Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,155,470 A→T 6.6% I210F (ATC→TTC)  PP_1013 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,155,4700AT 6.6% 176.4 / 2.7 61I210F (ATC→TTC) PP_1013sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base T (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

CATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGAAG  >  NC_002947/1155326‑1155611
                                                                                                                                                |                                                                                                                                              
cATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCgg                                                                                                                                            >  7:207496/1‑149 (MQ=255)
cATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGCGCGAGGGCGAATGGAAGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCgg                                                                                                                                            >  1:82867/1‑149 (MQ=255)
 attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGc                                                                                                                                           <  6:58713/149‑1 (MQ=255)
 attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGc                                                                                                                                           <  1:216386/149‑1 (MQ=255)
 attattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGc                                                                                                                                           <  6:327600/149‑1 (MQ=255)
    attCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCtt                                                                                                                                         >  2:181352/1‑148 (MQ=255)
        gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                                     <  3:321125/148‑1 (MQ=255)
        gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                                     <  8:131373/148‑1 (MQ=255)
            aCCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                                <  5:39235/149‑1 (MQ=255)
             ccTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                                >  6:271775/1‑148 (MQ=255)
                      cGCTGAAACCCCCGGTACTGGTAACGCAGAAACGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTAAACGGGGCTGGAAGCCGAACGCATGCCGCGCAAGCAGAACGGCTTCATCGTGCTGACCACGGcc                                                                                                                      >  7:51290/1‑149 (MQ=255)
                          gAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgc                                                                                                                  >  3:219577/1‑149 (MQ=255)
                           aaaCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCTCGGCCttg                                                                                                                   <  1:181352/147‑1 (MQ=255)
                            aaCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctg                                                                                                                >  7:128255/1‑149 (MQ=255)
                             aCCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgt                                                                                                               >  7:290523/1‑149 (MQ=255)
                              cccccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                              >  1:62968/1‑149 (MQ=255)
                              cccccGGTACTGCTCACGCAGATCCGCTTGGGCCAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCTTACACGGGGCTGGAAGCAGAACGCCTGCCGCGCCAGCAGAGCGGCTTCATCGTGCTGACAACGGCCttgctgtt                                                                                                              >  4:192193/1‑149 (MQ=255)
                                    gTACTGGTCACGGAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAAGCGTAGTTGGGGCTGGATGCCGAACGCCTGCCGCTCCAGCAGATGGGCTTCATCGTGCTGACCACGGCCTTgctgttgctgtt                                                                                                        <  3:192193/149‑1 (MQ=255)
                                           tCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGGACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCCTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGc                                                                                                 <  4:54832/149‑1 (MQ=255)
                                               gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGc                                                                                             >  6:4548/1‑149 (MQ=255)
                                               gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGc                                                                                             >  8:319300/1‑149 (MQ=255)
                                                 aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                              <  7:280946/130‑1 (MQ=255)
                                                 aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                                              >  8:280946/1‑130 (MQ=255)
                                                    tCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                        <  8:207496/149‑1 (MQ=255)
                                                        cTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACt                                                                                    <  5:267153/149‑1 (MQ=255)
                                                           gggcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTgg                                                                                  <  3:56696/148‑1 (MQ=255)
                                                             gcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                        <  6:118954/140‑1 (MQ=255)
                                                             gcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                                        >  5:118954/1‑140 (MQ=255)
                                                                   gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                         <  6:18464/149‑1 (MQ=255)
                                                                   gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTg                                                                          <  8:290523/148‑1 (MQ=255)
                                                                    tgaATGGTTGTAGATCGCGTCGGTGTTGCCCGTTCCGTTGACTGGTCTGGAAGCCGATCGCCTGCCGCGCCAGGAGATCGGCTTCATCGTGCTGACGACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                                        <  5:271775/148‑1 (MQ=255)
                                                                    cgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                                        <  8:128255/149‑1 (MQ=255)
                                                                          gTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTg                                                                   >  8:153451/1‑148 (MQ=255)
                                                                             gTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGt                                                                                         >  1:269658/1‑123 (MQ=255)
                                                                             gTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGt                                                                                         <  2:269658/123‑1 (MQ=255)
                                                                                  tCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTg                                                          <  2:82867/149‑1 (MQ=255)
                                                                                     ccTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCAt                                                                                                     <  5:150980/103‑1 (MQ=255)
                                                                                     ccTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCAt                                                                                                     >  6:150980/1‑103 (MQ=255)
                                                                                          cTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                         <  8:24426/126‑1 (MQ=255)
                                                                                          cTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTgg                                                                         >  7:24426/1‑126 (MQ=255)
                                                                                             ttGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                              <  7:153451/149‑1 (MQ=255)
                                                                                                cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGccc                                            >  8:113695/1‑148 (MQ=255)
                                                                                                cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGAGCGG‑GCCGccc                                            >  7:183084/1‑148 (MQ=255)
                                                                                                  cGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGccc                                            >  1:213362/1‑146 (MQ=255)
                                                                                                      ccGTACACGCGGCAGGAAGCCGAACGCCTGCCGCGCCACCAGATCGCGTTCAACGTGCAGACCACGGCCTTGCTGTTGCTCATCATCGGCTTGCAGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGAGG‑GCCGCCCGAGAGAt                                     >  1:249896/1‑149 (MQ=255)
                                                                                                        gTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTAGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGt                                   >  4:88114/1‑149 (MQ=255)
                                                                                                            acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                              <  6:219235/134‑1 (MQ=255)
                                                                                                            acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑gccgc                                              >  5:219235/1‑134 (MQ=255)
                                                                                                            acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGATGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCt                               >  4:335779/1‑149 (MQ=255)
                                                                                                              ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                             <  7:113695/149‑1 (MQ=255)
                                                                                                              ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                             <  8:183084/149‑1 (MQ=255)
                                                                                                                ggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACAGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGt                           <  4:219577/149‑1 (MQ=255)
                                                                                                                  gatgAAGCCGATCTGCTGGTGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGc                         >  3:195211/4‑149 (MQ=38)
                                                                                                                  gatgAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGc                         >  2:51300/4‑149 (MQ=38)
                                                                                                                     gAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGa                      <  3:88114/149‑1 (MQ=255)
                                                                                                                        gCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGAtgt                   <  2:213362/149‑1 (MQ=255)
                                                                                                                                 cTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGAGCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGgc                <  1:51300/144‑1 (MQ=255)
                                                                                                                                 cTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGt          >  6:141205/1‑149 (MQ=255)
                                                                                                                                    ccGCGCCAGCAGATCGGCTTCATCGTGCTGCCCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGc       >  7:154148/1‑149 (MQ=255)
                                                                                                                                        gcCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGaa   <  5:4548/149‑1 (MQ=255)
                                                                                                                                          cagcagATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGAAg  >  4:241090/1‑148 (MQ=255)
                                                                                                                                                aTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                             <  4:248967/115‑1 (MQ=255)
                                                                                                                                                aTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTgg                             >  3:248967/1‑115 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGG‑GCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGCGCCAGTGGCTGAAG  >  NC_002947/1155326‑1155611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑