Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,155,463 C→G 6.4% R207R (CGC→CGG)  PP_1013 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,155,4630CG 6.4% 185.8 / 3.3 62R207R (CGC→CGG) PP_1013sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base G (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

TGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGAA  >  NC_002947/1155320‑1155610
                                                                                                                                               |                                                                                                                                                    
tGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCcagca                                                                                                                                                 <  1:226943/149‑1 (MQ=255)
 gggCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCcag                                                                                                                                                   >  5:18464/1‑146 (MQ=255)
     ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCgcgc                                                                                                                                                      >  5:10389/1‑139 (MQ=255)
     ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCgcgc                                                                                                                                                      <  6:10389/139‑1 (MQ=255)
     ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCcag                                                                                                                                                   >  6:267153/1‑142 (MQ=255)
      cATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCgg                                                                                                                                           >  7:207496/1‑149 (MQ=255)
      cATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGCGCGAGGGCGAATGGAAGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCgg                                                                                                                                           >  1:82867/1‑149 (MQ=255)
       attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGc                                                                                                                                          <  6:58713/149‑1 (MQ=255)
       attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGc                                                                                                                                          <  1:216386/149‑1 (MQ=255)
       attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGc                                                                                                                                          <  6:327600/149‑1 (MQ=255)
          attCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCtt                                                                                                                                        >  2:181352/1‑148 (MQ=255)
              gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                                    <  8:131373/148‑1 (MQ=255)
              gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                                    <  3:321125/148‑1 (MQ=255)
                  aCCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                               <  5:39235/149‑1 (MQ=255)
                   ccTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                               >  6:271775/1‑148 (MQ=255)
                            cGCTGAAACCCCCGGTACTGGTAACGCAGAAACGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTAAACGGGGCTGGAAGCCGAACGCATGCCGCGCAAGCAGAACGGCTTCATCGTGCTGACCACGGcc                                                                                                                     >  7:51290/1‑149 (MQ=255)
                                gAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgc                                                                                                                 >  3:219577/1‑149 (MQ=255)
                                 aaaCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCTCGGCCttg                                                                                                                  <  1:181352/147‑1 (MQ=255)
                                  aaCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctg                                                                                                               >  7:128255/1‑149 (MQ=255)
                                   aCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgt                                                                                                              >  7:290523/1‑149 (MQ=255)
                                    cccccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                             >  1:62968/1‑149 (MQ=255)
                                    cccccGGTACTGCTCACGCAGATCCGCTTGGGCCAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCTTACACGGGGCTGGAAGCAGAACGCCTGCCGCGCCAGCAGAGCGGCTTCATCGTGCTGACAACGGCCttgctgtt                                                                                                             >  4:192193/1‑149 (MQ=255)
                                          gTACTGGTCACGGAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAAGCGTAGTTGGGGCTGGATGCCGAACGCCTGCCGCTCCAGCAGATGGGCTTCATCGTGCTGACCACGGCCTTgctgttgctgtt                                                                                                       <  3:192193/149‑1 (MQ=255)
                                                 tCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGGACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCCTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGc                                                                                                <  4:54832/149‑1 (MQ=255)
                                                     gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGc                                                                                            >  6:4548/1‑149 (MQ=255)
                                                     gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGc                                                                                            >  8:319300/1‑149 (MQ=255)
                                                       aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                             >  8:280946/1‑130 (MQ=255)
                                                       aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                             <  7:280946/130‑1 (MQ=255)
                                                          tCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                       <  8:207496/149‑1 (MQ=255)
                                                              cTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACt                                                                                   <  5:267153/149‑1 (MQ=255)
                                                                 gggcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTgg                                                                                 <  3:56696/148‑1 (MQ=255)
                                                                   gcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                       >  5:118954/1‑140 (MQ=255)
                                                                   gcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                       <  6:118954/140‑1 (MQ=255)
                                                                         gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                        <  6:18464/149‑1 (MQ=255)
                                                                         gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTg                                                                         <  8:290523/148‑1 (MQ=255)
                                                                          tgaATGGTTGTAGATCGCGTCGGTGTTGCCCGTTCCGTTGACTGGTCTGGAAGCCGATCGCCTGCCGCGCCAGGAGATCGGCTTCATCGTGCTGACGACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                                       <  5:271775/148‑1 (MQ=255)
                                                                          cgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                                       <  8:128255/149‑1 (MQ=255)
                                                                                gTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTg                                                                  >  8:153451/1‑148 (MQ=255)
                                                                                   gTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGt                                                                                        <  2:269658/123‑1 (MQ=255)
                                                                                   gTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGt                                                                                        >  1:269658/1‑123 (MQ=255)
                                                                                        tCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTg                                                         <  2:82867/149‑1 (MQ=255)
                                                                                           ccTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCAt                                                                                                    <  5:150980/103‑1 (MQ=255)
                                                                                           ccTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCAt                                                                                                    >  6:150980/1‑103 (MQ=255)
                                                                                                cTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                        <  8:24426/126‑1 (MQ=255)
                                                                                                cTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                        >  7:24426/1‑126 (MQ=255)
                                                                                                   ttGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                             <  7:153451/149‑1 (MQ=255)
                                                                                                      cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGccc                                           >  8:113695/1‑148 (MQ=255)
                                                                                                      cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGAGCGG‑GCCGccc                                           >  7:183084/1‑148 (MQ=255)
                                                                                                        cGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGccc                                           >  1:213362/1‑146 (MQ=255)
                                                                                                            ccGTACACGCGGCAGGAAGCCGAACGCCTGCCGCGCCACCAGATCGCGTTCAACGTGCAGACCACGGCCTTGCTGTTGCTCATCATCGGCTTGCAGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGAGG‑GCCGCCCGAGAGAt                                    >  1:249896/1‑149 (MQ=255)
                                                                                                              gTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTAGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGt                                  >  4:88114/1‑149 (MQ=255)
                                                                                                                  acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                             <  6:219235/134‑1 (MQ=255)
                                                                                                                  acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                             >  5:219235/1‑134 (MQ=255)
                                                                                                                  acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGATGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCt                              >  4:335779/1‑149 (MQ=255)
                                                                                                                    ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                            <  7:113695/149‑1 (MQ=255)
                                                                                                                    ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                            <  8:183084/149‑1 (MQ=255)
                                                                                                                      ggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACAGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGt                          <  4:219577/149‑1 (MQ=255)
                                                                                                                        gatgAAGCCGATCTGCTGGTGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGc                        >  3:195211/4‑149 (MQ=38)
                                                                                                                        gatgAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGc                        >  2:51300/4‑149 (MQ=38)
                                                                                                                           gAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGa                     <  3:88114/149‑1 (MQ=255)
                                                                                                                              gCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGAtgt                  <  2:213362/149‑1 (MQ=255)
                                                                                                                                       cTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGAGCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGgc               <  1:51300/144‑1 (MQ=255)
                                                                                                                                       cTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGt         >  6:141205/1‑149 (MQ=255)
                                                                                                                                          ccGCGCCAGCAGATCGGCTTCATCGTGCTGCCCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGc      >  7:154148/1‑149 (MQ=255)
                                                                                                                                              gcCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGaa  <  5:4548/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGAA  >  NC_002947/1155320‑1155610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑