Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,025,784 T→G 9.1% D395A (GAC→GCC)  dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,025,7840TG 9.1% 161.9 / 7.5 55D395A (GAC→GCC) dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (28/22);  new base G (3/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TAGAGGGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCT  >  NC_002947/1025637‑1025931
                                                                                                                                                   |                                                                                                                                                   
tAGAGGGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTc                                                                                                                                                    >  4:183547/1‑149 (MQ=255)
         cccAGCCAGTTGTCCGGGCCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCTTGCTCACCGTTTTTCATGCGCTTTAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGa                                                                                                                                            <  6:171646/148‑1 (MQ=255)
           cagccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTg                                                                                                                                              <  3:34791/144‑1 (MQ=255)
           cagccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTg                                                                                                                                              >  4:34791/1‑144 (MQ=255)
                agTTGTCCTGTTCGAAGTTGTAACCGTTCCAGCCGATCAGTGCGATGTCGTGCTCACCGTTTTTCATGCTCTTGATGTATTCGCCTCATTAGTAGCTGACGATGCTCAACTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCg                                                                                                                                    <  7:219893/149‑1 (MQ=255)
                agTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCg                                                                                                                                    <  7:65659/149‑1 (MQ=255)
                agTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCg                                                                                                                                    >  7:25833/1‑149 (MQ=255)
                           tCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCtt                                                                                                                         <  3:280623/149‑1 (MQ=255)
                           tCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCtt                                                                                                                         >  1:49536/1‑149 (MQ=255)
                           tCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCtt                                                                                                                         >  4:280623/1‑149 (MQ=255)
                           tCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCtt                                                                                                                         <  2:49536/149‑1 (MQ=255)
                             gCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGc                                                                                                                                  >  2:33718/1‑138 (MQ=255)
                             gCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGc                                                                                                                                  <  1:33718/138‑1 (MQ=255)
                             gCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTgg                                                                                                                       >  1:316856/1‑149 (MQ=255)
                             gCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTgg                                                                                                                       >  5:29085/1‑149 (MQ=255)
                              ccGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTgg                                                                                                                       >  3:196930/1‑148 (MQ=255)
                                     cACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTggg                                                                                                                <  5:11211/148‑1 (MQ=255)
                                      aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGt                                                                                                              >  8:232070/1‑149 (MQ=255)
                                      aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGt                                                                                                              >  4:113857/1‑149 (MQ=255)
                                        cGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTg                                                                                                            >  4:322350/1‑149 (MQ=255)
                                         ggTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGt                                                                                                           <  6:29085/149‑1 (MQ=255)
                                                ccGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaag                                                                                                                                       >  1:218317/1‑112 (MQ=255)
                                                ccGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaag                                                                                                                                       <  2:218317/114‑3 (MQ=255)
                                                  gATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaa                                                                                                                                        >  4:150822/1‑110 (MQ=255)
                                                  gATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaa                                                                                                                                        <  3:150822/111‑2 (MQ=255)
                                                                 gTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGcc                                                                                    <  8:13437/148‑1 (MQ=255)
                                                                       aCCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAggg                                                                             >  6:130501/1‑149 (MQ=255)
                                                                          gTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGa                                                                          >  1:95890/1‑149 (MQ=255)
                                                                                 aTGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGt                                                                   <  3:113857/149‑1 (MQ=255)
                                                                                 aTGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGt                                                                   <  5:130501/149‑1 (MQ=255)
                                                                                  tGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTg                                                                  >  2:94544/1‑149 (MQ=255)
                                                                                   gcgcTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGc                                                                 >  1:69890/1‑149 (MQ=255)
                                                                                     gcTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGc                                                                 >  2:248630/1‑147 (MQ=255)
                                                                                           ggTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGttt                                                          <  2:316856/148‑1 (MQ=255)
                                                                                            gTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGa                                                        <  1:248630/149‑1 (MQ=255)
                                                                                              aTTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCtt                                                                                                                         <  8:85682/82‑1 (MQ=255)
                                                                                              aTTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCtt                                                                                                                         >  7:85682/1‑82 (MQ=255)
                                                                                               ttCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGaca                                                      <  3:183547/148‑1 (MQ=255)
                                                                                                  gCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAACATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGAtc                                                                        <  6:167580/127‑1 (MQ=255)
                                                                                                  gCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAACATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGAtc                                                                        >  5:167580/1‑127 (MQ=255)
                                                                                                    cccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCgg                                                                    >  7:14669/1‑129 (MQ=255)
                                                                                                    cccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCgg                                                                    <  8:14669/129‑1 (MQ=255)
                                                                                                        ttCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCctgct                                            >  8:49419/1‑149 (MQ=255)
                                                                                                               cTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAg                                     <  7:232070/149‑1 (MQ=255)
                                                                                                               cTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGATTGGCATTGGGGTTGTACTGGCGTTGCACCGGCATCGCCCACAGGGTGATCTCGGTGCCACGTTTGACACCCGCCTCCTGGATCAg                                     >  7:44434/1‑149 (MQ=255)
                                                                                                                tGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGc                                    >  5:157504/1‑149 (MQ=255)
                                                                                                                     aTGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCTTTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCt                               <  8:25833/149‑1 (MQ=255)
                                                                                                                             cTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTg                              <  5:173302/142‑1 (MQ=255)
                                                                                                                             cTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTg                              >  6:173302/1‑142 (MQ=255)
                                                                                                                               tGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTcc                     >  5:217169/1‑149 (MQ=255)
                                                                                                                                    gccgaaatGCTCCAGGCGGACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGgcgc                >  5:350891/8‑149 (MQ=25)
                                                                                                                                            gCTCCCGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCAGTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACgg          <  4:196930/147‑1 (MQ=255)
                                                                                                                                              tCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGCTCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCg      >  2:112194/1‑149 (MQ=255)
                                                                                                                                              tCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACACGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCg      >  6:6347/1‑149 (MQ=255)
                                                                                                                                                  gTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCt  >  7:91755/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TAGAGGGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCT  >  NC_002947/1025637‑1025931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑