| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 985,932 | G→C | 10.4% | E232Q (GAA→CAA) | pilF → | type IV pili biogenesis protein PilF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 985,932 | 0 | G | C | 10.4% | 142.3 / 7.3 | 48 | E232Q (GAA→CAA) | pilF | type IV pili biogenesis protein PilF |
| Reads supporting (aligned to +/- strand): ref base G (19/24); new base C (2/3); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01 | |||||||||||
AGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGAAA > NC_002947/985792‑986080 | aGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTggg < 4:162456/148‑1 (MQ=255)aGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTgg < 7:181901/147‑1 (MQ=255) gTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCACGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGc < 2:101149/148‑1 (MQ=255) ttGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCa < 3:167455/149‑1 (MQ=255) ttGTCCTACGAAAACAGGCATTATGTGCCGGCCCAGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCCAATTCGGCCa < 1:225721/149‑1 (MQ=255) cTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCaa < 3:42680/148‑1 (MQ=255) cTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCCAATTCGGCCAgtgt < 8:87605/148‑4 (MQ=255) aCGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAAtt < 6:105140/136‑1 (MQ=255) aCGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAAtt > 5:105140/1‑136 (MQ=255) cGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAAtt < 3:259899/147‑1 (MQ=255) cGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAAtt < 1:293393/147‑1 (MQ=255) aCAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGa < 6:186874/149‑1 (MQ=255) aGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACt > 8:268000/1‑149 (MQ=255) cATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAAt > 1:24301/1‑136 (MQ=255) cATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAAt < 2:24301/136‑1 (MQ=255) ttATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATc > 8:42887/1‑149 (MQ=255) ttATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAAATGGGCCAGCAATTACAACGACTTTATc < 4:330483/149‑1 (MQ=255) ggACTACTACGATCGTTTCAGCCAGTTGAGTGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGaa > 2:49280/1‑148 (MQ=255) ctactaCGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATc < 7:42887/149‑1 (MQ=255) ctacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAg > 3:231633/1‑148 (MQ=255) ctacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAg < 2:67497/148‑1 (MQ=255) ctacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAg < 7:268000/148‑1 (MQ=255) ctacCATCGTTTCAGCCAGTGGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGATTTGGGCCAGCAATTACATCGACTTTATCCCGGTACGCCGGAATATc < 8:283944/146‑1 (MQ=255) tacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTCCTGATGGGCAGCCGCCTTGCCAGGCTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAAAATCAGc > 5:22355/1‑148 (MQ=255) acGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCaa < 7:122075/149‑1 (MQ=255) gAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGa < 5:106784/141‑1 (MQ=255) gAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGa > 6:106784/1‑141 (MQ=255) gACCACGACGCCCGTAGCCTGCTTCTGGGCAGCCTCCTTTCCTGGGTGTTCGACGAGCATGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCTGTACGCCGGAATATGAGCAATACCTGTCGGAGCAACGATGAAc < 3:190447/149‑1 (MQ=255) gACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAAc > 4:153360/1‑149 (MQ=255) aCCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTTCAACGACTTTATCCCGGTACGCCGGAGTATCAGCCATACCTGTCGGAGCAACGATGAAcg > 8:87841/1‑149 (MQ=255) ccACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAAcgc > 3:254678/1‑149 (MQ=255) cACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAAcgcc > 5:220453/1‑149 (MQ=255) cgacgCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAAcgccgc < 4:254678/149‑1 (MQ=255) cccGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGTATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATc < 7:226290/149‑1 (MQ=255) ccGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATcc > 5:153917/1‑149 (MQ=255) gCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCTGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGGAACGATGAACGCCGCGCATCCCGaa < 6:22355/148‑1 (MQ=255) tgctgctgGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtag > 6:8763/1‑149 (MQ=255) ctgGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtagcag < 7:62374/147‑1 (MQ=255) aGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAAcccc > 4:324536/1‑149 (MQ=255) gtgtTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGt < 3:153360/149‑1 (MQ=255) gacgaGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCcgcg > 1:105338/1‑104 (MQ=255) gacgaGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCcgcg < 2:105338/104‑1 (MQ=255) cAGGGCACACTGGCCGAATTGGGCCAGCAATGACAACGACTTTATCCCGGTACGCCGGAATATCAGCACTACCTGTCGGCGCAACGCTGAACGCCGCGCATCCCGAAGTCGCAGTAGCGCCTGGCCCGCACCCCGGTGAGCGTTTgcgc > 3:172497/1‑149 (MQ=255) tgcTGGCCCAATTCGGCCAGCAATTACAACGACTTTATCACGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGccc > 4:129958/3‑149 (MQ=255) cTGGCCCAATTCGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc > 3:61125/1‑117 (MQ=255) cTGGCCCAATTCGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc < 4:61125/117‑1 (MQ=255) gCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGt > 8:184192/1‑146 (MQ=255) ccGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTgag > 5:316850/1‑148 (MQ=255) gAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGaaa > 7:273899/1‑149 (MQ=255) | AGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGTGTTCGACGAGCAGGGCACACTGGCCGAATTGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGAAA > NC_002947/985792‑986080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |