Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,278,483	A→T	6.0%	intergenic (‑310/+253)	PP_5465 ← / ← PP_1117	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,278,483	0	A	T	6.0%	198.7 / 2.8	66	intergenic (‑310/+253)	PP_5465/PP_1117	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/36);  new base T (2/2);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

TTGCGCTATGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGCCATGGA  >  NC_002947/1278339‑1278629                                                                                                                                                  |                                                                                                                                                   ttGCGCTATGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGt                                                                                                                                                 <  2:96332/148‑1 (MQ=255)     gcTATGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCtt                                                                                                                                            <  7:40893/149‑1 (MQ=255)     gcTATGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCtt                                                                                                                                            >  2:306932/1‑149 (MQ=255)      cTATGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTa                                                                                                                                           >  4:168483/1‑149 (MQ=255)         tGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATgg                                                                                                                                        <  1:306932/149‑1 (MQ=255)         tGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATg                                                                                                                                         <  3:268284/148‑1 (MQ=255)             cTACGGACAATGAAGAGCAAGAGCAGAAGCTTGAGTCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAg                                                                                                                                     >  1:46933/1‑149 (MQ=255)              tACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACTTTGTCTCTTac                                                                                                                                          >  8:107620/1‑141 (MQ=255)              tACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACTTTGTCTCTTac                                                                                                                                          <  7:107620/142‑2 (MQ=255)                cGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTggg                                                                                                                                 >  6:81064/1‑149 (MQ=255)                 ggACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACTTTGTCTCTTa                                                                                                                                           <  4:278186/138‑1 (MQ=255)                 ggACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACTTTGTCTCTTa                                                                                                                                           >  3:278186/1‑138 (MQ=255)                 ggACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGt                                                                                                                                >  4:152735/1‑149 (MQ=255)                 ggACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGt                                                                                                                                <  3:16946/149‑1 (MQ=255)                          agagcaagagcaGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTc                                                                                                                        <  5:48803/148‑1 (MQ=255)                           gagcaagagcaGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTc                                                                                                                        >  2:281616/1‑147 (MQ=255)                           gagcaagagcaGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCaa                                                                                                                      <  6:258300/149‑1 (MQ=255)                             gcaagagcaGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGCTGTCAAGt                                                                                                                    <  1:196832/149‑1 (MQ=255)                                  agcaGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGata                                                                                                                >  8:1732/1‑148 (MQ=255)                                     aGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCt                                                                                                             >  4:118371/1‑148 (MQ=255)                                        aGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGAt                                                                                                          <  3:168483/148‑1 (MQ=255)                                              a‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGa                                                                                                    <  5:245606/148‑1 (MQ=255)                                              a‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGa                                                                                                    <  5:241307/148‑1 (MQ=255)                                                tCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACTGTACGTCAGAAACGGGAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGAt                                                                                                   <  5:275022/148‑1 (MQ=255)                                                tCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCTCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGAt                                                                                                   <  7:140355/148‑1 (MQ=255)                                                    tgtAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGc                                                                                                <  2:37932/147‑1 (MQ=255)                                                      tAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTg                                                                                                                   <  6:177326/126‑1 (MQ=255)                                                      tAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTg                                                                                                                   >  5:177326/1‑126 (MQ=255)                                                      tAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACa                                                                                            <  3:152735/149‑1 (MQ=255)                                                          attaattaTTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAg                                                                                        <  2:46933/149‑1 (MQ=255)                                                          attaattaTTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAg                                                                                        >  7:192810/1‑149 (MQ=255)                                                           ttaattaTTATAAATCAACATCCTGATAGTCAAGTGTTGACTGACGGTACGTAAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGAGGGTTAGTTGACAAGTGATATCTGATAGCCGATCGATACATGAgg                                                                                       >  1:223362/1‑149 (MQ=255)                                                            taattaTTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGAt                                                                                                          >  7:139899/1‑129 (MQ=255)                                                            taattaTTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGAt                                                                                                          <  8:139899/129‑1 (MQ=255)                                                              attattATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTg                                                                                    <  5:81064/149‑1 (MQ=255)                                                                   tataAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACAtt                                                                               >  5:89129/1‑149 (MQ=255)                                                                   tataAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACAtt                                                                               >  3:88693/1‑149 (MQ=255)                                                                    ataAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACAtt                                                                               <  6:89129/148‑1 (MQ=255)                                                                     taAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGatat                                                                                                               <  5:206162/115‑1 (MQ=255)                                                                     taAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGatat                                                                                                               >  6:206162/1‑115 (MQ=255)                                                                           aaCATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGtt                                                                                                                           <  5:284177/97‑1 (MQ=255)                                                                           aaCATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGtt                                                                                                                           >  6:284177/1‑97 (MQ=255)                                                                           aaCATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACtt                                                                       <  1:281616/149‑1 (MQ=255)                                                                              aTCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCg                                                                    <  7:1732/149‑1 (MQ=255)                                                                                 cTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGtt                                                                  <  4:88693/148‑1 (MQ=255)                                                                                        tGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAAc                                                                         <  8:110095/134‑1 (MQ=255)                                                                                        tGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAAc                                                                         >  7:110095/1‑134 (MQ=255)                                                                                         gAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCa                                                         >  3:114305/1‑149 (MQ=255)                                                                                         gAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCa                                                         >  4:68998/1‑149 (MQ=255)                                                                                                  ccTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATaga                                                 <  8:137208/148‑1 (MQ=255)                                                                                                      aCGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGa                                                                                                           <  6:194289/86‑1 (MQ=255)                                                                                                      aCGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGtt                                              <  3:68998/147‑1 (MQ=255)                                                                                                          tACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCtgtg                                         >  1:227365/1‑148 (MQ=255)                                                                                                                aGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACTGTGCATTGATAGAGTTCTGTGCAGaaa                                   >  4:186563/1‑148 (MQ=255)                                                                                                                   aaCCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATcc                                <  7:117887/148‑1 (MQ=255)                                                                                                                             ccAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGaaa                     >  7:171396/1‑149 (MQ=255)                                                                                                                                   ggAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGc               <  4:114305/149‑1 (MQ=255)                                                                                                                                      gCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTc             >  1:120164/1‑148 (MQ=255)                                                                                                                                       cGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCaa           <  3:118371/149‑1 (MQ=255)                                                                                                                                         tAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAgg         <  8:171396/149‑1 (MQ=255)                                                                                                                                          aaGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGc        >  6:267235/1‑149 (MQ=255)                                                                                                                                             agaCAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGCCAt     <  8:192810/149‑1 (MQ=255)                                                                                                                                              gaCAAAGTCTCTTATTGGAGTGGGTTAGTTGTCAAGTGATATCTGATATCCGATGCATACATGAGGTTGACATTGTCAACTTGCGTTGACCGTTCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGCCAt     <  3:172341/148‑1 (MQ=255)                                                                                                                                               aCAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGCCATg    >  7:112327/1‑148 (MQ=255)                                                                                                                                               aCAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGCCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGAAGAAATCCGTCACTTGAAATGTGGCTCAATGACATga   >  4:172341/1‑148 (MQ=255)                                                                                                                                                cAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGCCATGGa  <  5:267235/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TTGCGCTATGTCCTACGGACAATGAAGAGCAAGAGCAGAAGCTTGA‑TCTGTGTAAAATTAATTATTATAAATCAACATCCTGATAGTGAAGTGTTGCCTGACGGTACGTCAGAAACCGCAATTCCAGTGGGAGCGTAAGAGACAAAGTCTCTTATGGGAGTGGGTTAGTTGTCAAGTGATATCTGATAGCCGATGGCTACATGAGGTTGACATTGTCAACTTGCGTTGACCGTGCATTGATAGAGTTCTGTGCAGAAATCCGTCACTTGAAATGTGGCTCAAGGCCATGGA  >  NC_002947/1278339‑1278629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑