| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,178,754 | T→A | 10.1% | intergenic (+59/+35) | guaA → / ← PP_1033 | glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,178,754 | 0 | T | A | 10.1% | 166.8 / 7.1 | 59 | intergenic (+59/+35) | guaA/PP_1033 | glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (22/31); new base A (2/4); total (24/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTT > NC_002947/1178608‑1178896 | cGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTg > 3:88982/1‑149 (MQ=255) ggTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGc > 1:169696/1‑149 (MQ=255) ggTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGc < 2:269418/149‑1 (MQ=255) tCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGCCGGGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGAGCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCgg < 6:343868/149‑1 (MQ=255) tATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCt < 7:273373/149‑1 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt > 1:66992/1‑149 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt < 6:188000/149‑1 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt < 6:324358/149‑1 (MQ=255) tcaACGAAATCGACGTTATCTCTCGCGTTACCTACGACTTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCTTTCTg < 6:349824/149‑1 (MQ=255) tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTg > 7:48693/1‑149 (MQ=255) tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCTTTCTg < 5:5149/149‑1 (MQ=255) cGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTc < 8:219670/149‑1 (MQ=255) cGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttcgt < 4:44815/148‑1 (MQ=255) gcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTg < 2:66992/147‑1 (MQ=255) gcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGc < 2:326850/148‑1 (MQ=255) gcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCt > 6:232568/1‑149 (MQ=255) cgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCtt < 6:307976/149‑1 (MQ=255) tACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTg > 7:141763/1‑128 (MQ=255) tACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTg < 8:141763/128‑1 (MQ=255) aCCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGACTTTCTGGTTCTTGTTCGTTCAGATTGCTTAttctt > 7:235769/1‑149 (MQ=255) ccTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTAttcttc < 8:49647/149‑1 (MQ=255) acgacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTgg > 5:126133/1‑149 (MQ=255) gAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGc < 3:9826/148‑1 (MQ=255) aGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCa > 8:21226/1‑148 (MQ=255) aaGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGACaaa > 5:331765/1‑147 (MQ=255) aGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAg < 7:180573/149‑1 (MQ=255) cACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTgg > 3:322168/1‑149 (MQ=255) cTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTa < 2:169696/149‑1 (MQ=255) tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAgg < 5:232568/148‑1 (MQ=255) tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCACATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGc > 5:50426/1‑128 (MQ=255) tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCACATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGc < 6:50426/128‑1 (MQ=255) gTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTta < 7:160555/149‑1 (MQ=255) tGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat < 8:48693/149‑1 (MQ=255) ggAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGACTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtata < 8:235769/148‑1 (MQ=255) ggACTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCACGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGCTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCATTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAg > 6:302662/1‑149 (MQ=255) gAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCt < 4:262245/130‑1 (MQ=255) gAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCt > 3:262245/1‑130 (MQ=255) tGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGTTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGa > 4:283605/1‑147 (MQ=255) gggtgccagccTGGGCTGGCTACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagcagc > 3:206162/5‑149 (MQ=255) ggTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGcc < 4:88982/149‑1 (MQ=255) gTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCt < 3:38927/149‑1 (MQ=255) tgccagccTGGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGc > 6:115254/2‑149 (MQ=255) ccagccCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct < 3:116806/95‑1 (MQ=255) ccagccCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct > 4:116806/1‑95 (MQ=255) cagccCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct < 6:91878/94‑1 (MQ=255) cTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGTTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGa < 3:283605/149‑1 (MQ=255) tGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGAt < 4:322168/149‑1 (MQ=255) ccGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTc > 8:135877/1‑149 (MQ=255) ggATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagcagc > 7:221834/1‑117 (MQ=255) ggATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagcagc < 8:221834/117‑1 (MQ=255) ggATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGACAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTc < 6:331765/149‑1 (MQ=255) acacCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcg > 3:161192/1‑149 (MQ=255) cAGAAAGCCCGCAATCATGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGtt > 6:11424/1‑149 (MQ=255) gAAAGCCCGCAATCATGCGTGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGTCAAATAGCGCTTTCTGGTAGGCGTtat < 7:155253/96‑1 (MQ=255) gAAAGCCCGCAATCATGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGAAAATAGCGCTTTCTGGTAGGCGTtat > 8:155253/1‑96 (MQ=38) aaGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTgggg < 4:161192/149‑1 (MQ=255) aaGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTggg < 7:135877/148‑1 (MQ=255) gCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCtt < 4:206162/149‑1 (MQ=255) gCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCtt > 4:303410/1‑149 (MQ=255) | CGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTT > NC_002947/1178608‑1178896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |