Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,189,979 C→A 7.8% R41L (CGG→CTG)  tatB‑I ← Sec‑independent protein translocase TatB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,189,9790CA 7.8% 158.8 / 3.7 51R41L (CGG→CTG) tatB‑ISec‑independent protein translocase TatB
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base A (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CACGGCCGTGGCCGCATCATTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCGGTCCGCTTGAGATTG  >  NC_002947/1189834‑1190126
                                                                                                                                                 |                                                                                                                                                    
caCGGCCGTGGCCGCATCATTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGgc                                                                                                                                                   <  8:255649/149‑1 (MQ=255)
   ggCCGTGGCCGCATCATTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGgcgcg                                                                                                                                                <  8:14220/149‑1 (MQ=255)
        tGGCCGCATCATTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑Tgg                                                                                                                                          <  2:56008/149‑1 (MQ=255)
               atcatTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCAGGCGCGCC‑GGGccat                                                                                                                                      <  2:292352/146‑3 (MQ=255)
               atcatTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCAGGCGCGCC‑GGGccat                                                                                                                                      >  1:292352/1‑144 (MQ=255)
                          gTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAgg                                                                                                                        >  3:227075/1‑149 (MQ=255)
                          gTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAgg                                                                                                                        <  4:227075/149‑1 (MQ=255)
                             ccGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGt                                                                                                                      <  6:190617/148‑1 (MQ=255)
                              cgcgTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTAc                                                                                                                    >  1:261497/1‑149 (MQ=255)
                               gcgTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACg                                                                                                                   >  1:269626/1‑149 (MQ=255)
                                     aGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAg                                                                                                                                     <  1:118535/125‑1 (MQ=255)
                                     aGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAg                                                                                                                                     >  2:118535/1‑125 (MQ=255)
                                     aGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGc                                                                                                             <  1:159833/149‑1 (MQ=255)
                                              cccTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAg                                                                                                    <  6:250026/149‑1 (MQ=255)
                                                          tcCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGcc                                                                                        >  4:121057/1‑149 (MQ=255)
                                                           ccTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCg                                                                                                        <  3:69147/132‑1 (MQ=255)
                                                           ccTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCg                                                                                                        >  4:69147/1‑132 (MQ=255)
                                                                   aGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCaa                                                                               >  1:128592/1‑149 (MQ=255)
                                                                       gTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAgc                                                                           >  2:107900/1‑149 (MQ=255)
                                                                        ttGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGGGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAgcg                                                                          <  7:162662/149‑1 (MQ=255)
                                                                                gTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGacgacg                                                                  >  3:172383/1‑149 (MQ=255)
                                                                                     tGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGa                                                                                                                          >  2:284502/1‑88 (MQ=255)
                                                                                     tGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGa                                                                                                                          <  1:284502/88‑1 (MQ=255)
                                                                                     tGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGAt                                                             >  6:206021/1‑149 (MQ=255)
                                                                                           aGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATcagcag                                                       >  5:170787/1‑149 (MQ=255)
                                                                                           aGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATcagcag                                                       <  4:172383/149‑1 (MQ=255)
                                                                                              ttGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCaa                                                    <  2:128592/149‑1 (MQ=255)
                                                                                                 ggCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGc                                                                                                                             <  4:81741/73‑1 (MQ=255)
                                                                                                 ggCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGc                                                                                                                             >  3:81741/1‑73 (MQ=255)
                                                                                                  gCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCg                                                >  1:342583/1‑149 (MQ=255)
                                                                                                       tcGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCgggg                                                                                          >  4:322707/1‑102 (MQ=255)
                                                                                                       tcGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCgggg                                                                                          <  3:322707/102‑1 (MQ=255)
                                                                                                       tcGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTg                                             >  3:276754/1‑147 (MQ=255)
                                                                                                         gATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCCGTGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAg                                          >  1:81078/1‑149 (MQ=255)
                                                                                                                cgcTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTc                                  <  2:81078/149‑1 (MQ=255)
                                                                                                                cgcTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTc                                  <  2:342583/149‑1 (MQ=255)
                                                                                                                  cTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCCGGCCGCGGCCGAGGGTCCGGGCTGCCACCGGCCGGCGCTGGGGGCCCCGCACCAACCGCGCGCCGCCGCCGATCAGCGGCACCTCGCTGAAGCCGCCCTCGa                                >  2:18057/1‑149 (MQ=255)
                                                                                                                     cACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGaa                               <  5:206021/147‑1 (MQ=255)
                                                                                                                        cTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACAt                            >  3:214012/1‑147 (MQ=255)
                                                                                                                               cgcAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTgg                   <  1:18057/149‑1 (MQ=255)
                                                                                                                               cgcAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTgg                   <  2:261497/149‑1 (MQ=255)
                                                                                                                                gcAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGc                                         <  4:171917/126‑1 (MQ=255)
                                                                                                                                gcAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGc                                         >  3:171917/1‑126 (MQ=255)
                                                                                                                                gcAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTgg                   <  3:262438/148‑1 (MQ=255)
                                                                                                                                gcAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTgg                   >  4:262438/1‑148 (MQ=255)
                                                                                                                                 cAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCg                 >  3:259972/1‑149 (MQ=255)
                                                                                                                                  aaGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCgg                >  5:328068/1‑149 (MQ=255)
                                                                                                                                    ggCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCGGTc              <  4:214012/149‑1 (MQ=255)
                                                                                                                                        tGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCGGTCCGCt          <  4:259972/149‑1 (MQ=255)
                                                                                                                                             ggCCAGGCGCGCC‑‑GGGCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCggg                                                                                           >  2:262110/1‑62 (MQ=38)
                                                                                                                                             ggCCAGGCGCGCC‑‑GGGCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCggg                                                                                           <  1:262110/62‑1 (MQ=255)
                                                                                                                                               cccGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTAACTCGAACATCAGGCCTGGCGGTCCCCTTGAGAtt   >  5:305712/1‑149 (MQ=255)
                                                                                                                                                ccGGCGCGCC‑TGGCCCAGGCCGCGACCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCCCCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCGGTCCGCTTgagagct  >  1:122491/1‑146 (MQ=255)
                                                                                                                                                ccGGCGCGCC‑TGGCCCAGGCCGCGACCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCGGTCCGCTTga       <  2:122491/144‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CACGGCCGTGGCCGCATCATTGGCCGGTTCCGCGTTCAGGCTGATGCCCTGGCGGATCTCCTGTTCCAGGCGTTGCAGAGGTGCCTGGTCGAGGTTGGGCATCTCGATTTCGCGCTCCACCTGCGTGCGCAAGGCGTGCATGGCCCGGCGCGCC‑TGGCCCAGGCCGCGGCCGAGGGTACGGGCTGCCACCGGCAGGCGCTCGGGGCCCAGCACCAACAGCGCGACGACGCCGATCAGCAGCAACTCGCTGAAGCCTACCTCGAACATCAGGCCTGGCGGTCCGCTTGAGATTG  >  NC_002947/1189834‑1190126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑