Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,205,643	T→A	8.3%	Q40L (CAG→CTG)	PP_1057 ←	PadR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,205,643	0	T	A	8.3%	166.1 / 3.3	59	Q40L (CAG→CTG)	PP_1057	PadR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base A (3/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCGTTTGCTGCCTTGTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGAT  >  NC_002947/1205495‑1205787                                                                                                                                                      |                                                                                                                                                  cAGGCGTTTGCTGCCTTGTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGgct                                                                                                                                                   <  5:146419/149‑1 (MQ=255)      gTTTGCTGCCTTGTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGATGTAGCTGCCGTCGAAAAGATTTTCGATCTGGCGGATAAGTTC‑TTAACCTTGGCCGGGATGCTCg                                                                                                                                              >  2:258602/1‑149 (MQ=255)          gctgcCTTGTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGc                                                                                                                                            >  8:87037/1‑147 (MQ=255)                ttGTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGt                                                                                                                                    >  7:308378/1‑149 (MQ=255)                  gTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTc                                                                                                                                   <  3:250861/148‑1 (MQ=255)                         gCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGTCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATc                                                                                                                            <  4:65707/148‑1 (MQ=255)                              tGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGTCGGGCTGCTCGGCCAGCAAGTCCAGCATCagca‑gc                                                                                                                     >  3:57710/1‑149 (MQ=255)                              tGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCagca‑gc                                                                                                                     >  3:175187/1‑149 (MQ=255)                                    gTTCGGCCTCTTCAATGAATTTCAGCGTGGGGGATATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTCTGTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAg                                                                                                                <  1:258602/149‑1 (MQ=255)                                                cAAGGAAGTTCAGCGTGTGATAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACATATTTTCCATCTCGCGGATCAGGTC‑GTAACAGTGGCCGGTCTGCTCGGCCAGCAAGTCAAGCATCAGCA‑GCTTGAGCTCACCCgggcc                                                                                                    >  4:176555/1‑146 (MQ=255)                                                cAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTc                                                                                                                                   >  6:116537/1‑118 (MQ=255)                                                cAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTc                                                                                                                                   <  5:116537/118‑1 (MQ=255)                                                cAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATc                                                                                                                            >  4:291078/1‑125 (MQ=255)                                                cAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATc                                                                                                                            <  3:291078/125‑1 (MQ=255)                                                  aGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGTCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGaa                                                                                                 <  4:57710/149‑1 (MQ=255)                                                   ggAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCg                                                                                                   >  2:165140/1‑146 (MQ=255)                                                         tCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGCAGACAcgc                                                                                          >  1:186818/1‑149 (MQ=255)                                                                 gggTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCcgcg                                                                                  <  3:164827/149‑1 (MQ=255)                                                                       aTCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGa                                                                                                  <  8:134719/127‑1 (MQ=255)                                                                       aTCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGa                                                                                                  >  7:134719/1‑127 (MQ=255)                                                                              cAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCACGCTTGAGGTCACCCGGGGCGAAGACACGCgg                                                                                        <  1:36189/131‑1 (MQ=255)                                                                              cAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCgg                                                                                        >  2:36189/1‑130 (MQ=255)                                                                               aGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCAcgggcgg                                                                    >  5:309820/1‑149 (MQ=255)                                                                                 ggCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACgggcgggg                                                                  >  8:247249/1‑149 (MQ=255)                                                                                  gCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACgggcggggc                                                                 <  4:175187/149‑1 (MQ=255)                                                                                  gCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCACTCGCCACgggcggggc                                                                 >  1:234183/1‑149 (MQ=255)                                                                                    ttGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGgcgc                                                               >  1:89443/1‑149 (MQ=255)                                                                                        gCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCt                                                           >  8:175144/1‑149 (MQ=255)                                                                                         cTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTc                                                          >  5:22218/1‑149 (MQ=255)                                                                                          tGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTc                                                          >  2:235413/1‑148 (MQ=255)                                                                                              gCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAg                                                      <  1:235413/148‑1 (MQ=255)                                                                                              gCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAg                                                      <  8:308378/148‑1 (MQ=255)                                                                                                         aCAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAggg                                          >  6:193620/1‑149 (MQ=255)                                                                                                         aCAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAgg                                           <  4:21607/148‑1 (MQ=255)                                                                                                         aCAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTCAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCACGCCACGGGCGGGGCGCCGCTCCAAGGGAGCATGAggg                                          >  4:224234/1‑149 (MQ=255)                                                                                                           aGATTTTCGATCTGGCGGATCACGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACCCGGGCCGCGCTCGCCACGGGCGGGGCCCCGCTCGAAGGGATCATGAgggg                                         >  5:24777/1‑148 (MQ=255)                                                                                                               tttCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGt                                    >  7:58639/1‑149 (MQ=255)                                                                                                                        ggCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCa                             >  3:139376/1‑147 (MQ=255)                                                                                                                         gCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCcgcg                                                                                  <  4:35171/93‑1 (MQ=255)                                                                                                                         gCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCcgcg                                                                                  >  3:35171/1‑93 (MQ=255)                                                                                                                         gCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCACTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTcgcg                               <  2:234183/144‑1 (MQ=255)                                                                                                                          cGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCatagg                         <  7:175144/149‑1 (MQ=255)                                                                                                                              tCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCatagggata                     <  7:247249/149‑1 (MQ=255)                                                                                                                                aGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCCCgcccgtggcgagc                                                             <  2:14276/107‑4 (MQ=255)                                                                                                                                aGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCatagggatag                    <  1:165140/148‑1 (MQ=255)                                                                                                                                 ggTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCatagggataggg                  <  3:176555/149‑1 (MQ=255)                                                                                                                                           gctGGCCGAGCAGCCCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAgggg                                         <  2:57526/115‑1 (MQ=255)                                                                                                                                           gctGGCCGAGCAGCCCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAgggg                                         >  1:57526/3‑117 (MQ=255)                                                                                                                                           cGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCcgtcgt         >  4:307268/1‑149 (MQ=255)                                                                                                                                            gTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAgg                                                                                                               <  2:169279/46‑1 (MQ=255)                                                                                                                                             tGGCCGAGCAGCCCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGaa                                                       >  7:49808/1‑101 (MQ=255)                                                                                                                                             tGGCCGAGCAGCCCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGaa                                                       <  8:49808/101‑1 (MQ=255)                                                                                                                                             tGGCCGAGCAGCCCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGttt       >  6:58476/1‑149 (MQ=255)                                                                                                                                               gCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGGCACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTg      <  6:304186/148‑1 (MQ=255)                                                                                                                                               gCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGc     <  5:193620/149‑1 (MQ=255)                                                                                                                                               gCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGc     >  4:83036/1‑149 (MQ=255)                                                                                                                                                ccGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGGCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCg    <  6:22218/149‑1 (MQ=255)                                                                                                                                                 cGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGa   <  4:139376/149‑1 (MQ=255)                                                                                                                                                 cGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGa   <  8:58639/149‑1 (MQ=255)                                                                                                                                                  gggCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGat  <  2:89443/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CAGGCGTTTGCTGCCTTGTACCTGGCCGCTGATCAGTTCGGCCTCTTCAAGGAAGTTCAGCGTGGGGTAGATCACTCCAGGGCTTGGGCTGTAGCTGCCGTCGAACAGATTTTCGATCTGGCGGATCAGGTC‑GTAACCGTGGCCGGGCTGCTCGGCCAGCAAGTCCAGCATCAGCA‑GCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGAT  >  NC_002947/1205495‑1205787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑