Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	989,167	A→C	10.0%	E340A (GAA→GCA)	hisS →	histidine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	989,167	0	A	C	10.0%	171.4 / 11.0	60	E340A (GAA→GCA)	hisS	histidine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (26/28);  new base C (3/3);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CTGGTCGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATCCT  >  NC_002947/989022‑989314                                                                                                                                                   |                                                                                                                                                    cTGGTCGAACAGATGGGTGGCAAGCCGACCAGCTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGaa                                                                                                                                                    >  1:35876/1‑148 (MQ=255)   ggTCGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAgg                                                                                                                                                >  4:279027/1‑149 (MQ=255)    ggcGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTCGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGc                                                                                                                                               <  3:282659/147‑1 (MQ=255)    gTCGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                                 <  7:235404/147‑1 (MQ=255)    gTCGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGc                                                                                                                                               >  7:30600/1‑149 (MQ=255)     tCGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                              <  2:738/149‑1 (MQ=255)           aGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTg                                                                                                                                        >  3:126040/1‑149 (MQ=255)                 gTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTggccggc                                                                                                                                  >  7:201104/1‑149 (MQ=255)                  tGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTggccggcc                                                                                                                                 >  3:192871/1‑149 (MQ=255)                    gCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTg                                                                                                                               >  1:265955/1‑149 (MQ=255)                      aaGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTgcg                                                                                                                             >  2:122050/1‑149 (MQ=255)                         ccGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCt                                                                                                                          <  4:126040/149‑1 (MQ=255)                                      gTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCtt                                                                                                              <  3:279027/149‑1 (MQ=255)                                         ggTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTgc                                                                                                            <  2:59783/148‑1 (MQ=255)                                               gCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGAcc                                                                                                      <  8:30600/148‑1 (MQ=255)                                                 cATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCAGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCt                                                                                                   >  2:201987/1‑149 (MQ=255)                                                     ggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGcc                                                                                               >  1:264638/1‑149 (MQ=255)                                                       cATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCgg                                                                                             <  3:109396/149‑1 (MQ=255)                                                             gcgcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTgc                                                                                       <  1:132986/149‑1 (MQ=255)                                                                 cTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                                   >  7:301970/1‑149 (MQ=255)                                                                 cTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                                   >  8:115822/1‑149 (MQ=255)                                                                 cTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                                   >  4:271123/1‑149 (MQ=255)                                                                    aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                                 <  2:265955/148‑1 (MQ=255)                                                                     tCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCa                                                                               <  7:115822/149‑1 (MQ=255)                                                                      ccTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                                           <  7:267759/136‑1 (MQ=255)                                                                      ccTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                                           >  8:267759/1‑136 (MQ=255)                                                                          ctgTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCaa                                                                          <  4:192871/149‑1 (MQ=255)                                                                             ttGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGTTCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGc                                                                       <  2:35876/149‑1 (MQ=255)                                                                             ttGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGc                                                                       <  2:185933/149‑1 (MQ=255)                                                                                 aaaCCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCggt                                                                   >  6:115922/1‑149 (MQ=255)                                                                                 aaaCCCTGGGCAAGGTGCCAGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGTCGGGCCTGCGCCTGGCAGTCAACGCCggt                                                                   <  1:201987/149‑1 (MQ=255)                                                                                    cccTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCggtggt                                                                <  8:154131/149‑1 (MQ=255)                                                                                     ccTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCGTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGACGttgttg                                                               >  8:233706/1‑149 (MQ=255)                                                                                         ggCAAGGAGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGc                                                             <  7:233706/147‑1 (MQ=255)                                                                                           cAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCt                                                         <  1:122050/149‑1 (MQ=255)                                                                                           cAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCt                                                         >  8:197058/1‑149 (MQ=255)                                                                                           cAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCt                                                         >  4:313695/1‑149 (MQ=255)                                                                                             aGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTc                                                       <  2:264638/149‑1 (MQ=255)                                                                                               gTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCGTc                                                       >  3:28531/1‑147 (MQ=255)                                                                                                tGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCaa                                                     >  8:314511/1‑148 (MQ=255)                                                                                                 gCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCTAAGGCTTGCGCGACCGCTTGCCGTGCCTGCGCCTGGCAGTCAACGCCTGTGGTGGCAGCTTCAaga                                                   <  8:201104/149‑1 (MQ=255)                                                                                                  ccGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAagag                                                  <  7:314511/149‑1 (MQ=255)                                                                                                  ccGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAaga                                                   <  3:271123/148‑1 (MQ=255)                                                                                                      aGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTc                                                                            <  3:318028/119‑1 (MQ=255)                                                                                                      aGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTc                                                                            >  4:318028/1‑119 (MQ=255)                                                                                                        tCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGtt                                            >  1:3346/1‑149 (MQ=255)                                                                                                           aTCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCaa                                         <  2:280526/149‑1 (MQ=255)                                                                                                            tCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTc                                           >  4:225673/1‑146 (MQ=255)                                                                                                               gccgccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGaaa                                     <  8:301970/149‑1 (MQ=255)                                                                                                                ccgccAGATCGCCGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAg                                    >  8:291798/1‑149 (MQ=255)                                                                                                                          gACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAaga                           <  6:102250/148‑1 (MQ=255)                                                                                                                              tccgccTGTTCGCCAAAGGCGCACAGGTGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGtggtgg                                                              <  3:67738/105‑1 (MQ=38)                                                                                                                              tccgccTGTTCGCCAAAGGCGCACAGGTGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGtggtgg                                                              >  4:67738/5‑109 (MQ=38)                                                                                                                                cgccTGTTCGCCAAAGGCGCACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTg                     >  4:126791/3‑148 (MQ=255)                                                                                                                                 aCCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCa                   >  6:299806/1‑149 (MQ=255)                                                                                                                                 aCCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCa                   >  1:283299/1‑149 (MQ=255)                                                                                                                                  ccTGTTCGCCAAAGGCGCACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCAc                  >  7:108611/1‑149 (MQ=255)                                                                                                                                  ccTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCa                   <  7:197058/148‑1 (MQ=255)                                                                                                                                         gcCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGc           <  2:283299/149‑1 (MQ=255)                                                                                                                                              aggCGCACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGa      <  3:126791/148‑1 (MQ=255)                                                                                                                                                  gAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATCCt  >  6:304393/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CTGGTCGAACAGATGGGTGGCAAGCCGACCA‑CTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATCCT  >  NC_002947/989022‑989314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑