Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	132,251	C→T	5.6%	A148V (GCC→GTC)	cc →	cytochrome c4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	132,251	0	C	T	5.6%	216.9 / 2.4	73	A148V (GCC→GTC)	cc	cytochrome c4
Reads supporting (aligned to +/- strand):  ref base C (32/35);  new base T (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CTCCAGCCAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTG  >  NC_002947/132108‑132399                                                                                                                                                |                                                                                                                                                     ctcCAGCCAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGc                                                                                                                                                  <  8:278660/148‑1 (MQ=255)    cAGCCAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGTCGGCTTcc                                                                                                                                              >  4:238667/1‑149 (MQ=255)    cAGCCAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTcc                                                                                                                                              >  5:177386/1‑149 (MQ=255)    cAGCCAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCGGGTCGGCTTcc                                                                                                                                              >  4:234160/1‑149 (MQ=255)       ccAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGc                                                                                                                                           >  1:309670/1‑149 (MQ=255)        cAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCa                                                                                                                                          >  6:292658/1‑149 (MQ=255)        cAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCa                                                                                                                                          >  1:285996/1‑149 (MQ=255)            aaGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTg                                                                                                                                      >  3:107142/1‑149 (MQ=255)             aGGCAGCGTGGGGGCGGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTgg                                                                                                                                     <  3:88724/149‑1 (MQ=255)              ggCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTggg                                                                                                                                    <  2:181681/149‑1 (MQ=255)                     tGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCaa                                                                                                                             <  5:292658/149‑1 (MQ=255)                       ggggCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAaca                                                                                                                           >  8:171114/1‑149 (MQ=255)                          gccgccGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGTCGGCTTCCCGCACCTGGGCGGGCAACACTc                                                                                                                        <  3:238667/149‑1 (MQ=255)                          gccgccGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGTCGGCTTCCCGCACCTGGGCGGGCAACACTc                                                                                                                        <  3:234160/149‑1 (MQ=255)                          gccgccGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTc                                                                                                                        <  3:306898/149‑1 (MQ=255)                          gccgccGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTc                                                                                                                        <  3:312015/149‑1 (MQ=255)                           ccgccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       <  2:309670/149‑1 (MQ=255)                            cgccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCTTCGCCCTGGCCgttgg                                                                                                                                                <  6:343878/123‑5 (MQ=255)                            cgccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCTTCGCCCTGGCCgttgg                                                                                                                                                >  5:343878/1‑119 (MQ=255)                            cgccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCgttgg                                                                                                                                                >  5:249414/1‑119 (MQ=255)                            cgccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCgttgg                                                                                                                                                <  6:249414/123‑5 (MQ=255)                            cgccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGc                                                                                                                      <  2:285996/149‑1 (MQ=255)                             gccgATCCCCAGCTGGTCGAGCGCGGCCGCGCGTTGTTCACTGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCa                                                                                                                     <  8:147084/149‑1 (MQ=255)                              ccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTgggcgggc                                                                                                                               <  3:151514/138‑1 (MQ=255)                              ccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTgggcgggc                                                                                                                               >  4:151514/1‑138 (MQ=255)                              ccgATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAg                                                                                                                    <  4:107142/149‑1 (MQ=255)                                   cccAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGt                                                                                                               >  1:192146/1‑149 (MQ=255)                                    ccAAGCTGGTCTAGCGCGGCCGCGCGTTGTTCAATTGCGCCGACCTGGAAAACGTCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGTCATCGCCCTGGGCGTCTTCGAGCACCTGGGCGGGCAACACTCGGAGTACGTg                                                                                                              <  2:18730/149‑1 (MQ=255)                                    ccAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCGGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       <  1:27831/140‑1 (MQ=255)                                    ccAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       >  2:27831/1‑140 (MQ=255)                                    ccAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTg                                                                                                              <  1:333624/149‑1 (MQ=255)                                    ccAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTg                                                                                                              <  7:171114/149‑1 (MQ=255)                                     cAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       >  1:27152/1‑139 (MQ=255)                                     cAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       <  2:27152/139‑1 (MQ=255)                                             tCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAagcag                                                                                                     <  5:6328/149‑1 (MQ=255)                                             tCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAagcag                                                                                                     <  6:177386/149‑1 (MQ=255)                                                   gcgGCCGCGCGTTGTTCAATGGCGGCTACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGACATCGCCCTGGCCGGCTTCCCGCACCTgg                                                                                                                                     <  8:169758/111‑1 (MQ=255)                                                   gcgGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGACATCGCCCTGGCCGGCTTCCCGCACCTgg                                                                                                                                     >  7:169758/1‑111 (MQ=255)                                                    cgGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       >  4:189240/1‑124 (MQ=255)                                                    cgGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCg                                                                                                                       <  3:189240/124‑1 (MQ=255)                                                            cgTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCcgc                                                                                      <  4:141962/149‑1 (MQ=255)                                                                 ttCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAag                                                                                                        <  7:277150/126‑1 (MQ=255)                                                                 ttCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAag                                                                                                        >  8:277150/1‑126 (MQ=255)                                                                   cAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTgg                                                                                                             <  8:59363/119‑1 (MQ=255)                                                                   cAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGc                                                                                >  6:235138/1‑148 (MQ=255)                                                                     aTGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAAc                                                                             >  3:205086/1‑149 (MQ=255)                                                                       ggcggcGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCg                                                                           >  8:172237/1‑149 (MQ=255)                                                                                         ggCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGc                                                         >  5:1475/1‑149 (MQ=255)                                                                                           cATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCa                                                       <  4:205086/149‑1 (MQ=255)                                                                                            aTGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCAt                                                      >  4:144061/1‑149 (MQ=255)                                                                                            aTGCCGGCCTGCACTCGCTGACACTAGCCAAACGACCCGGGCATCGCCCTGGACGGCATCCCGCACCTGGTCGGCCAACACTCGCAGTACGTGGCCAAGCAGCTCACTAACTTCCTCGAAGGCAACCCCACCAACGAAGCCGAAGCCaa                                                      >  4:89452/1‑148 (MQ=255)                                                                                                    cTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATgc                                              >  6:136294/1‑149 (MQ=255)                                                                                                    cTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATgc                                              <  5:235138/149‑1 (MQ=255)                                                                                                      gCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATgcg                                             >  4:330321/1‑148 (MQ=255)                                                                                                       cACTGGCTGCCACTCGCCCAACGGCGCGGTCATCGCCCTGGCCGGCATCCCGCACCTGGGCGGGCAACACTCGCAGTACCTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCa                                           <  3:278976/149‑1 (MQ=255)                                                                                                            gCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATc                                      <  7:160598/149‑1 (MQ=255)                                                                                                                  aCTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTgg                                 >  8:212497/1‑148 (MQ=255)                                                                                                                      gCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGc                            >  4:113421/1‑149 (MQ=255)                                                                                                                       cccAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCt                           <  3:144061/149‑1 (MQ=255)                                                                                                                        ccAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCt                           >  8:250626/1‑148 (MQ=255)                                                                                                                         cAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGa                         <  2:192146/149‑1 (MQ=255)                                                                                                                            cGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCACCCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAg                             >  7:4289/1‑142 (MQ=255)                                                                                                                            cGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAg                             <  8:4289/142‑1 (MQ=255)                                                                                                                              gcgcGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAAc                    >  4:233374/1‑149 (MQ=255)                                                                                                                                    gCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGAc              <  6:1475/149‑1 (MQ=255)                                                                                                                                      aTCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTAAGCAACCATGACAt            >  1:118333/1‑149 (MQ=255)                                                                                                                                       tCGCCCTGGCCGGCTTCCCGCACCAGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATc           >  2:17539/1‑149 (MQ=255)                                                                                                                                           ccTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAg       >  4:96179/1‑149 (MQ=255)                                                                                                                                            cTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGc      <  7:212497/149‑1 (MQ=255)                                                                                                                                             tGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGCTGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCg     >  6:326526/1‑149 (MQ=255)                                                                                                                                             tGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGaa        <  7:63659/146‑1 (MQ=255)                                                                                                                                             tGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGaa        >  8:63659/1‑146 (MQ=255)                                                                                                                                               gCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGtt   <  3:113421/149‑1 (MQ=255)                                                                                                                                                ccGGCTTCCCGCACCTGGGCGGGCAACACTTGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTg  <  7:172237/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CTCCAGCCAAAAAGGCAGCGTGGGGGCCGCCGATCCCAAGCTGGTCGAGCGCGGCCGCGCGTTGTTCAATGGCGGCGACCTGGAAAAGGGCATGCCGGCCTGCACTGGCTGCCACTCGCCCAACGGCGCGGGCATCGCCCTGGCCGGCTTCCCGCACCTGGGCGGGCAACACTCGCAGTACGTGGCCAAGCAGCTCACTGACTTCCGCGAAGGCAACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTG  >  NC_002947/132108‑132399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑