| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,210,829 | T→A | 18.9% | intergenic (+19/+18) | ytnA → / ← PP_1060 | amino acid permease YtnA/glutamate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,210,829 | 0 | T | A | 18.9% | 59.8 / 8.1 | 31 | intergenic (+19/+18) | ytnA/PP_1060 | amino acid permease YtnA/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/17); new base A (4/2); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01 | |||||||||||
GGCGTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGCGA > NC_002947/1210681‑1210975 | ggCGTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCgtt > 2:90805/1‑149 (MQ=255) tACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCgg < 6:146631/149‑1 (MQ=255) cTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGcc < 1:309728/149‑1 (MQ=255) ttCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTAGTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGCGGTTCGGccc > 2:296769/1‑149 (MQ=255) gAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGCCCCac < 5:21918/149‑2 (MQ=255) aTACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGtt < 1:296769/149‑1 (MQ=255) cgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGc > 4:185638/1‑132 (MQ=255) cgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGc < 3:185638/132‑1 (MQ=255) gCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGgccg < 3:196999/149‑1 (MQ=255) ccTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGcccc < 7:60069/130‑1 (MQ=255) ccTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGcccc > 8:60069/1‑130 (MQ=255) tACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTAGAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACt < 4:46904/148‑1 (MQ=255) gCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCtgtg < 5:350677/149‑1 (MQ=255) cTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGaaa < 7:91758/148‑1 (MQ=255) aaGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGtt < 7:130777/149‑1 (MQ=255) gACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCACTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCa < 2:198010/149‑1 (MQ=255) cGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCAcc < 5:161321/149‑1 (MQ=255) aCGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGTACTGGCCGTTCACCTCGCCGGTcagca < 1:88432/149‑1 (MQ=255) aCAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGGTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGTATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCa < 3:300679/148‑1 (MQ=255) cctgacctgaCGTGGGGCCGCGTTTCGGCCCCACCGTTCTATAAGGCCCCCACATAGCTGTGCGGCGCGAAACAGTCCGCCCCGGCCCGTTGCCACATCCCCGAATAGAACAGGCCGATCAACAAGCCGGTCAGCAGCACACCAGGca > 4:213694/1‑147 (MQ=255) acctgacGTGGGGCCGCTTTGCGTCCACAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCTCCCGGGCCAGTTGCCATATCCTCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCaa < 3:266144/148‑1 (MQ=255) ctgacGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGaa < 8:206924/149‑1 (MQ=255) aacgctGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTc > 6:171250/6‑133 (MQ=255) gctGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAAcaca > 4:183029/3‑149 (MQ=255) gTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCtt > 4:201207/1‑138 (MQ=255) gTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCtt < 3:201207/138‑1 (MQ=255) gTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt < 3:213694/149‑1 (MQ=255) ctGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt > 3:124439/2‑149 (MQ=255) gCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATc > 7:85219/1‑149 (MQ=255) gCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATc > 8:61026/1‑149 (MQ=255) gCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCt > 5:119127/1‑147 (MQ=255) gCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACATCGCCGGTCAGCAGCACACCTGGCTTCAAGAACACATGCATCTGc > 4:86077/1‑149 (MQ=255) tttGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCtt > 4:324832/1‑128 (MQ=255) tttGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCtt < 3:324832/128‑1 (MQ=255) ttGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGCga < 6:312848/148‑1 (MQ=255) | GGCGTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGCGA > NC_002947/1210681‑1210975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |