Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,033,808 G→C 6.4% R143P (CGG→CCG)  PP_0891 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,033,8080GC 6.4% 247.0 / 4.4 77R143P (CGG→CCG) PP_0891hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (38/34);  new base C (2/3);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCACTG  >  NC_002947/1033662‑1033954
                                                                                                                                                  |                                                                                                                                                  
cacaGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCgcggc                                                                                                                                                  >  2:129426/1‑149 (MQ=255)
     tGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCACCTGGAAGCGCCGCGCATc                                                                                                                                             >  4:124036/1‑149 (MQ=255)
      gAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTc                                                                                                                                             >  5:291652/1‑148 (MQ=255)
        aGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGAAGCGCCGCGCATCCa                                                                                                                                           <  5:245259/148‑1 (MQ=255)
           cacaGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCTAggtg                                                                                                                                       <  5:108169/149‑1 (MQ=255)
            acaGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCACCTGGAAGCGCCGCGCATCCAggcgg                                                                                                                                      <  3:124036/149‑1 (MQ=255)
                aTCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAc                                                                                                                                  <  3:93876/149‑1 (MQ=255)
                      cTGCATCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGa                                                                                                                            <  4:207876/149‑1 (MQ=255)
                          aGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCct                                                                                                                        >  3:254083/1‑149 (MQ=255)
                           gCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGTGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCctc                                                                                                                       <  8:199393/149‑1 (MQ=255)
                            ccGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCctct                                                                                                                      >  7:185490/1‑149 (MQ=255)
                                  aaTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGtgct                                                                                                                <  4:254083/149‑1 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtggt                                                                                                                                     <  3:271716/127‑1 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtggt                                                                                                                                     >  4:271716/1‑127 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAggtg                                                                                                                                       >  5:13600/1‑125 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAccc                                                                                                                                >  4:242930/1‑132 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGAccc                                                                                                                                <  3:242930/132‑1 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGtgctg                                                                                                               >  6:8875/1‑149 (MQ=255)
                                   aTGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGAGGCGCTTCCAggtg                                                                                                                                       <  6:13600/125‑1 (MQ=255)
                                      aCCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTgcg                                                                                                             <  8:185490/148‑1 (MQ=255)
                                        cTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTgcgcgc                                                                                                          <  2:115828/149‑1 (MQ=255)
                                                      acaGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCTCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCt                                                                                            <  3:54873/149‑1 (MQ=255)
                                                        aGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGAAGCGCCGCGCATCCAggcgg                                                                                                                                      >  6:163820/1‑105 (MQ=255)
                                                        aGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGAAGCGCCGCGCATCCAggcgg                                                                                                                                      <  5:163820/105‑1 (MQ=255)
                                                          aTCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTg                                                                                               >  6:61457/1‑142 (MQ=255)
                                                          aTCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTg                                                                                               <  5:61457/142‑1 (MQ=255)
                                                              aaTTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCg                                                                                    <  2:108792/149‑1 (MQ=255)
                                                               aTTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCgg                                                                                   >  8:279125/1‑149 (MQ=255)
                                                                ttGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCggg                                                                                  >  3:120784/1‑149 (MQ=255)
                                                                   cgcCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCt                                                                               <  4:120784/149‑1 (MQ=255)
                                                                           gCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGcc                                                                       >  3:201021/1‑149 (MQ=255)
                                                                           gCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGcc                                                                       <  1:129426/149‑1 (MQ=255)
                                                                               aCGGTCACCCTGGATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCGTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGTGCTGGAAAGCCTgc                                                                    <  6:291652/148‑1 (MQ=255)
                                                                                  gTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCa                                                                 >  8:275523/1‑148 (MQ=255)
                                                                                   acACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCAAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTAGCCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAg                                                               >  2:307442/2‑149 (MQ=255)
                                                                                    cACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGt                                                              >  5:159629/1‑149 (MQ=255)
                                                                                     aCCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCgg                                                                                   <  5:227765/127‑1 (MQ=255)
                                                                                     aCCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCgg                                                                                   >  6:227765/1‑127 (MQ=255)
                                                                                       ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                            >  2:311841/1‑148 (MQ=255)
                                                                                       ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                            <  1:311841/148‑1 (MQ=255)
                                                                                       ccTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCg                                                           >  4:41018/1‑149 (MQ=255)
                                                                                        cTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGc                                                          <  7:279125/149‑1 (MQ=255)
                                                                                           cATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCa                                                        <  3:295161/148‑1 (MQ=255)
                                                                                               gCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGtt                                                    >  5:64638/1‑148 (MQ=255)
                                                                                                       cacaGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCAt                                           >  2:159248/1‑149 (MQ=255)
                                                                                                          aGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCaa                                        >  2:333746/1‑149 (MQ=255)
                                                                                                          aGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCaa                                        >  7:166352/1‑149 (MQ=255)
                                                                                                            cTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                                <  2:129628/123‑1 (MQ=255)
                                                                                                            cTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                                >  1:129628/1‑123 (MQ=255)
                                                                                                               cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCt                                                                      >  2:196915/1‑114 (MQ=255)
                                                                                                               cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                                <  4:162623/120‑1 (MQ=255)
                                                                                                               cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCaa                                                                >  3:162623/1‑120 (MQ=255)
                                                                                                               cAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATATTGGCTTGCTGCCGGGGCTGGAAAGCCt                                                                      <  1:196915/114‑1 (MQ=255)
                                                                                                                aaTGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTc                                  <  6:159629/149‑1 (MQ=255)
                                                                                                                    tacTGTTGCTCGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTa                              >  3:219542/3‑149 (MQ=255)
                                                                                                                      cTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAgg                                                      >  6:57269/1‑123 (MQ=255)
                                                                                                                      cTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAgg                                                      <  5:57269/123‑1 (MQ=255)
                                                                                                                         ttGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCg                           <  7:275523/147‑1 (MQ=255)
                                                                                                                         ttGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGt                         >  1:183647/1‑149 (MQ=255)
                                                                                                                           gCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGt                         >  8:182622/1‑147 (MQ=255)
                                                                                                                            cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                            <  1:319362/111‑1 (MQ=255)
                                                                                                                            cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTc                                                            >  2:319362/1‑111 (MQ=255)
                                                                                                                            cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAg                      >  4:66425/1‑149 (MQ=255)
                                                                                                                            cTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTAGGTACCGGTTa                       <  1:159248/148‑1 (MQ=255)
                                                                                                                             tGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGGCGGTACCGGTTAgc                     >  7:296125/1‑149 (MQ=255)
                                                                                                                             tGGCCACCCCCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgc                     >  3:259078/1‑149 (MQ=255)
                                                                                                                               gCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgcgc                   >  8:30589/1‑149 (MQ=255)
                                                                                                                               gCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAgcgc                   >  8:74786/1‑149 (MQ=255)
                                                                                                                                ccACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGcgcg                  <  4:201021/149‑1 (MQ=255)
                                                                                                                                 cACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCa                                            >  1:80368/1‑122 (MQ=255)
                                                                                                                                 cACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCa                                            <  2:80368/122‑1 (MQ=255)
                                                                                                                                  aCCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGt                >  2:16536/1‑149 (MQ=255)
                                                                                                                                    ccGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGc              <  3:41018/149‑1 (MQ=255)
                                                                                                                                    ccGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGc              >  4:113003/1‑149 (MQ=255)
                                                                                                                                     cGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCt             >  3:205306/1‑149 (MQ=255)
                                                                                                                                        cTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGAt          <  3:66425/149‑1 (MQ=255)
                                                                                                                                             tGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCa     <  1:333746/149‑1 (MQ=255)
                                                                                                                                             tGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCa     <  6:64638/149‑1 (MQ=255)
                                                                                                                                                gcggcgCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCACTg  >  5:223862/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACGGTCACCCTGCATGGCCAGTCGCACAGCTACAATGTCCTGTTGCTGGCCACCCGCCTGGATGCGCGGCGCTTCCAGGTGGTGACCCTTGAGCCTCTGGTGCTGCGCGCCGAAGATTTTGGCTTGCTGCCGGGGCTGGAAAGCCTGCGCAAGTTCGCCAGGTTGAAGTCCATCAACCCGTCGGTACCGGTTAGCGCGGTGCTGATCTTCACTG  >  NC_002947/1033662‑1033954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑