Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,033,803 T→A 6.5% D141E (GAT→GAA)  PP_0891 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,033,8030TA 6.5% 230.9 / 5.2 77D141E (GAT→GAA) PP_0891hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/32);  new base A (2/3);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCA  >  NC_002947/1033657‑1033951
                                                                                                                                                  |                                                                                                                                                    
gAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATgc                                                                                                                                                    >  4:295161/1‑149 (MQ=255)
     cacaGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCgcggc                                                                                                                                               >  2:129426/1‑149 (MQ=255)
          tGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCACCTGGAAGCGCCGCGCATc                                                                                                                                          >  4:124036/1‑149 (MQ=255)
           gAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTc                                                                                                                                          >  5:291652/1‑148 (MQ=255)
             aGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGAAGCGCCGCGCATCCa                                                                                                                                        <  5:245259/148‑1 (MQ=255)
                cacaGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCTAggtg                                                                                                                                    <  5:108169/149‑1 (MQ=255)
                 acaGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCACCTGGAAGCGCCGCGCATCCAggcgg                                                                                                                                   <  3:124036/149‑1 (MQ=255)
                     aTCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAc                                                                                                                               <  3:93876/149‑1 (MQ=255)
                           cTGCATCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGa                                                                                                                         <  4:207876/149‑1 (MQ=255)
                               aGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCct                                                                                                                     >  3:254083/1‑149 (MQ=255)
                                gCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGTGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCctc                                                                                                                    <  8:199393/149‑1 (MQ=255)
                                 ccGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCctct                                                                                                                   >  7:185490/1‑149 (MQ=255)
                                       aaTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGtgct                                                                                                             <  4:254083/149‑1 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtggt                                                                                                                                  <  3:271716/127‑1 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtggt                                                                                                                                  >  4:271716/1‑127 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtg                                                                                                                                    >  5:13600/1‑125 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAccc                                                                                                                             >  4:242930/1‑132 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAccc                                                                                                                             <  3:242930/132‑1 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGtgctg                                                                                                            >  6:8875/1‑149 (MQ=255)
                                        aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGAGGCGCTTCCAggtg                                                                                                                                    <  6:13600/125‑1 (MQ=255)
                                           aCCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTgcg                                                                                                          <  8:185490/148‑1 (MQ=255)
                                             cTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTgcgcgc                                                                                                       <  2:115828/149‑1 (MQ=255)
                                                           acaGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCTCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCt                                                                                         <  3:54873/149‑1 (MQ=255)
                                                             aGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGAAGCGCCGCGCATCCAggcgg                                                                                                                                   >  6:163820/1‑105 (MQ=255)
                                                             aGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGAAGCGCCGCGCATCCAggcgg                                                                                                                                   <  5:163820/105‑1 (MQ=255)
                                                               aTCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTg                                                                                            >  6:61457/1‑142 (MQ=255)
                                                               aTCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTg                                                                                            <  5:61457/142‑1 (MQ=255)
                                                                   aaTTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCg                                                                                 <  2:108792/149‑1 (MQ=255)
                                                                    aTTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCgg                                                                                >  8:279125/1‑149 (MQ=255)
                                                                     ttGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCggg                                                                               >  3:120784/1‑149 (MQ=255)
                                                                        cgcCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCt                                                                            <  4:120784/149‑1 (MQ=255)
                                                                                gCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGcc                                                                    >  3:201021/1‑149 (MQ=255)
                                                                                gCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGcc                                                                    <  1:129426/149‑1 (MQ=255)
                                                                                    aCGGTCACCCTGGATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCGTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGTGCTGGAAAGCCTgc                                                                 <  6:291652/148‑1 (MQ=255)
                                                                                       gTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCa                                                              >  8:275523/1‑148 (MQ=255)
                                                                                        acACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCAAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTAGCCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAg                                                            >  2:307442/2‑149 (MQ=255)
                                                                                         cACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGt                                                           >  5:159629/1‑149 (MQ=255)
                                                                                          aCCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCgg                                                                                >  6:227765/1‑127 (MQ=255)
                                                                                          aCCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCgg                                                                                <  5:227765/127‑1 (MQ=255)
                                                                                            ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                         >  2:311841/1‑148 (MQ=255)
                                                                                            ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                         <  1:311841/148‑1 (MQ=255)
                                                                                            ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCg                                                        >  4:41018/1‑149 (MQ=255)
                                                                                             cTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGc                                                       <  7:279125/149‑1 (MQ=255)
                                                                                                cATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCa                                                     <  3:295161/148‑1 (MQ=255)
                                                                                                    gCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGtt                                                 >  5:64638/1‑148 (MQ=255)
                                                                                                            cacaGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCAt                                        >  2:159248/1‑149 (MQ=255)
                                                                                                               aGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCaa                                     >  7:166352/1‑149 (MQ=255)
                                                                                                               aGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCaa                                     >  2:333746/1‑149 (MQ=255)
                                                                                                                 cTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                             <  2:129628/123‑1 (MQ=255)
                                                                                                                 cTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                             >  1:129628/1‑123 (MQ=255)
                                                                                                                    cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCt                                                                   >  2:196915/1‑114 (MQ=255)
                                                                                                                    cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                             <  4:162623/120‑1 (MQ=255)
                                                                                                                    cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                             >  3:162623/1‑120 (MQ=255)
                                                                                                                    cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATATTGGCTTGCTGCCGGGGCTGGAAAGCCt                                                                   <  1:196915/114‑1 (MQ=255)
                                                                                                                     aaTGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTc                               <  6:159629/149‑1 (MQ=255)
                                                                                                                         tacTGTTGCTCGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTa                           >  3:219542/3‑149 (MQ=255)
                                                                                                                           cTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAgg                                                   <  5:57269/123‑1 (MQ=255)
                                                                                                                           cTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAgg                                                   >  6:57269/1‑123 (MQ=255)
                                                                                                                              ttGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCg                        <  7:275523/147‑1 (MQ=255)
                                                                                                                              ttGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGt                      >  1:183647/1‑149 (MQ=255)
                                                                                                                                gCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGt                      >  8:182622/1‑147 (MQ=255)
                                                                                                                                 cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                         <  1:319362/111‑1 (MQ=255)
                                                                                                                                 cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                         >  2:319362/1‑111 (MQ=255)
                                                                                                                                 cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAg                   >  4:66425/1‑149 (MQ=255)
                                                                                                                                 cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTAGGTACCGGTTa                    <  1:159248/148‑1 (MQ=255)
                                                                                                                                  tGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGGCGGTACCGGTTAgc                  >  7:296125/1‑149 (MQ=255)
                                                                                                                                  tGGCCACCCCCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgc                  >  3:259078/1‑149 (MQ=255)
                                                                                                                                    gCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgcgc                >  8:30589/1‑149 (MQ=255)
                                                                                                                                    gCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgcgc                >  8:74786/1‑149 (MQ=255)
                                                                                                                                     ccACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGcgcg               <  4:201021/149‑1 (MQ=255)
                                                                                                                                      cACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCa                                         >  1:80368/1‑122 (MQ=255)
                                                                                                                                      cACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCa                                         <  2:80368/122‑1 (MQ=255)
                                                                                                                                       aCCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGt             >  2:16536/1‑149 (MQ=255)
                                                                                                                                         ccGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGc           <  3:41018/149‑1 (MQ=255)
                                                                                                                                         ccGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGc           >  4:113003/1‑149 (MQ=255)
                                                                                                                                          cGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCt          >  3:205306/1‑149 (MQ=255)
                                                                                                                                             cTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGAt       <  3:66425/149‑1 (MQ=255)
                                                                                                                                                  tGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCa  <  1:333746/149‑1 (MQ=255)
                                                                                                                                                  tGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCa  <  6:64638/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCA  >  NC_002947/1033657‑1033951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑