Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,013,255	A→T	14.0%	intergenic (+83/+48)	prfC → / ← potF‑I	peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,013,255	0	A	T	14.0%	101.0 / 7.6	43	intergenic (+83/+48)	prfC/potF‑I	peptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base T (3/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

CCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGT  >  NC_002947/1013109‑1013400                                                                                                                                                   |                                                                                                                                                  ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcc                                                                                                                                                 >  2:218515/1‑149 (MQ=255)  cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt                                                                                                                                                <  5:148692/149‑1 (MQ=255)     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                              <  1:46501/148‑1 (MQ=25)     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt                                                                                                                                              <  3:121781/148‑1 (MQ=255)       gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg                                                                                                                                            >  5:171236/1‑148 (MQ=255)       gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAACACAGGAGGGGATGTGATTCCTGgggg                                                                                                                                           >  2:293414/1‑149 (MQ=38)          gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGc                                                                                                                                        >  1:47091/1‑149 (MQ=255)             ggAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTACCACACGAATCACCAGACCTGGATGTAATGTGATACCTGTGGGAGCggg                                                                                                                                     >  1:19091/1‑149 (MQ=39)              gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGc                                                                                                                                    <  7:207304/149‑1 (MQ=255)                                   aaGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAg                                                                                                               <  3:118693/149‑1 (MQ=255)                                    aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCCCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAgg                                                                                                              >  6:347887/1‑149 (MQ=255)                                        ccGCGCTACCCGCGAGCACCATTACGCCCTGAAATCGGGGCTGCTTGGCAGCCCTTCGCGGGCACGCCCGCTCCCCCACTAATCCCCCGCCCTGTAGGTCATGTGAAACCTGTGGTAGCGGGCGTGCCCGCGAAGGGCCGCTAggcgg                                                                                                           <  2:19091/148‑1 (MQ=25)                                           cgcTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGcccc                                                                                                       <  6:171236/149‑1 (MQ=255)                                                        cACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCACCCACAGGAATAACCACACCAGGAGGTCAAGTTATACCTGTGGGAGCGGGCGTGCCCGCGCACGGCCCCAAGGCGGGCCCAAATAAATTTGCg                                                                                          >  6:187871/1‑149 (MQ=25)                                                             ttAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCttt                                                                                     <  2:47091/149‑1 (MQ=255)                                                                     tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCAACCACAGGAATAACCAGACCTGGACGTCATGTGAAACCTCTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAAGGCGGCCCCCTTTCAATTTCAGGCTTTGATAATgg                                                                             >  4:108737/1‑149 (MQ=25)                                                                        aagcGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCCTACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                                          <  5:187871/146‑1 (MQ=255)                                                                              ggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCttt                                                                                     <  3:121616/132‑1 (MQ=255)                                                                                ctgctTTGCAGCCCTTCGCGGCCACGCCCGCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCTGTGGGAGGGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                                  <  1:293414/149‑1 (MQ=255)                                                                                             cTTCGCGGGCACGCCCGCTCCCACAGCAATCACCAGACCTGGAGGTAATGTGATACCTGTGGGAGGGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGt                                                     <  2:14225/149‑1 (MQ=255)                                                                                                 gcgGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCAGTTCAATTTCCGGCGTTGATACTGGTCCAGAGTCGCGTACGAATACGGTCGAt                                                 >  8:224352/1‑149 (MQ=255)                                                                                                       aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGc                                           <  1:218515/149‑1 (MQ=255)                                                                                                           ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                                        >  4:299925/1‑148 (MQ=255)                                                                                                                 ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTgg                                                                             >  2:125247/1‑105 (MQ=14)                                                                                                                 ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTgg                                                                             <  1:125247/105‑1 (MQ=14)                                                                                                                 ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                                 >  4:191778/1‑149 (MQ=255)                                                                                                                 ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                                 >  5:261227/1‑149 (MQ=255)                                                                                                                 ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                                 >  1:308578/1‑149 (MQ=255)                                                                                                                     aGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa                             <  6:277191/149‑1 (MQ=255)                                                                                                                       gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc                           >  5:176733/1‑149 (MQ=255)                                                                                                                       gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc                           >  4:160263/1‑149 (MQ=255)                                                                                                                          tCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCa                        <  6:261227/149‑1 (MQ=255)                                                                                                                            acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa                                                                       <  4:288554/96‑1 (MQ=17)                                                                                                                            acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa                                                                       >  3:288554/5‑100 (MQ=17)                                                                                                                            aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                      <  3:299925/149‑1 (MQ=255)                                                                                                                              atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg                          <  5:89868/141‑1 (MQ=255)                                                                                                                              atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg                          >  6:89868/3‑143 (MQ=255)                                                                                                                                 aCCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATa                                                                                 <  7:185271/85‑1 (MQ=255)                                                                                                                                 aCCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATa                                                                                 >  8:185271/1‑85 (MQ=255)                                                                                                                                   cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                     >  7:61497/1‑143 (MQ=255)                                                                                                                                   cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                     <  8:61497/143‑1 (MQ=255)                                                                                                                                      gAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGccc            <  3:160263/149‑1 (MQ=255)                                                                                                                                                 aTACGTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCtcgt  <  5:211163/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGT  >  NC_002947/1013109‑1013400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑