Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,306,923 A→C 7.6% I232S (ATC→AGC)  livK ← branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,306,9230AC 7.6% 157.3 / 4.6 53I232S (ATC→AGC) livKbranched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (27/21);  new base C (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGCTT  >  NC_002947/1306777‑1307060
                                                                                                                                                   |                                                                                                                                         
tCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCATGAGCCTGGCGCAGGAt                                                                                                                                          >  6:228348/1‑148 (MQ=255)
        tCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAg                                                                                                                                 >  8:178308/1‑149 (MQ=255)
                 aaCGACTTCGGCAGGGTGACCAGCTGGCCTTCGGAGGCGTTCTGAGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGggtg                                                                                                                        <  8:79536/149‑1 (MQ=255)
                 aaCGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGggtg                                                                                                                        >  8:15914/1‑149 (MQ=255)
                   cGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTggg                                                                                                                          <  8:58030/145‑1 (MQ=255)
                   cGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTggg                                                                                                                          >  7:58030/1‑145 (MQ=255)
                    gACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTa                                                                                                                     <  5:184126/149‑1 (MQ=255)
                       ttCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGATCCTGCGCCAGGCTCAgg                                                                                                                                      >  5:328842/1‑129 (MQ=255)
                       ttCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGATCCTGCGCCAGGCTCAgg                                                                                                                                      <  6:328842/129‑1 (MQ=255)
                       ttCGGCAGGGCGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAgcc                                                                                                                  >  5:150444/1‑149 (MQ=255)
                             aGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGccgccg                                                                                                              >  6:302761/1‑147 (MQ=255)
                                 tGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCgtag                                                                                                           >  7:25757/1‑146 (MQ=255)
                                 tGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGtagtag                                                                                                        <  5:228348/149‑1 (MQ=255)
                                   aCCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGa                                                                                                       <  1:145034/148‑1 (MQ=255)
                                         agGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCGGTAGTAGACGAAGTc                                                                                                <  7:178308/149‑1 (MQ=255)
                                           gCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGa                                                                                              <  4:71686/149‑1 (MQ=255)
                                            ccTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGAc                                                                                             <  7:15914/149‑1 (MQ=255)
                                            ccTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGAc                                                                                             <  5:302761/149‑1 (MQ=255)
                                                 ggAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgttgt                                                                                        >  7:44580/1‑149 (MQ=255)
                                                 ggAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgttg                                                                                         <  8:25757/148‑1 (MQ=255)
                                                   aGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                     <  2:163683/134‑1 (MQ=255)
                                                   aGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                     >  1:163683/1‑134 (MQ=255)
                                                        ttCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCtt                                                                                 <  8:44580/149‑1 (MQ=255)
                                                             ggCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCGAGCTCTGggtgg                                                                                                                       <  3:150543/106‑1 (MQ=255)
                                                             ggCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGggtgg                                                                                                                       >  4:150543/1‑106 (MQ=255)
                                                                      cGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGGTCTGGGTGGTAGCCGCCGTAGTTGACGAAGTCGACGTGTTTGTGATTTAGCTTCTGgatg                                                                    >  1:258573/1‑149 (MQ=255)
                                                                             gAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgagga                                                            <  5:87438/149‑1 (MQ=255)
                                                                             gAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgag                                                              >  4:293057/1‑147 (MQ=255)
                                                                               gTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgag                                                              >  1:341551/1‑145 (MQ=255)
                                                                               gTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCgag                                                              <  2:341551/145‑1 (MQ=255)
                                                                                tCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGATCCTGGCGCAGCATCAGGCCAAGCTCTGGGTGGTAGCCGCCTTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGGTTCTGGATGATCgaggat                                                           >  5:220673/1‑146 (MQ=255)
                                                                                  gTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGt                                                       >  6:145486/1‑149 (MQ=255)
                                                                                   ttACCCACGCCTTCCGGAACCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTc                                                      >  3:330994/1‑149 (MQ=255)
                                                                                     aCCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCt                                                    >  1:166657/1‑149 (MQ=255)
                                                                                       ccACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTg                                                  >  8:289523/1‑149 (MQ=255)
                                                                                          cGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCa                                               >  8:98978/1‑149 (MQ=255)
                                                                                               tCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCcgc                                          >  3:60709/1‑149 (MQ=255)
                                                                                                    aCCCATGAACTTGGCTTTCA‑GTCCTTTTTCCTGAGCGTTGCGCAGGTTCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCa                                     <  4:330994/149‑1 (MQ=255)
                                                                                                    aCCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGtt                                       >  3:192620/1‑147 (MQ=255)
                                                                                                      ccATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAgg                                   >  7:146310/1‑149 (MQ=255)
                                                                                                              ttGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGCTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGaa                           >  7:265640/1‑149 (MQ=255)
                                                                                                                      cA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACg                       >  2:242336/1‑145 (MQ=255)
                                                                                                                      cA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATCATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACg                       <  1:242336/145‑1 (MQ=255)
                                                                                                                         ggCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAAcc                 <  4:60709/149‑1 (MQ=255)
                                                                                                                                 tCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGcc         >  3:98612/1‑149 (MQ=255)
                                                                                                                                  ccTGATCCTGCGCCAGGCTCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGCGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGc          <  8:157862/147‑1 (MQ=255)
                                                                                                                                  ccTGATCCTGCGCCAGGCTCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGc          >  7:157862/1‑147 (MQ=255)
                                                                                                                                  ccTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt        >  8:100860/1‑149 (MQ=255)
                                                                                                                                  ccTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt        >  7:224704/1‑149 (MQ=255)
                                                                                                                                   cTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCtt       <  7:98978/149‑1 (MQ=255)
                                                                                                                                   cTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt        <  7:100860/148‑1 (MQ=255)
                                                                                                                                      aGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCttt      <  8:224704/147‑1 (MQ=255)
                                                                                                                                        ccTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGCtt  <  4:98612/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
TCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCA‑GGCCTTTTTCCTGAGCCTGGCGCAGGATCAGGCCCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTTCTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGCTT  >  NC_002947/1306777‑1307060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑