| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,233,769 | G→T | 11.8% | intergenic (‑85/+17) | glpR ← / ← glpK | DNA‑binding transcriptional repressor GlpR/glycerol kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,233,769 | 0 | G | T | 11.8% | 143.4 / 9.9 | 51 | intergenic (‑85/+17) | glpR/glpK | DNA‑binding transcriptional repressor GlpR/glycerol kinase |
| Reads supporting (aligned to +/- strand): ref base G (23/22); new base T (2/4); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
ACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTCGCGCAGCT > NC_002947/1233626‑1233916 | acacAGCCGCCTTCGCGAACCTCTACTCGCATTTGTCCTTCCCGGGGGCCCCGACTCATGCCCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATCCCGTAGGAAGGGGATC‑ACGCCCAGTAGACGATTCGGGCCGCATggcg < 8:209722/147‑1 (MQ=255) cATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCCATGCGGcc < 6:2417/149‑1 (MQ=255) aGCCGGGTTCGCGTACCAGTTCGCGGATTGGTTGTGGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCGGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGTTTGGGGCCGCCATGCGGCCCCa < 7:216212/149‑1 (MQ=255) gCCCCGTCCGCGTACCAGGTCGAGGATTTGTTGTTGGCGGGGCGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCCATGCGGCCCCaa < 3:142152/149‑1 (MQ=255) ccGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATGAACGGCCAGTAGACGATTGGGGCCGCCATGCGGCCCCaa < 1:61349/149‑1 (MQ=255) ccGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCaa > 6:200781/1‑148 (MQ=255) cgcgTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACa < 5:200781/149‑1 (MQ=255) gTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGc > 6:297677/1‑148 (MQ=255) gcgTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCa > 1:301239/1‑149 (MQ=255) tACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGc < 2:301239/149‑1 (MQ=255) tACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGc < 6:229270/149‑1 (MQ=255) gTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCCATGCGGCCCCaatc > 3:212281/1‑132 (MQ=255) tCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGa > 8:251928/1‑149 (MQ=255) aGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCt < 6:58611/149‑1 (MQ=255) ttgGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACg > 6:215518/1‑149 (MQ=255) cAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGAc < 7:251928/149‑1 (MQ=255) aTTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGTCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTAt < 2:45778/108‑1 (MQ=255) aTTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGTCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTAt > 1:45778/1‑108 (MQ=255) aTTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGc > 3:129583/1‑149 (MQ=255) cATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCct < 5:311443/148‑1 (MQ=255) cATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCct < 5:218495/148‑1 (MQ=255) cGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACTGCCttcttc > 8:180891/1‑147 (MQ=255) tCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTccc < 7:291697/115‑1 (MQ=255) tCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTccc > 8:291697/1‑115 (MQ=255) tCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGcc > 2:307635/1‑149 (MQ=255) tttGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCgg > 7:116416/1‑148 (MQ=255) cGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCtttt < 1:307635/149‑1 (MQ=255) ggACAAATTCTGCAATGATGCCGTAGGAATGGGATT‑ACGGACAGAAGACGATTGGGGCCGCAAGGCGGCCCCAACAGCTATCATGCCTAGTGATCTTCCCAGACACGTGTACGATCGAACTCATTCTTCCACCCGGAATACAGAtttac > 7:60632/1‑147 (MQ=255) ggACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTtttc > 2:174489/1‑149 (MQ=255) gACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTc > 8:94169/1‑88 (MQ=255) gACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTc < 7:94169/88‑1 (MQ=255) ttCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGc < 5:297677/149‑1 (MQ=255) cTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGt > 2:228033/1‑149 (MQ=255) tGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGtt < 1:174489/149‑1 (MQ=255) aaTGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCttt > 6:315733/1‑134 (MQ=255) aaTGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCttt < 5:315733/134‑1 (MQ=255) cGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAg < 7:126269/81‑1 (MQ=255) cGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAg > 8:126269/1‑81 (MQ=255) cGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTg > 4:175130/1‑149 (MQ=255) aaGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCt > 7:315925/1‑149 (MQ=255) aGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTg < 8:116416/149‑1 (MQ=255) ggATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAAc > 5:253821/1‑149 (MQ=255) aTG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTc < 3:175130/149‑1 (MQ=255) g‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCTGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCAc > 1:280991/1‑149 (MQ=255) cGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGc < 4:129583/149‑1 (MQ=255) gCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTc < 1:228033/149‑1 (MQ=255) aGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCgat < 6:253821/149‑1 (MQ=255) tAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCgatga > 2:132397/1‑149 (MQ=255) ggggCCGCCATGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGt > 2:29249/1‑149 (MQ=255) gggCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTc < 8:315925/149‑1 (MQ=255) gCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTcgc > 5:354567/1‑149 (MQ=255) tGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGCTGATCGCCTTGTCGCGCAGCt < 6:354567/149‑1 (MQ=255) | ACATAGCCGCGTTCGCGTACCAGTTCGAGGATTTGTTGTTGGCGGGGGGGCAGATTCATGGGCGGTCCTTTGGGGCTGCCGGACAAATTCTGCAATGATGCCGTAGGAAGGGGATG‑ACGGCCAGTAGACGATTGGGGCCGCATGGCGGCCCCAACAGCTATCATGCCTCGTGATCTTCCCAGTCACGGGTACGCTCGACCGCCTTCTTCCAGCCGGCATACAGCTTTTCTTTCTGCGTTTCGTCCAGTTGCGGGCTGAACTCACGCTCGATGATCGCCTTGTCGCGCAGCT > NC_002947/1233626‑1233916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |