Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,155,458 C→G 6.3% P206A (CCG→GCG)  PP_1013 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,155,4580CG 6.3% 190.7 / 3.5 63P206A (CCG→GCG) PP_1013sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base G (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

CCGCGCTCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGC  >  NC_002947/1155311‑1155606
                                                                                                                                                   |                                                                                                                                                     
ccGCGCTCATGGGCCCATTGTTGGCTGAACTTGGAGCCGCTGAAACCGCCTGTAGTGGTCACGCAGATGCGGTTGGGCGAGGGCGAATGGTTGTACATCGCCTGGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGcg                                                                                                                                                      <  1:125989/149‑2 (MQ=255)
         tGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCcagca                                                                                                                                             <  1:226943/149‑1 (MQ=255)
          gggCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCcag                                                                                                                                               >  5:18464/1‑146 (MQ=255)
              ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCgcgc                                                                                                                                                  >  5:10389/1‑139 (MQ=255)
              ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCgcgc                                                                                                                                                  <  6:10389/139‑1 (MQ=255)
              ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCcag                                                                                                                                               >  6:267153/1‑142 (MQ=255)
               cATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCgg                                                                                                                                       >  7:207496/1‑149 (MQ=255)
               cATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGCGCGAGGGCGAATGGAAGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCgg                                                                                                                                       >  1:82867/1‑149 (MQ=255)
                attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGc                                                                                                                                      <  6:58713/149‑1 (MQ=255)
                attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGc                                                                                                                                      <  1:216386/149‑1 (MQ=255)
                attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGc                                                                                                                                      <  6:327600/149‑1 (MQ=255)
                   attCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCtt                                                                                                                                    >  2:181352/1‑148 (MQ=255)
                       gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                                <  3:321125/148‑1 (MQ=255)
                       gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                                <  8:131373/148‑1 (MQ=255)
                           aCCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                           <  5:39235/149‑1 (MQ=255)
                            ccTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                           >  6:271775/1‑148 (MQ=255)
                                     cGCTGAAACCCCCGGTACTGGTAACGCAGAAACGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTAAACGGGGCTGGAAGCCGAACGCATGCCGCGCAAGCAGAACGGCTTCATCGTGCTGACCACGGcc                                                                                                                 >  7:51290/1‑149 (MQ=255)
                                         gAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgc                                                                                                             >  3:219577/1‑149 (MQ=255)
                                          aaaCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCTCGGCCttg                                                                                                              <  1:181352/147‑1 (MQ=255)
                                           aaCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctg                                                                                                           >  7:128255/1‑149 (MQ=255)
                                            aCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgt                                                                                                          >  7:290523/1‑149 (MQ=255)
                                             cccccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                         >  1:62968/1‑149 (MQ=255)
                                             cccccGGTACTGCTCACGCAGATCCGCTTGGGCCAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCTTACACGGGGCTGGAAGCAGAACGCCTGCCGCGCCAGCAGAGCGGCTTCATCGTGCTGACAACGGCCttgctgtt                                                                                                         >  4:192193/1‑149 (MQ=255)
                                                   gTACTGGTCACGGAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAAGCGTAGTTGGGGCTGGATGCCGAACGCCTGCCGCTCCAGCAGATGGGCTTCATCGTGCTGACCACGGCCTTgctgttgctgtt                                                                                                   <  3:192193/149‑1 (MQ=255)
                                                          tCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGGACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCCTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGc                                                                                            <  4:54832/149‑1 (MQ=255)
                                                              gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGc                                                                                        >  6:4548/1‑149 (MQ=255)
                                                              gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGc                                                                                        >  8:319300/1‑149 (MQ=255)
                                                                aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                         >  8:280946/1‑130 (MQ=255)
                                                                aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                         <  7:280946/130‑1 (MQ=255)
                                                                   tCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                   <  8:207496/149‑1 (MQ=255)
                                                                       cTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACt                                                                               <  5:267153/149‑1 (MQ=255)
                                                                          gggcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTgg                                                                             <  3:56696/148‑1 (MQ=255)
                                                                            gcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                   >  5:118954/1‑140 (MQ=255)
                                                                            gcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                   <  6:118954/140‑1 (MQ=255)
                                                                                  gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                    <  6:18464/149‑1 (MQ=255)
                                                                                  gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTg                                                                     <  8:290523/148‑1 (MQ=255)
                                                                                   tgaATGGTTGTAGATCGCGTCGGTGTTGCCCGTTCCGTTGACTGGTCTGGAAGCCGATCGCCTGCCGCGCCAGGAGATCGGCTTCATCGTGCTGACGACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                                   <  5:271775/148‑1 (MQ=255)
                                                                                   cgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                                   <  8:128255/149‑1 (MQ=255)
                                                                                         gTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTg                                                              >  8:153451/1‑148 (MQ=255)
                                                                                            gTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGt                                                                                    >  1:269658/1‑123 (MQ=255)
                                                                                            gTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGt                                                                                    <  2:269658/123‑1 (MQ=255)
                                                                                                 tCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTg                                                     <  2:82867/149‑1 (MQ=255)
                                                                                                    ccTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCAt                                                                                                <  5:150980/103‑1 (MQ=255)
                                                                                                    ccTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCAt                                                                                                >  6:150980/1‑103 (MQ=255)
                                                                                                         cTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                    <  8:24426/126‑1 (MQ=255)
                                                                                                         cTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                    >  7:24426/1‑126 (MQ=255)
                                                                                                            ttGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                         <  7:153451/149‑1 (MQ=255)
                                                                                                               cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGccc                                       >  8:113695/1‑148 (MQ=255)
                                                                                                               cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGAGCGG‑GCCGccc                                       >  7:183084/1‑148 (MQ=255)
                                                                                                                 cGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGccc                                       >  1:213362/1‑146 (MQ=255)
                                                                                                                     ccGTACACGCGGCAGGAAGCCGAACGCCTGCCGCGCCACCAGATCGCGTTCAACGTGCAGACCACGGCCTTGCTGTTGCTCATCATCGGCTTGCAGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGAGG‑GCCGCCCGAGAGAt                                >  1:249896/1‑149 (MQ=255)
                                                                                                                       gTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTAGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGt                              >  4:88114/1‑149 (MQ=255)
                                                                                                                           acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                         <  6:219235/134‑1 (MQ=255)
                                                                                                                           acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                         >  5:219235/1‑134 (MQ=255)
                                                                                                                           acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGATGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCt                          >  4:335779/1‑149 (MQ=255)
                                                                                                                             ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                        <  7:113695/149‑1 (MQ=255)
                                                                                                                             ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                        <  8:183084/149‑1 (MQ=255)
                                                                                                                               ggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACAGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGt                      <  4:219577/149‑1 (MQ=255)
                                                                                                                                 gatgAAGCCGATCTGCTGGTGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGc                    >  3:195211/4‑149 (MQ=38)
                                                                                                                                 gatgAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGc                    >  2:51300/4‑149 (MQ=38)
                                                                                                                                    gAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGa                 <  3:88114/149‑1 (MQ=255)
                                                                                                                                       gCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGAtgt              <  2:213362/149‑1 (MQ=255)
                                                                                                                                                cTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGAGCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGgc           <  1:51300/144‑1 (MQ=255)
                                                                                                                                                cTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGt     >  6:141205/1‑149 (MQ=255)
                                                                                                                                                   ccGCGCCAGCAGATCGGCTTCATCGTGCTGCCCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGc  >  7:154148/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCGCGCTCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGC  >  NC_002947/1155311‑1155606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑