Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,232,736	G→C	11.0%	intergenic (‑24/+193)	glpD ← / ← glpR	aerobic glycerol‑3‑phosphate dehydrogenase/DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,232,736	0	G	C	11.0%	151.7 / 10.1	56	intergenic (‑24/+193)	glpD/glpR	aerobic glycerol‑3‑phosphate dehydrogenase/DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base G (25/23);  new base C (4/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

CTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGC  >  NC_002947/1232590‑1232879                                                                                                                                                    |                                                                                                                                                cTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGaa                                                                                                                                               >  2:36659/1‑149 (MQ=255)     aaGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGGAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCCAAGGCCTCCTGAGGCTGTTCACATCGAAtgt                                                                                                                                            <  1:36659/149‑1 (MQ=255)     aaGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAAtgt                                                                                                                                            >  5:71626/1‑148 (MQ=255)          gcgACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATTCGATGTGAAc                                                                                                                                        >  8:42793/1‑147 (MQ=255)          gcgACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATTCGATGTGAAc                                                                                                                                        <  7:42793/147‑1 (MQ=255)           cgACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCAAATGTGAACAtt                                                                                                                                     <  8:280272/149‑1 (MQ=255)           cgACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCACAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACAtt                                                                                                                                     <  7:90821/149‑1 (MQ=255)            gACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTg                                                                                                                                    <  5:312437/149‑1 (MQ=255)                 gcggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGtt                                                                                                                               >  8:53742/1‑149 (MQ=255)                 atggcgTGGGCATCGCTGCCGACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGtt                                                                                                                               >  6:167122/3‑149 (MQ=255)                     cgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGtt                                                                                                                           <  7:111028/149‑1 (MQ=255)                       tCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTg                                                                                                                         >  7:131784/1‑149 (MQ=255)                                   cccACTCCTTTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTTAGGCTGTTCACATCTAATGTGAACATTGATGTTCGTTTGCGAAAATATTa                                                                                                              <  1:125577/148‑1 (MQ=255)                                   cccACGCCATTGATGCCACCTCCGATTACGGCTAGGTCATAGGAGTTGGCTGTTGGGGGCTGGGACTAAACTGGCTGTGACTCGGG‑CAAGGCCTCCTGAGGCTGTTGAGATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTa                                                                                                              <  5:102020/148‑1 (MQ=255)                                   cccACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTa                                                                                                              <  7:53742/148‑1 (MQ=255)                                    ccACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGc                                                                                                                        <  6:72454/137‑1 (MQ=255)                                    ccACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGc                                                                                                                        >  5:72454/1‑137 (MQ=255)                                    ccACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAgc                                                                                                            <  2:56241/149‑1 (MQ=255)                                     cACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTTAGGCTGTTCACATCGAATGTGAACATTGAt                                                                                                                                  <  8:272181/126‑1 (MQ=255)                                     cACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCATGAGGCTGTTCACATCGAATGTGAACATTGAt                                                                                                                                  >  7:272181/1‑126 (MQ=255)                                        gCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCa                                                                                                         >  6:116341/1‑148 (MQ=255)                                               aTGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACg                                                                                                 >  3:25523/1‑149 (MQ=255)                                                   cACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCgg                                                                                             >  3:199194/1‑149 (MQ=255)                                                    aCCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGt                                                                                            <  6:71626/149‑1 (MQ=255)                                                               cGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCcagtcag                                                                                  <  2:47318/148‑1 (MQ=255)                                                                     ggTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtat                                                                           <  5:167122/149‑1 (MQ=255)                                                                     ggTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtat                                                                           <  4:221107/149‑1 (MQ=255)                                                                       tCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCg                                                                                          <  4:114466/132‑1 (MQ=255)                                                                       tCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCg                                                                                          >  3:114466/1‑132 (MQ=255)                                                                                     tGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCa                                                            >  5:329394/1‑148 (MQ=255)                                                                                         gggggCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAAt                                                         >  3:161561/1‑147 (MQ=255)                                                                                         gggggCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAAt                                                         <  4:161561/147‑1 (MQ=255)                                                                                             gCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAAt                                                   >  5:291493/1‑149 (MQ=255)                                                                                             gCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCATGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAAt                                                   <  4:199194/149‑1 (MQ=255)                                                                                               tGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGAGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATg                                                  >  4:4191/1‑148 (MQ=255)                                                                                                   aCGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCAAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGt                                              >  7:271583/1‑148 (MQ=255)                                                                                                      aaaCTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGaa                                           >  6:263215/1‑148 (MQ=255)                                                                                                              tggGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGc                                                                                                >  8:13021/1‑87 (MQ=255)                                                                                                              tggGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGc                                                                                                <  7:13021/87‑1 (MQ=255)                                                                                                                    acGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCaaa                                                          >  2:138929/1‑119 (MQ=255)                                                                                                                       gg‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAgg                         <  5:116341/149‑1 (MQ=255)                                                                                                                       gg‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAgg                         <  6:329394/149‑1 (MQ=255)                                                                                                                             ggCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACg                                   >  3:207769/1‑134 (MQ=255)                                                                                                                             ggCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACg                                   <  4:207769/134‑1 (MQ=255)                                                                                                                                ctcctGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACgg                 >  8:242247/1‑149 (MQ=255)                                                                                                                                   ctGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCtt              <  5:263215/149‑1 (MQ=255)                                                                                                                                     atcaatGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGaaa                                <  2:320453/124‑1 (MQ=255)                                                                                                                                     atcaatGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGaaa                                >  1:320453/6‑129 (MQ=255)                                                                                                                                      aGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTgg            <  7:180914/148‑1 (MQ=255)                                                                                                                                      aGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTgg            >  8:180914/1‑148 (MQ=255)                                                                                                                                         atGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGt          >  5:280554/2‑147 (MQ=255)                                                                                                                                          tGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAg       >  3:147462/1‑149 (MQ=255)                                                                                                                                           gTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAgg      >  5:118651/1‑149 (MQ=255)                                                                                                                                           gTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAgg      >  2:126131/1‑149 (MQ=255)                                                                                                                                            ttCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAggg     <  8:131784/149‑1 (MQ=255)                                                                                                                                               acaTCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgc  >  6:222632/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                CTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGG‑CAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGC  >  NC_002947/1232590‑1232879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑