Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,030,800 G→C 10.8% E286D (GAG→GAC) ‡ PP_0887 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,030,8000GC10.8% 214.4 / 14.4 74E286D (GAG→GAC) ‡PP_0887sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base C (4/4);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

ACCCAGGCCGCCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACG  >  NC_002947/1030654‑1030948
                                                                                                                                                    |                                                                                                                                                    
aCCCAGGCCGCCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGAc                                                                                                                                                    >  6:169634/1‑149 (MQ=255)
  ccAGGCCGCCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACtt                                                                                                                                                  <  8:10584/149‑1 (MQ=255)
    aGGCCGCCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTg                                                                                                                                                >  4:235467/1‑149 (MQ=255)
    aGGCCGCCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTg                                                                                                                                                >  3:304081/1‑149 (MQ=255)
          ccgGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAg                                                                                                                                          <  7:5203/149‑1 (MQ=255)
           cgGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGc                                                                                                                                         <  4:201462/149‑1 (MQ=255)
             gTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGTCTCTTTGC‑ACAGCttt                                                                                                                                      <  3:188237/149‑3 (MQ=255)
              tGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTT‑GCAAAGTCTCTTTGC‑ACAGCttt                                                                                                                                      <  7:124118/149‑3 (MQ=255)
                 cgccCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTgc                                                                                                                                   <  4:131388/149‑1 (MQ=255)
                   cccATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTgcgc                                                                                                                                 >  8:85446/1‑149 (MQ=255)
                   cccATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTgcgc                                                                                                                                 <  3:310230/149‑1 (MQ=255)
                     cATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGTCTCTTTGCa                                                                                                                                              >  1:197181/1‑134 (MQ=255)
                     cATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGTCTCTTTGCa                                                                                                                                              <  2:197181/134‑1 (MQ=255)
                      aTGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCa                                                                                                                              <  4:67741/149‑1 (MQ=255)
                       tGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTT‑GCAAAGTCTCTTTGCaca                                                                                                                                            <  1:207552/135‑3 (MQ=255)
                       tGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTGTT‑GCAAAGTCTCTTTGCaca                                                                                                                                            >  2:207552/1‑133 (MQ=255)
                        gAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGc                                                                                                                            >  5:73981/1‑149 (MQ=255)
                           cTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCg                                                                                                                          >  1:241916/1‑148 (MQ=255)
                              ggTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGc                                                                                                                      >  2:248251/1‑149 (MQ=255)
                                tACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGcc                                                                                                                     >  2:247642/1‑148 (MQ=255)
                                   gccgcTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAAcc                                                                                                                 >  1:322359/1‑149 (MQ=255)
                                       cTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGgcg                                                                                                             <  4:221975/149‑1 (MQ=255)
                                          gCGACCATGAGCGTGCTGATAAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCt                                                                                                          >  1:306630/1‑149 (MQ=255)
                                             aCCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGc                                                                                                       <  6:73981/149‑1 (MQ=255)
                                               cATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCa                                                                                                     <  2:74982/149‑1 (MQ=255)
                                                    gCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTg                                                                                                <  1:248251/149‑1 (MQ=255)
                                                     cGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTgg                                                                                               <  3:235467/149‑1 (MQ=255)
                                                       tGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAg                                                                                             <  7:85446/149‑1 (MQ=255)
                                                          tGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACa                                                                                                                                           >  4:32323/1‑100 (MQ=255)
                                                          tGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACa                                                                                                                                           <  3:32323/100‑1 (MQ=255)
                                                             tCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAt                                                                                       >  7:16135/1‑149 (MQ=255)
                                                               aaCGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAtgt                                                                                     >  8:74540/1‑149 (MQ=255)
                                                               aaCGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAtgt                                                                                     >  3:110562/1‑149 (MQ=255)
                                                                aCGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAtgtg                                                                                    <  4:304081/149‑1 (MQ=255)
                                                                        cgcCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctg                                                                            <  4:110562/149‑1 (MQ=255)
                                                                         gcCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctgg                                                                           >  5:118774/1‑149 (MQ=255)
                                                                          ccAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCctggc                                                                          <  2:306630/149‑1 (MQ=255)
                                                                              gACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAt                                                                                       <  3:61274/132‑1 (MQ=255)
                                                                              gACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGAt                                                                                       >  4:61274/1‑132 (MQ=255)
                                                                               aCCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGAc                                                                     >  6:28179/1‑149 (MQ=255)
                                                                                 cacaCCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGAc                                                                     >  6:297167/1‑147 (MQ=255)
                                                                                 cacaCCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGa                                                                   <  2:241916/149‑1 (MQ=255)
                                                                                   cacCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAgg                                                                 <  2:664/149‑1 (MQ=255)
                                                                                    acCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑TGGCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAgg                                                                 >  1:267500/1‑149 (MQ=255)
                                                                                    acCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCACGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGgc                                                                >  7:287672/1‑149 (MQ=255)
                                                                                           cGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAc                                                         >  2:18837/1‑149 (MQ=255)
                                                                                           cGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTCGCCTTCGTGGAGCAGGATCTGACCGCCTGGCTGGACGAGGCGCTCAAc                                                         >  8:289073/1‑149 (MQ=255)
                                                                                            gCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAcc                                                        >  8:192059/1‑149 (MQ=255)
                                                                                            gCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAAcc                                                        <  1:247642/149‑1 (MQ=255)
                                                                                                  gCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTgg                                                   >  1:222274/1‑148 (MQ=255)
                                                                                                    aggaggACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCAc                                                >  3:211566/1‑149 (MQ=255)
                                                                                                     ggaggaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCAcc                                               >  5:236741/1‑149 (MQ=255)
                                                                                                       aggaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTg                                             <  7:192059/149‑1 (MQ=255)
                                                                                                         gaCCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGAt                                           <  5:169634/149‑1 (MQ=255)
                                                                                                          aCCTGCAAATACTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGAt                                           <  8:16135/148‑1 (MQ=255)
                                                                                                            cTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATgcg                                        <  4:211566/149‑1 (MQ=255)
                                                                                                              gCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAAc                                                                                                                  >  3:265299/1‑73 (MQ=255)
                                                                                                              gCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAAc                                                                                                                  <  4:265299/73‑1 (MQ=255)
                                                                                                               cAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGccc                                     <  1:7902/149‑1 (MQ=255)
                                                                                                                aaaTCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCg                                    >  2:82612/1‑149 (MQ=255)
                                                                                                                   tcctccAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCgg                                   >  6:269320/1‑147 (MQ=255)
                                                                                                                   tcctccAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGaa                                 <  7:74540/149‑1 (MQ=255)
                                                                                                                      tccAGTACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGc                               >  2:267107/1‑148 (MQ=255)
                                                                                                                      tccAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGc                               >  6:328428/1‑148 (MQ=255)
                                                                                                                        cAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCa                             <  5:328428/148‑1 (MQ=255)
                                                                                                                           gACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTa                         <  2:222274/149‑1 (MQ=255)
                                                                                                                           gACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTa                         >  2:268809/1‑149 (MQ=255)
                                                                                                                           gACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTCATGCGCCCGGAAGCCAGcaa                         >  2:276786/1‑147 (MQ=255)
                                                                                                                                  tAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTc                  <  5:269320/149‑1 (MQ=255)
                                                                                                                                     aGCTGTT‑GCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAg               >  1:251342/1‑149 (MQ=255)
                                                                                                                                      gCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCACAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgc              >  8:300111/1‑149 (MQ=255)
                                                                                                                                       cTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgcg             >  6:332532/1‑149 (MQ=255)
                                                                                                                                        tgtt‑gCAAAGTCTCTTTGC‑ACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAgcgc            >  4:273445/4‑149 (MQ=255)
                                                                                                                                              cAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTgc        <  4:59715/149‑1 (MQ=255)
                                                                                                                                                  gagaCTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGa    >  6:78044/1‑149 (MQ=255)
                                                                                                                                                    gaCTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACg  <  2:267500/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCCAGGCCGCCGGTGCCGCCCATGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGATCAACGAAATGCGCCAGGACCACACCGACCCGCTGTTGCAGGAGGACCTGCAAATCCTCCAGGACCAGGTAAAGCTG‑T‑GCAAAGAGACTTTGCAACAGCTGGTGCGCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACG  >  NC_002947/1030654‑1030948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑