Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,266,910 G→C 5.6% R360G (CGC→GGC)  quiP ← acyl‑homoserine lactone acylase QuiP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,266,9100GC 5.6% 263.9 / 3.1 83R360G (CGC→GGC) quiPacyl‑homoserine lactone acylase QuiP
Reads supporting (aligned to +/- strand):  ref base G (42/36);  new base C (2/3);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATT  >  NC_002947/1266764‑1267058
                                                                                                                                                     |                                                                                                                                                    
cAGGCTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTgcgg                                                                                                                                                     <  3:148543/148‑1 (MQ=255)
 aGGCTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTgcgggc                                                                                                                                                   <  5:88569/149‑1 (MQ=255)
     tGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTgcgggcgcgg                                                                                                                                               <  8:155294/149‑1 (MQ=255)
        gATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGcc                                                                                                                                            >  8:151544/1‑149 (MQ=255)
            aCCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCa                                                                                                                                        <  1:222041/149‑1 (MQ=255)
              cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATGACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCTGCAGc                                                                                                                                      >  3:260374/1‑149 (MQ=255)
              cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGc                                                                                                                                      >  4:49784/1‑149 (MQ=255)
              cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGc                                                                                                                                      >  2:196998/1‑149 (MQ=255)
              cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCCGGCCCGCGCCCGCAGc                                                                                                                                      <  4:199952/149‑1 (MQ=255)
              cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCCGGCCCGCGCCCGCAGc                                                                                                                                      <  3:165107/149‑1 (MQ=255)
               tttGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGc                                                                                                                                      >  1:137616/1‑148 (MQ=255)
               tttGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGc                                                                                                                                      >  2:216256/1‑148 (MQ=255)
                  gAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCAt                                                                                                                                   <  1:252841/148‑1 (MQ=255)
                   aaCTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCAtt                                                                                                                                  >  5:288215/1‑148 (MQ=255)
                    aCTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGTTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTa                                                                                                                                <  1:216256/149‑1 (MQ=255)
                    aCTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTa                                                                                                                                >  7:31647/1‑149 (MQ=255)
                          gCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCg                                                                                                                          >  5:347787/1‑149 (MQ=255)
                          gCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCg                                                                                                                          <  6:69206/149‑1 (MQ=255)
                            aGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGc                                                                                                                        >  1:339417/1‑149 (MQ=255)
                               ttCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTACTGGTGAGAGGTTTCGGTGCGGGCGCGGGCCGGCGGCCATTAACAATCGGcc                                                                                                                       >  1:287495/1‑149 (MQ=255)
                               ttCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCCGGCCCGCGCCCGCAGcgaaa                                                                                                                                  <  4:257490/136‑5 (MQ=255)
                               ttCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCCGGCCCGCGCCCGCAGcgaaa                                                                                                                                  >  3:257490/1‑132 (MQ=255)
                                  aGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGt                                                                                                                   <  2:275948/148‑1 (MQ=255)
                                  aGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTa                                                                                                                  <  3:49784/149‑1 (MQ=255)
                                      ccaggcccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCt                                                                                                               >  2:227574/1‑148 (MQ=255)
                                            ccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGa                                                                                                        <  7:151544/149‑1 (MQ=255)
                                            ccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGa                                                                                                        >  5:154676/1‑149 (MQ=255)
                                             caggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGAc                                                                                                       >  8:246014/1‑149 (MQ=255)
                                                gcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGc                                                                                                    <  1:312233/149‑1 (MQ=255)
                                                  tGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCt                                                                                                  <  2:339417/149‑1 (MQ=255)
                                                  tGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCt                                                                                                  >  4:63931/1‑149 (MQ=255)
                                                    gCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCt                                                                                                  <  1:227574/147‑1 (MQ=255)
                                                     cGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTgg                                                                                               <  6:288215/149‑1 (MQ=255)
                                                     cGTTTTCTGGCGGCGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTgg                                                                                               <  8:300519/149‑1 (MQ=255)
                                                      gTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTggc                                                                                              >  2:702/1‑149 (MQ=255)
                                                            tggctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGgcggcg                                                                                          >  4:99986/1‑147 (MQ=255)
                                                               ctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAgct                                                                                     <  7:246014/149‑1 (MQ=255)
                                                                tggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCTCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGAGGCTCAgct                                                                                     >  1:90546/1‑149 (MQ=255)
                                                                    ccAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTc                                                                                 >  1:136043/1‑148 (MQ=255)
                                                                     cagcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTc                                                                                 >  8:170787/1‑147 (MQ=255)
                                                                     cagcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGa                                                                               <  2:132965/149‑1 (MQ=255)
                                                                      agcagGGTGCCGTGGCGGGTGTTGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAg                                                                              >  5:85132/1‑149 (MQ=255)
                                                                      agcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCa                                                                                                                      <  7:253113/109‑1 (MQ=255)
                                                                      agcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCa                                                                                                                      >  8:253113/1‑109 (MQ=255)
                                                                       gcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGCCGCAGCTGCTCGAgg                                                                             <  6:347787/149‑1 (MQ=255)
                                                                       gcagGCTGCCGTGGCGCGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAgg                                                                             <  4:260374/149‑1 (MQ=255)
                                                                         agGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGaa                                                                           <  4:64314/149‑1 (MQ=255)
                                                                          gggTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCg                                                                                                                          >  6:144461/1‑101 (MQ=255)
                                                                          gggTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCg                                                                                                                          <  5:144461/101‑1 (MQ=255)
                                                                               ccGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTAGGCGGCGCAGCTGCTCGAGGAACAGGTc                                                                     <  1:196998/149‑1 (MQ=255)
                                                                                   ggCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                 <  2:90546/149‑1 (MQ=255)
                                                                                    gCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGAt                                                                >  2:176867/1‑149 (MQ=255)
                                                                                       ggTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGt                                                             >  6:231620/1‑149 (MQ=255)
                                                                                         tgtCGTACATCACTTCGGGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCGTTGGCGGCGCAGCTGCTCGATGAACAGGTCCTGATTGTCg                                                           <  7:193769/149‑1 (MQ=255)
                                                                                           tCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGc                                                         <  6:154676/149‑1 (MQ=255)
                                                                                            cGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGc                                                         >  5:61840/1‑148 (MQ=255)
                                                                                             gTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCa                                                       >  5:344332/1‑149 (MQ=255)
                                                                                             gTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCa                                                       >  5:254196/1‑149 (MQ=255)
                                                                                              tACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCAt                                                      <  6:254196/149‑1 (MQ=255)
                                                                                                cATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGAtt                                                               >  7:195444/1‑138 (MQ=255)
                                                                                                cATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGAtt                                                               <  8:195444/138‑1 (MQ=255)
                                                                                                 aTCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGAc                                                   >  2:276836/1‑149 (MQ=255)
                                                                                                      ttCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCgcgc                                              >  7:14824/1‑149 (MQ=255)
                                                                                                           gcACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgctgctg                                         <  3:63931/149‑1 (MQ=255)
                                                                                                           gcACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgctgctg                                         <  8:14824/149‑1 (MQ=255)
                                                                                                              cGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTc                                      <  1:702/149‑1 (MQ=255)
                                                                                                                 ggCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCgcgc                                              <  7:174410/138‑1 (MQ=255)
                                                                                                                 ggCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCgcgc                                              >  8:174410/1‑138 (MQ=255)
                                                                                                                    g‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAgg                                                                       >  7:3116/1‑110 (MQ=255)
                                                                                                                    g‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAgg                                                                       <  8:3116/110‑1 (MQ=255)
                                                                                                                      ttGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCa                               <  3:99986/149‑1 (MQ=255)
                                                                                                                        ggCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAg                              <  6:85132/148‑1 (MQ=255)
                                                                                                                          ccACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCtt                           >  7:264115/1‑149 (MQ=255)
                                                                                                                              gTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgctgctg                                         >  7:231386/1‑131 (MQ=255)
                                                                                                                              gTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgctgctg                                         <  8:231386/131‑1 (MQ=255)
                                                                                                                              gTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCa                       >  3:51195/1‑149 (MQ=255)
                                                                                                                               tAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCAt                      >  3:311231/1‑149 (MQ=255)
                                                                                                                                aC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCAtt                     >  4:319646/1‑149 (MQ=255)
                                                                                                                                     aG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAg                 >  8:7851/1‑149 (MQ=255)
                                                                                                                                     aG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTACTGCTCCAGGCCAGCTTGCCATTGTAg                 >  1:165996/1‑149 (MQ=255)
                                                                                                                                          ggTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg              <  7:7851/148‑1 (MQ=255)
                                                                                                                                          ggTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg              <  5:231620/148‑1 (MQ=255)
                                                                                                                                          ggTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg              <  8:264115/148‑1 (MQ=255)
                                                                                                                                            tttCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCTCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGc            >  6:243911/1‑148 (MQ=255)
                                                                                                                                             ttCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGc            >  8:23007/1‑147 (MQ=255)
                                                                                                                                              tCGCTGCCGGCGCGGGCCGGCAGCCATTAACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCAAGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAg         >  8:31942/1‑149 (MQ=255)
                                                                                                                                                   gcgggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTCCCATTGTAGCCGGCCAGCACaa    >  4:141288/1‑149 (MQ=255)
                                                                                                                                                    cgggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAAt   <  2:274920/149‑1 (MQ=255)
                                                                                                                                                     gggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAAtt  <  6:344332/149‑1 (MQ=255)
                                                                                                                                                     gggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTACTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAAtt  <  2:165996/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGGCTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCG‑TTGGCCACGTAC‑AAAG‑TAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAGCTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATT  >  NC_002947/1266764‑1267058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑