Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,031,246	G→C	5.2%	G435A (GGC→GCC)	PP_0887 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,031,246	0	G	C	5.2%	293.7 / 3.3	90	G435A (GGC→GCC)	PP_0887	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (43/42);  new base C (3/2);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTGTACCGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCC  >  NC_002947/1031099‑1031394                                                                                                                                                    |                                                                                                                                                      cTGGTGTACCGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGc                                                                                                                                                      <  6:231218/149‑1 (MQ=255)    gtgtACCGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCAcc                                                                                                                                                   >  1:40786/1‑149 (MQ=255)        aCCGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGa                                                                                                                                               >  5:264545/1‑149 (MQ=255)         ccGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGAc                                                                                                                                              >  2:314464/1‑149 (MQ=255)          cGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGAcc                                                                                                                                             <  1:314464/149‑1 (MQ=255)          cGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGAc                                                                                                                                              >  4:140997/1‑148 (MQ=255)          cGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGAc                                                                                                                                              <  3:140997/148‑1 (MQ=255)            ccagccaTTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGAGCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGCCACCCTgttcat                                                                                                                                           <  1:337194/149‑6 (MQ=255)              agccaTTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAAc                                                                                                                                         <  2:310854/149‑1 (MQ=255)                 caTTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTgc                                                                                                                                      <  2:144960/149‑1 (MQ=255)                      ccGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTg                                                                                                                                 >  6:168956/1‑149 (MQ=255)                        gAAGCCATTGGCAACCCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCCTGCGGGCGTCTCGGTGCAACTCTATAGTCATGAACACGGTGGCACCCTGACCCAACTGCGCCTGcc                                                                                                                               >  7:278905/1‑149 (MQ=255)                        gAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTCTAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGcc                                                                                                                               >  6:20291/1‑149 (MQ=255)                        gAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGcc                                                                                                                               >  5:137376/1‑149 (MQ=255)                        gAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGcc                                                                                                                               >  8:322209/1‑149 (MQ=255)                         aaGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGccc                                                                                                                              >  3:230666/1‑149 (MQ=255)                          aGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCt                                                                                                                             <  7:20107/149‑1 (MQ=255)                             cATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATg                                                                                                                          <  2:78459/149‑1 (MQ=255)                              aTTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATgg                                                                                                                         >  1:292490/1‑149 (MQ=255)                                  gCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAg                                                                                                                     >  3:319609/1‑149 (MQ=255)                                   cAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGc                                                                                                                    <  5:168956/149‑1 (MQ=255)                                    aaaCCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGc                                                                                                                    >  5:183301/1‑148 (MQ=255)                                       ccTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCgagg                                                                                                                <  4:230666/149‑1 (MQ=255)                                        cTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCgagga                                                                                                               <  6:137376/149‑1 (MQ=255)                                         ttttATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGTCCAGCGTGACCCGTGCGGGCGGCTCGCTGAAACTCTATAGTCATGAACAGGGTGCCACCCTCACCGAACTGCGCCTGCCCTATGGCAAGCgag                                                                                                                 >  7:115063/1‑146 (MQ=255)                                         ttttATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCgag                                                                                                                 >  2:189735/1‑146 (MQ=255)                                            tattaCCACCATTGTCAAAGGCTTGGGCGTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑t                                                                                                           <  8:278905/148‑1 (MQ=255)                                                accaccAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑tgaat                                                                                                       >  6:134640/1‑148 (MQ=255)                                                accaccAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑tgaat                                                                                                       <  1:309920/148‑1 (MQ=255)                                                 ccaccaAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑tgaatga                                                                                                     >  4:286707/1‑149 (MQ=255)                                                 ccaccaAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑tgaatga                                                                                                     <  1:189735/149‑1 (MQ=255)                                                  caccaAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAatgat                                                                                                    >  1:335388/1‑149 (MQ=255)                                                  caccaAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAatgat                                                                                                    <  3:286707/149‑1 (MQ=255)                                                    ccaAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGTACTGCGCCTGCCCTATGGCAAGCga                                                                                                                  <  5:67508/134‑1 (MQ=255)                                                    ccaAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGGACTGCGCCTGCCCTATGGCAAGCga                                                                                                                  >  6:67508/1‑134 (MQ=255)                                                     caAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑t                                                                                                           >  3:75143/1‑139 (MQ=255)                                                     caAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGa‑t                                                                                                           <  4:75143/139‑1 (MQ=255)                                                     caAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAg                                                                                                 >  7:39591/1‑149 (MQ=255)                                                      aaGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCgagga                                                                                                               <  3:243937/135‑1 (MQ=255)                                                      aaGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCgagga                                                                                                               >  4:243937/1‑135 (MQ=255)                                                            aaaGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCgaaga                                                                                           >  2:234531/1‑148 (MQ=255)                                                            aaaGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCgaaga                                                                                           >  2:298308/1‑148 (MQ=255)                                                                gCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAAcc                                                                                      <  6:183301/149‑1 (MQ=255)                                                                gCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAAc                                                                                       >  3:329856/1‑148 (MQ=255)                                                                       cctgggcctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCg                                                                                >  5:231165/1‑148 (MQ=255)                                                                        ctgggcctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGaa                                                                              <  7:322209/149‑1 (MQ=255)                                                                         tgggcctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGaaa                                                                             <  4:2577/149‑1 (MQ=255)                                                                           ggcctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGaaa                                                                             <  2:292490/147‑1 (MQ=255)                                                                           ggcctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGc                                                                           <  2:40786/149‑1 (MQ=255)                                                                             cctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCg                                                                          >  6:321722/1‑148 (MQ=255)                                                                              ctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTCTAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGaa                                                                        <  5:20291/149‑1 (MQ=255)                                                                              ctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCg                                                                          >  6:90834/1‑147 (MQ=255)                                                                              ctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGaa                                                                        <  1:298308/149‑1 (MQ=255)                                                                              ctgTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGaa                                                                        <  4:329856/149‑1 (MQ=255)                                                                                gTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGaa                                                                        >  5:170829/1‑147 (MQ=255)                                                                                 ttCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGTGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAaga                                                                      <  4:319609/148‑1 (MQ=255)                                                                                  tCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGc                                                                    >  8:297773/1‑149 (MQ=255)                                                                                    ttGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAg                                                                            >  1:180031/1‑139 (MQ=255)                                                                                    ttGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAg                                                                            <  2:180031/139‑1 (MQ=255)                                                                                    ttGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTg                                                                  >  3:40925/1‑149 (MQ=255)                                                                                      gAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCt                                                                   <  1:234531/146‑1 (MQ=255)                                                                                       aGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGcc                                                                <  6:264545/148‑1 (MQ=255)                                                                                        gCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCg                                                               >  5:251017/1‑148 (MQ=255)                                                                                          aaGGCCAGCGTGTCGCGTGCGGGCGGCTCTTTGAAACTCTATAGTCATGAACCTGGTGGCACCCTGAGCGAACTGCGCCTGCCCTTTGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTAGAAAGCGAAGAGCTGCCTCAc                                                            <  8:115063/149‑1 (MQ=255)                                                                                            ggCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGc                                                                                                >  3:71391/1‑111 (MQ=255)                                                                                            ggCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGc                                                                                                <  4:71391/111‑1 (MQ=255)                                                                                             gCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACctg                                                         <  4:40925/149‑1 (MQ=255)                                                                                               cAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACctgct                                                       >  7:298405/1‑149 (MQ=255)                                                                                                    tGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCtgg                                                  <  2:335388/149‑1 (MQ=255)                                                                                                      aCCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCtggt                                                 <  8:298405/148‑1 (MQ=255)                                                                                                               ggcggcTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATTTCATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGa                                        <  1:276052/149‑1 (MQ=255)                                                                                                               ggcggcTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGAt                                       <  5:134640/149‑1 (MQ=255)                                                                                                                  ggcTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATg                                      <  8:308076/147‑1 (MQ=255)                                                                                                                  ggcTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATg                                      >  7:308076/1‑147 (MQ=255)                                                                                                                   gcTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCa                                   >  1:211266/1‑149 (MQ=255)                                                                                                                       ggTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCAcc                                 <  1:54233/147‑1 (MQ=255)                                                                                                                       ggTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCt                                <  7:297773/148‑1 (MQ=255)                                                                                                                        gTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCtt                               >  6:205210/1‑148 (MQ=255)                                                                                                                             aCTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCg                         >  8:271788/1‑149 (MQ=255)                                                                                                                                 tataGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGt                      >  4:154328/1‑148 (MQ=255)                                                                                                                                   taGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTc                     <  3:154328/147‑1 (MQ=255)                                                                                                                                   taGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCa                    <  6:170829/148‑1 (MQ=255)                                                                                                                                   taGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTAGATGACGATGCCACCTTCACCCGGGTCAt                   >  7:300224/1‑149 (MQ=255)                                                                                                                                      tCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGc                >  4:59591/1‑149 (MQ=255)                                                                                                                                      gttcggtcAGGGTGCCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGc                >  8:315795/8‑149 (MQ=255)                                                                                                                                       cATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGcc               >  7:292676/1‑149 (MQ=255)                                                                                                                                       cATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGcc               >  7:297268/1‑149 (MQ=255)                                                                                                                                           gtcAGGGTGCCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGATGATGCCACCTTCACCCg                         >  1:234045/3‑135 (MQ=255)                                                                                                                                           gtcAGGGTGCCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGATGATGCCACCTTCACCCg                         <  2:234045/133‑1 (MQ=255)                                                                                                                                               gggTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCAt       <  6:251017/149‑1 (MQ=255)                                                                                                                                                    gCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGAccc  <  2:211266/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CTGGTGTACCGCCAGCCATTGCCGAAGCCATTGGCAAACCTTTTATTACCACCAAGGGCAAAGGCTTCGGCCTGGGCCTGTTCTTGAGCAAGGCCAGCGTGACCCGTGCGGGCGGCTCGGTGAAACTCTATAGTCATGAACAGGGTGGCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGA‑TGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCC  >  NC_002947/1031099‑1031394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑