Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,306,911	G→T	8.0%	A236D (GCT→GAT)	livK ←	branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,306,911	0	G	T	8.0%	189.5 / 4.1	61	A236D (GCT→GAT)	livK	branched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base T (3/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTCTTGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTT  >  NC_002947/1306767‑1307056                                                                                                                                                  |                                                                                                                                                  aTCTTCTTGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTg                                                                                                                                               >  2:145034/1‑149 (MQ=255)      cttGTTCGCCGGGTCCGCGTCTAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTg                                                                                                                                               <  7:321745/144‑1 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTgg                                                                                                                                              <  3:211101/145‑1 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTg                                                                                                                                               <  5:68568/144‑1 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTg                                                                                                                                               <  2:282314/144‑1 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGgcgc                                                                                                                                           <  5:166562/148‑1 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGgcg                                                                                                                                            >  6:87438/1‑147 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGgc                                                                                                                                             <  3:180618/146‑1 (MQ=255)      cttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGgc                                                                                                                                             <  7:190687/146‑1 (MQ=255)           tCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCATGAGCCTGGCGCAGGAt                                                                                                                                      >  6:228348/1‑148 (MQ=255)                   tCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAg                                                                                                                             >  8:178308/1‑149 (MQ=255)                            aaCGACTTCGGCAGGGTGACCAGCTGGCCTTCGGAGGCGTTCTGAGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGggtg                                                                                                                    <  8:79536/149‑1 (MQ=255)                            aaCGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGggtg                                                                                                                    >  8:15914/1‑149 (MQ=255)                              cGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTggg                                                                                                                      >  7:58030/1‑145 (MQ=255)                              cGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTggg                                                                                                                      <  8:58030/145‑1 (MQ=255)                               gACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTa                                                                                                                 <  5:184126/149‑1 (MQ=255)                                  ttCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGATCCTGCGCCAGGCTCAgg                                                                                                                                  >  5:328842/1‑129 (MQ=255)                                  ttCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGATCCTGCGCCAGGCTCAgg                                                                                                                                  <  6:328842/129‑1 (MQ=255)                                  ttCGGCAGGGCGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAgcc                                                                                                              >  5:150444/1‑149 (MQ=255)                                        aGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGccgccg                                                                                                          >  6:302761/1‑147 (MQ=255)                                            tGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCgtag                                                                                                       >  7:25757/1‑146 (MQ=255)                                            tGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGtagtag                                                                                                    <  5:228348/149‑1 (MQ=255)                                              aCCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGa                                                                                                   <  1:145034/148‑1 (MQ=255)                                                    agGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCGGTAGTAGACGAAGTc                                                                                            <  7:178308/149‑1 (MQ=255)                                                      gCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGa                                                                                          <  4:71686/149‑1 (MQ=255)                                                       ccTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGAc                                                                                         <  7:15914/149‑1 (MQ=255)                                                       ccTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGAc                                                                                         <  5:302761/149‑1 (MQ=255)                                                            ggAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgttgt                                                                                    >  7:44580/1‑149 (MQ=255)                                                            ggAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgttg                                                                                     <  8:25757/148‑1 (MQ=255)                                                              aGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                 >  1:163683/1‑134 (MQ=255)                                                              aGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                 <  2:163683/134‑1 (MQ=255)                                                                   ttCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCtt                                                                             <  8:44580/149‑1 (MQ=255)                                                                        ggCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCGAGCTCTGggtgg                                                                                                                   <  3:150543/106‑1 (MQ=255)                                                                        ggCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGggtgg                                                                                                                   >  4:150543/1‑106 (MQ=255)                                                                                 cGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGGTCTGGGTGGTAGCCGCCGTAGTTGACGAAGTCGACGTGTTTGTGATTTAGCTTCTGgatg                                                                >  1:258573/1‑149 (MQ=255)                                                                                        gAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgagga                                                        <  5:87438/149‑1 (MQ=255)                                                                                        gAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgag                                                          >  4:293057/1‑147 (MQ=255)                                                                                          gTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgag                                                          >  1:341551/1‑145 (MQ=255)                                                                                          gTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgag                                                          <  2:341551/145‑1 (MQ=255)                                                                                           tCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGATCCTGGCGCAGCATCAGGCCAAGCTCTGGGTGGTAGCCGCCTTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGGTTCTGGATGATCgaggat                                                       >  5:220673/1‑146 (MQ=255)                                                                                             gTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGt                                                   >  6:145486/1‑149 (MQ=255)                                                                                              ttACCCACGCCTTCCGGAACCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTc                                                  >  3:330994/1‑149 (MQ=255)                                                                                                aCCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCt                                                >  1:166657/1‑149 (MQ=255)                                                                                                  ccACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTg                                              >  8:289523/1‑149 (MQ=255)                                                                                                     cGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCa                                           >  8:98978/1‑149 (MQ=255)                                                                                                          tCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCcgc                                      >  3:60709/1‑149 (MQ=255)                                                                                                               aCCCATGAACTTGGCTTTCA‑GTCCTTTTTCCTGAGCGTTGCGCAGGTTCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCa                                 <  4:330994/149‑1 (MQ=255)                                                                                                               aCCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGtt                                   >  3:192620/1‑147 (MQ=255)                                                                                                                 ccATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAgg                               >  7:146310/1‑149 (MQ=255)                                                                                                                         ttGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGCTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGaa                       >  7:265640/1‑149 (MQ=255)                                                                                                                                 cA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACg                   >  2:242336/1‑145 (MQ=255)                                                                                                                                 cA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATCATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACg                   <  1:242336/145‑1 (MQ=255)                                                                                                                                    ggCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAAcc             <  4:60709/149‑1 (MQ=255)                                                                                                                                            tCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGcc     >  3:98612/1‑149 (MQ=255)                                                                                                                                             ccTGATCCTGCGCCAGGCTCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGCGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGc      <  8:157862/147‑1 (MQ=255)                                                                                                                                             ccTGATCCTGCGCCAGGCTCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGc      >  7:157862/1‑147 (MQ=255)                                                                                                                                             ccTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt    >  7:224704/1‑149 (MQ=255)                                                                                                                                             ccTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt    >  8:100860/1‑149 (MQ=255)                                                                                                                                              cTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCtt   <  7:98978/149‑1 (MQ=255)                                                                                                                                              cTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt    <  7:100860/148‑1 (MQ=255)                                                                                                                                                 aGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCttt  <  8:224704/147‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ATCTTCTTGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTT  >  NC_002947/1306767‑1307056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑