTTCGTAACACTCATATTCTGGAGTTTTCCATGGCTGTTCAACGTACTTTCTCCATCATCAAGCCTGACGCTGTTGCCAAAAACGTCATCGGCAAGATCACCACTCGCTTCGAAGAAGCCGGCCTGAAAATCGT  >  NC_002947/983446‑983734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑