Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,245,701 T→G 7.1% L19R (CTG→CGG)  PP_1087 → OmpA family outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,245,7010TG 7.1% 171.8 / 4.0 56L19R (CTG→CGG) PP_1087OmpA family outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTT  >  NC_002947/1245558‑1245848
                                                                                                                                               |                                                                                                                                                    
ccAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccgg                                                                                                                                                 >  8:280077/1‑149 (MQ=255)
  aaTCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggcc                                                                                                                                               >  7:123254/1‑149 (MQ=255)
         ccGGAAAACGCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCGGCCGGCAGGCTGGCGAAc                                                                                                                                        <  2:251023/149‑1 (MQ=255)
          cGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACt                                                                                                                                       >  2:58238/1‑149 (MQ=255)
                 ccccGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                <  7:280077/149‑1 (MQ=255)
                  cccGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTcc                                                                                                                               <  6:177966/149‑1 (MQ=255)
                    cGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                >  3:284157/1‑146 (MQ=255)
                    cGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                >  8:196041/1‑146 (MQ=255)
                        gCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGAcgc                                                                                                                         <  8:123254/149‑1 (MQ=255)
                          gAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGAcgcg                                                                                                                        >  1:340482/1‑148 (MQ=255)
                                ggCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGgag                                                                                                                 <  4:284157/149‑1 (MQ=255)
                                      cAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCCGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACAtt                                                                                                           >  5:4079/1‑149 (MQ=255)
                                       aaGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTg                                                                                                          <  2:340482/149‑1 (MQ=255)
                                        aGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGc                                                                                                         >  8:154283/1‑149 (MQ=255)
                                             tCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTgg                                                                                                     <  5:71622/148‑1 (MQ=255)
                                              ccGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTgg                                                                                                     >  4:188074/1‑147 (MQ=255)
                                               cGGACAGCGTCCGATAGCCTGCTGGTAAGCCGGCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGaa                                                                                                   <  7:323079/148‑1 (MQ=255)
                                                ggACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAgg                                                                                                 >  2:45560/1‑149 (MQ=255)
                                                    aGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTc                                                                                               >  4:52689/1‑147 (MQ=255)
                                                      cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                          <  7:18739/149‑1 (MQ=255)
                                                      cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                          <  4:158814/149‑1 (MQ=255)
                                                           gATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGAc                                                                                     <  6:4079/149‑1 (MQ=255)
                                                            aTAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGAc                                                                                     >  1:232598/1‑148 (MQ=255)
                                                             tAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCa                                                                                   <  3:52689/149‑1 (MQ=255)
                                                                    ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                          >  3:52111/1‑135 (MQ=255)
                                                                    ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                          <  4:52111/135‑1 (MQ=255)
                                                                    ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAg                                                                            <  7:154283/149‑1 (MQ=255)
                                                                       gTAAGCCGTCAGATTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAgtgt                                                                         >  1:304893/1‑149 (MQ=255)
                                                                       gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAgtg                                                                          <  2:232598/148‑1 (MQ=255)
                                                                            ccGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCt                                                                    >  8:73830/1‑149 (MQ=255)
                                                                            ccGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCt                                                                    <  3:188074/149‑1 (MQ=255)
                                                                              gTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGa                                                                  >  8:287360/1‑149 (MQ=255)
                                                                               tCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGGAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGag                                                                 >  8:202899/1‑149 (MQ=255)
                                                                                   aaTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGagccag                                                             >  4:30544/1‑149 (MQ=255)
                                                                                          ggAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATc                                                       >  1:7910/1‑148 (MQ=255)
                                                                                           gAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGa                                                     <  2:247285/149‑1 (MQ=255)
                                                                                            aGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGaa                                                    <  1:58238/149‑1 (MQ=255)
                                                                                                gccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGtt                                                >  4:310034/1‑149 (MQ=255)
                                                                                                 cccGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGttt                                               <  7:196041/149‑1 (MQ=255)
                                                                                                  ccGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGGAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTc                                              <  7:202899/149‑1 (MQ=255)
                                                                                                     tGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCgg                                            >  2:67656/1‑148 (MQ=255)
                                                                                                          cAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTTCCTGGAAGGTCGCAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                       <  1:90208/149‑1 (MQ=255)
                                                                                                           aGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTgtc                                                                        >  2:95909/1‑114 (MQ=255)
                                                                                                                  ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTc                               <  7:287360/148‑1 (MQ=255)
                                                                                                                  ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCg                              >  6:70256/1‑149 (MQ=255)
                                                                                                                   cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt                             >  3:327385/1‑149 (MQ=255)
                                                                                                                        ccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTc                        <  3:113603/149‑1 (MQ=255)
                                                                                                                            ttGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCgg                      <  4:327385/147‑1 (MQ=255)
                                                                                                                              gACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAg                  >  1:339079/1‑149 (MQ=255)
                                                                                                                                 ccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc                >  5:95913/1‑148 (MQ=255)
                                                                                                                                   agtgcGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGag             >  1:200769/5‑149 (MQ=255)
                                                                                                                                       cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                       <  6:259511/119‑1 (MQ=255)
                                                                                                                                       cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                       >  5:259511/1‑119 (MQ=255)
                                                                                                                                        gccggccgGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGaa                                                    >  3:36471/1‑105 (MQ=255)
                                                                                                                                        gccggccgGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGaa                                                    <  4:36471/105‑1 (MQ=255)
                                                                                                                                          cAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCTGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCgg      <  1:45560/149‑1 (MQ=255)
                                                                                                                                             ccTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGATCACATTGCCGGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGgtgt   <  7:216069/149‑1 (MQ=255)
                                                                                                                                              cTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGtt  <  7:73830/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTT  >  NC_002947/1245558‑1245848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑