Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,307,449 T→G 8.6% N57H (AAC→CAC)  livK ← branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,307,4490TG 8.6% 194.7 / 4.6 67N57H (AAC→CAC) livKbranched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base G (3/3);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTGCGGCG  >  NC_002947/1307303‑1307594
                                                                                                                                                  |                                                                                                                                                 
ggTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGACGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTg                                                                                                                                                  <  6:38305/148‑1 (MQ=255)
 gTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                                <  8:116294/149‑1 (MQ=255)
  tggagctggagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGata                                                                                                                                               <  8:190017/149‑1 (MQ=255)
    gagctggagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATc                                                                                                                                             <  4:144813/149‑1 (MQ=255)
     agctggagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATcc                                                                                                                                            >  2:60284/1‑149 (MQ=255)
          gagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGAt                                                                                                                                       <  8:241298/149‑1 (MQ=255)
          gagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGAt                                                                                                                                       <  8:194016/149‑1 (MQ=255)
             cacaGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                                <  8:287867/137‑1 (MQ=255)
             cacaGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                                >  7:287867/1‑137 (MQ=255)
             cacaGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgc                                                                                                                                    >  4:126157/1‑149 (MQ=255)
                            aCGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCtt                                                                                                                                          <  6:279979/128‑1 (MQ=255)
                            aCGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCtt                                                                                                                                          >  5:279979/1‑128 (MQ=255)
                            aCGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGAt                                                                                                                     >  6:43485/1‑149 (MQ=255)
                             cGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGAt                                                                                                                     >  4:62880/1‑148 (MQ=255)
                                    gACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCa                                                                                                             >  7:312044/1‑149 (MQ=255)
                                    gACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCCCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTg                                                                                                               <  1:281259/147‑1 (MQ=255)
                                          gtcgtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGc                                                                                                       >  5:48361/1‑149 (MQ=255)
                                                  ccACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTgg                                                                                               <  3:126157/149‑1 (MQ=255)
                                                  ccACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTgg                                                                                               >  4:253491/1‑149 (MQ=255)
                                                   cACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTggg                                                                                              >  7:109205/1‑149 (MQ=255)
                                                     cTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGt                                                                                             >  5:175518/1‑148 (MQ=255)
                                                         gttgGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACgg                                                                                        >  1:161849/1‑149 (MQ=255)
                                                          tttgCGACTGCCACGTCCTGTTGAGGGTCGAAGGCGTCGTCGTATTGCTTGGCTTCGAGGATCTTGCCATCCTCGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCGGATGATCTGCATGTCGCCGTACTGGGTCActg                                                                                        <  4:242490/145‑3 (MQ=255)
                                                            ggCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTTCATCTCGCCGTACTGGGTCACGGGAc                                                                                     <  7:52032/149‑1 (MQ=255)
                                                            ggCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGa                                                                                      <  6:339800/148‑1 (MQ=255)
                                                                  tGCAACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATACACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCTCCGTACTGGGTCACGGGACCAGTCt                                                                               >  7:118976/1‑149 (MQ=255)
                                                                    ccACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTg                                                                             >  8:103000/1‑149 (MQ=255)
                                                                        ggCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGAc                                                                         <  8:312044/149‑1 (MQ=255)
                                                                         gCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTTGCCGTACTgg                                                                                               <  6:316716/126‑1 (MQ=255)
                                                                         gCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTTGCCGTACTgg                                                                                               >  5:316716/1‑126 (MQ=255)
                                                                          ccTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCg                                                                       >  2:320241/1‑149 (MQ=255)
                                                                                 aGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGAAATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGTGTCACGGGACCCGTCTTGGGACCGCCGAtac                                                                >  3:264764/1‑147 (MQ=255)
                                                                                           gCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                                      <  1:320241/149‑1 (MQ=255)
                                                                                           gCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                                      <  1:60284/149‑1 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGa                                                       >  4:262753/1‑142 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGa                                                       <  3:262753/142‑1 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATc                                                <  4:161069/149‑1 (MQ=255)
                                                                                                   gtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcgg                                              <  3:62880/149‑1 (MQ=255)
                                                                                                     aTTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcg                                            >  4:240952/1‑149 (MQ=255)
                                                                                                     aTTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcg                                            >  2:178914/1‑149 (MQ=255)
                                                                                                         cTTGGATTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTTATGGCTTGCTTGGCGCCGATTAACTGCATGTAGACGTACTGTGTCACGGGAACCGGCATGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCa                                         >  6:316093/1‑148 (MQ=255)
                                                                                                          ttGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCaaa                                       <  5:43485/149‑1 (MQ=255)
                                                                                                               ttCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAt                                  >  1:102294/1‑149 (MQ=255)
                                                                                                               ttCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAt                                  >  5:13393/1‑149 (MQ=255)
                                                                                                                  gAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggc                               >  4:326284/1‑149 (MQ=255)
                                                                                                                  gAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggc                               >  5:316538/1‑149 (MQ=255)
                                                                                                                    gCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctg                             <  7:103000/149‑1 (MQ=255)
                                                                                                                       tCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGa                                                                          <  8:162693/101‑1 (MQ=255)
                                                                                                                       tCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGa                                                                          >  7:162693/1‑101 (MQ=255)
                                                                                                                         ttGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTAc                        >  3:127257/1‑149 (MQ=255)
                                                                                                                            ccATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTAcccc                     <  3:253491/149‑1 (MQ=255)
                                                                                                                            ataTCAACGCCGCGGGCGGCGTGGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTAcccc                     >  6:308541/3‑149 (MQ=255)
                                                                                                                             tttcaaCGCCGCGGGCGGCGTGGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCg                    >  2:62610/6‑149 (MQ=255)
                                                                                                                             cATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCg                    >  6:295322/1‑149 (MQ=255)
                                                                                                                              aTCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCgg                   >  3:277888/1‑149 (MQ=255)
                                                                                                                               tcaaCGCCGCGGGCGGCGTGGATATCCTTGATGGCTTGCTTGGCGCCGATGATCTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc                  <  5:308541/146‑1 (MQ=255)
                                                                                                                               tCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc                  <  2:161849/149‑1 (MQ=255)
                                                                                                                               tCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc                  >  1:23231/1‑149 (MQ=255)
                                                                                                                               tCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc                  <  4:277888/149‑1 (MQ=255)
                                                                                                                                 aaCGCCGCGGGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc                  >  6:230474/2‑147 (MQ=255)
                                                                                                                                  aCGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAg               >  3:114367/1‑149 (MQ=255)
                                                                                                                                       gcccgcGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAACCGAATGGCTGGCTACCCCGGCCAGTACCa          >  2:280549/1‑149 (MQ=255)
                                                                                                                                        cccgcGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCAt         <  2:102294/149‑1 (MQ=255)
                                                                                                                                            cggcgTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTgcg     <  5:230474/149‑1 (MQ=255)
                                                                                                                                             ggcgTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTgcgg    <  2:196384/149‑1 (MQ=255)
                                                                                                                                              gcgTTGATATCCTTGATGGCTTGCTTGGCCCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTgcggc   <  5:307627/149‑1 (MQ=255)
                                                                                                                                                gTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTgcggcg  >  4:81617/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTGCGGCG  >  NC_002947/1307303‑1307594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑