Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,245,708	C→T	10.6%	A21A (GCC→GCT)	PP_1087 →	OmpA family outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,245,708	0	C	T	10.6%	151.7 / 6.1	56	A21A (GCC→GCT)	PP_1087	OmpA family outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base T (3/3);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

AATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTCA  >  NC_002947/1245560‑1245851                                                                                                                                                     |                                                                                                                                                 aaTCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggcc                                                                                                                                                  >  7:123254/1‑149 (MQ=255)        ccGGAAAACGCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCGGCCGGCAGGCTGGCGAAc                                                                                                                                           <  2:251023/149‑1 (MQ=255)         cGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACt                                                                                                                                          >  2:58238/1‑149 (MQ=255)                ccccGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                   <  7:280077/149‑1 (MQ=255)                 cccGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTcc                                                                                                                                  <  6:177966/149‑1 (MQ=255)                   cGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                   >  3:284157/1‑146 (MQ=255)                   cGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                   >  8:196041/1‑146 (MQ=255)                       gCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGAcgc                                                                                                                            <  8:123254/149‑1 (MQ=255)                         gAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGAcgcg                                                                                                                           >  1:340482/1‑148 (MQ=255)                               ggCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGgag                                                                                                                    <  4:284157/149‑1 (MQ=255)                                     cAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCCGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACAtt                                                                                                              >  5:4079/1‑149 (MQ=255)                                      aaGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTg                                                                                                             <  2:340482/149‑1 (MQ=255)                                       aGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGc                                                                                                            >  8:154283/1‑149 (MQ=255)                                            tCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTgg                                                                                                        <  5:71622/148‑1 (MQ=255)                                             ccGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTgg                                                                                                        >  4:188074/1‑147 (MQ=255)                                              cGGACAGCGTCCGATAGCCTGCTGGTAAGCCGGCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGaa                                                                                                      <  7:323079/148‑1 (MQ=255)                                               ggACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAgg                                                                                                    >  2:45560/1‑149 (MQ=255)                                                   aGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTc                                                                                                  >  4:52689/1‑147 (MQ=255)                                                     cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                             <  4:158814/149‑1 (MQ=255)                                                     cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                             <  7:18739/149‑1 (MQ=255)                                                          gATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGAc                                                                                        <  6:4079/149‑1 (MQ=255)                                                           aTAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGAc                                                                                        >  1:232598/1‑148 (MQ=255)                                                            tAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCa                                                                                      <  3:52689/149‑1 (MQ=255)                                                                   ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                             >  3:52111/1‑135 (MQ=255)                                                                   ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑Agg                                                                                             <  4:52111/135‑1 (MQ=255)                                                                   ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAg                                                                               <  7:154283/149‑1 (MQ=255)                                                                      gTAAGCCGTCAGATTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAgtgt                                                                            >  1:304893/1‑149 (MQ=255)                                                                      gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAgtg                                                                             <  2:232598/148‑1 (MQ=255)                                                                           ccGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCt                                                                       >  8:73830/1‑149 (MQ=255)                                                                           ccGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCt                                                                       <  3:188074/149‑1 (MQ=255)                                                                             gTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGa                                                                     >  8:287360/1‑149 (MQ=255)                                                                              tCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGGAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGag                                                                    >  8:202899/1‑149 (MQ=255)                                                                                  aaTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGagccag                                                                >  4:30544/1‑149 (MQ=255)                                                                                         ggAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATc                                                          >  1:7910/1‑148 (MQ=255)                                                                                          gAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGa                                                        <  2:247285/149‑1 (MQ=255)                                                                                           aGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGaa                                                       <  1:58238/149‑1 (MQ=255)                                                                                               gccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGtt                                                   >  4:310034/1‑149 (MQ=255)                                                                                                cccGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGttt                                                  <  7:196041/149‑1 (MQ=255)                                                                                                 ccGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGGAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTc                                                 <  7:202899/149‑1 (MQ=255)                                                                                                    tGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCgg                                               >  2:67656/1‑148 (MQ=255)                                                                                                         cAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTTCCTGGAAGGTCGCAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                          <  1:90208/149‑1 (MQ=255)                                                                                                          aGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTgtc                                                                           >  2:95909/1‑114 (MQ=255)                                                                                                                 ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTc                                  <  7:287360/148‑1 (MQ=255)                                                                                                                 ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCg                                 >  6:70256/1‑149 (MQ=255)                                                                                                                  cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt                                >  3:327385/1‑149 (MQ=255)                                                                                                                       ccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTc                           <  3:113603/149‑1 (MQ=255)                                                                                                                           ttGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCgg                         <  4:327385/147‑1 (MQ=255)                                                                                                                             gACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAg                     >  1:339079/1‑149 (MQ=255)                                                                                                                                ccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc                   >  5:95913/1‑148 (MQ=255)                                                                                                                                  agtgcGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGag                >  1:200769/5‑149 (MQ=255)                                                                                                                                      cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                          <  6:259511/119‑1 (MQ=255)                                                                                                                                      cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                          >  5:259511/1‑119 (MQ=255)                                                                                                                                       gccggccgGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGaa                                                       >  3:36471/1‑105 (MQ=255)                                                                                                                                       gccggccgGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGaa                                                       <  4:36471/105‑1 (MQ=255)                                                                                                                                         cAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCTGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCgg         <  1:45560/149‑1 (MQ=255)                                                                                                                                            ccTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGATCACATTGCCGGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGgtgt      <  7:216069/149‑1 (MQ=255)                                                                                                                                             cTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGtt     <  7:73830/149‑1 (MQ=255)                                                                                                                                                ccggccggCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTCa  <  5:70256/149‑1 (MQ=255)                                                                                                                                                ccggccggCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTCa  <  1:67656/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 AATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCG‑AGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTCA  >  NC_002947/1245560‑1245851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑