Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,355,600 C→T 5.9% A44V (GCC→GTC)  PP_1180 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,355,6000CT 5.9% 207.5 / 2.0 68A44V (GCC→GTC) PP_1180hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTTGTGTGTGA  >  NC_002947/1355455‑1355746
                                                                                                                                                 |                                                                                                                                                  
tCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGc                                                                                                                                                    >  2:31132/1‑146 (MQ=255)
  gcgGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCa                                                                                                                                                  <  1:31132/146‑1 (MQ=255)
     gggATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg                                                                                                                                            <  2:192559/149‑1 (MQ=255)
           cgcAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGc                                                                                                                                      <  8:241032/149‑1 (MQ=255)
            gcAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCa                                                                                                                                     <  6:94474/149‑1 (MQ=255)
                tgcatgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGCGCCCAAAAACTGTTGCTGATGGGTGTCAGGCCTGGCCTGCAacac                                                                                                                                 >  4:228678/1‑149 (MQ=255)
                tgcatgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGTCAGGCCTGGCCTGCAacac                                                                                                                                 >  4:330958/1‑149 (MQ=255)
                tgcatgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAacac                                                                                                                                 >  8:113217/1‑149 (MQ=255)
                  catgcACATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGTCTCAGTAATAAGGGGCAATGGCTTGCCGCTCCCTGGCAGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACAcc                                                                                                                                <  8:262810/148‑1 (MQ=255)
                       gcATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGcc                                                                                                                          >  7:136388/1‑149 (MQ=255)
                            cgcccgcGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAac                                                                                                                                   >  1:33533/1‑135 (MQ=255)
                            cgcccgcGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAac                                                                                                                                   <  2:33533/135‑1 (MQ=255)
                                  cgcgGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACAccc                                                                                                                               <  4:21742/133‑1 (MQ=255)
                                  cgcgGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACAccc                                                                                                                               >  3:21742/1‑133 (MQ=255)
                                  cgcgGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGcc                                                                                                               <  7:113217/149‑1 (MQ=255)
                                   gcgGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGccc                                                                                                              <  5:334603/149‑1 (MQ=255)
                                       gCGTCCCGACGTCTGGACAGACTCACGAATAAGGGGCAATGGCATCCCGCTCCCTGGCAGAAGGCAACGGTTCTGTGCCGAGCCCAAAAACTGTTGCTGATGGGAGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGaa                                                                                                          <  3:287555/149‑1 (MQ=255)
                                                     ggACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCgg                                                                                            >  3:117053/1‑149 (MQ=255)
                                                      gACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGt                                                                                           >  7:120340/1‑149 (MQ=255)
                                                       aCAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTc                                                                                          <  8:120340/149‑1 (MQ=255)
                                                             tCAGGAATAAGGGGCATTGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTcc                                                                                    >  6:208271/1‑149 (MQ=255)
                                                             tCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCt                                                                                                             <  5:15394/124‑1 (MQ=255)
                                                             tCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCt                                                                                                             >  6:15394/1‑124 (MQ=255)
                                                             tCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTcc                                                                                    >  1:41128/1‑149 (MQ=255)
                                                                  aaTAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAg                                                                                >  4:111878/1‑148 (MQ=255)
                                                                    tAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGACCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAg                                                                             >  1:131560/1‑149 (MQ=255)
                                                                           cAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTg                                                                      <  1:96342/149‑1 (MQ=255)
                                                                           cAATGGCTTGCCGCTCCCTCGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGATGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATCCCCGAACAACGCCCTGAAAGGGCTTGACTCGCTCGCATCCGCAGGAGCGGttag                                                                      >  2:96342/1‑147 (MQ=255)
                                                                                gCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGgctg                                                                  <  3:87574/148‑1 (MQ=255)
                                                                                 cTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCGGCAACACCCATGCCAGAACAACGCGCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGgctgct                                                                <  5:242346/149‑1 (MQ=255)
                                                                                     ccGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGaaa                                                            >  6:142904/1‑149 (MQ=255)
                                                                                         tCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCAc                                                        >  1:92719/1‑149 (MQ=255)
                                                                                           ccTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGaacaa                                                                                                                   >  5:165899/1‑88 (MQ=255)
                                                                                           ccTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGaacaa                                                                                                                   <  6:165899/88‑1 (MQ=255)
                                                                                           ccTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCAc                                                        <  8:47454/147‑1 (MQ=255)
                                                                                                 cGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGt                                                 >  2:146079/1‑148 (MQ=255)
                                                                                                  gAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTc                                               >  3:40048/1‑149 (MQ=255)
                                                                                                      tCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGgctg                                                                  <  8:40134/126‑1 (MQ=255)
                                                                                                      tCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGgctg                                                                  >  7:40134/1‑126 (MQ=255)
                                                                                                      tCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCAcc                                           >  3:140979/1‑149 (MQ=255)
                                                                                                       cAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTcccccc                                          >  4:191621/1‑149 (MQ=255)
                                                                                                        aaaGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTg                                                                      <  4:146379/120‑1 (MQ=255)
                                                                                                        aaaGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTg                                                                      >  3:146379/1‑120 (MQ=255)
                                                                                                        aaaGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGa                                                              >  1:173244/1‑128 (MQ=255)
                                                                                                        aaaGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGa                                                              <  2:173244/128‑1 (MQ=255)
                                                                                                        aaaGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCg                                         >  4:1092/1‑149 (MQ=255)
                                                                                                        aaaGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCg                                         >  2:209897/1‑149 (MQ=255)
                                                                                                            gTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGaaa                                     <  3:35497/149‑1 (MQ=255)
                                                                                                             ttCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGaaaa                                    >  5:73239/1‑149 (MQ=255)
                                                                                                                  ggCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGc                                >  6:118804/1‑148 (MQ=255)
                                                                                                                  ggCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGc                                <  5:118804/148‑1 (MQ=255)
                                                                                                                  ggCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCt                               >  3:181292/1‑149 (MQ=255)
                                                                                                                        gCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCt                         >  6:245485/1‑149 (MQ=255)
                                                                                                                          ccAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTg                       >  2:84225/1‑149 (MQ=255)
                                                                                                                           cAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGAt                           <  2:334754/144‑1 (MQ=255)
                                                                                                                           cAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGAt                           >  1:334754/1‑144 (MQ=255)
                                                                                                                           cAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTg                       <  3:157976/148‑1 (MQ=255)
                                                                                                                           cAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTg                       >  4:157976/1‑148 (MQ=255)
                                                                                                                            aaaaaCTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCg                     >  1:327870/1‑149 (MQ=255)
                                                                                                                             aaaaCTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCgg                    <  3:1092/149‑1 (MQ=255)
                                                                                                                                aCTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGGAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGtt                 <  5:150866/149‑1 (MQ=255)
                                                                                                                                       cTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACtttt          <  8:136388/149‑1 (MQ=255)
                                                                                                                                          aTGGGTGTCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGGAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTtgt        <  3:330958/148‑1 (MQ=255)
                                                                                                                                          aTGGGTGTCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTtgt        <  3:228678/148‑1 (MQ=255)
                                                                                                                                          aTGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTtgtg       <  3:111878/149‑1 (MQ=255)
                                                                                                                                          aTGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTtgt        <  5:245485/148‑1 (MQ=255)
                                                                                                                                          aTGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTtgt        <  5:208271/148‑1 (MQ=255)
                                                                                                                                           tGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTtgtgt      <  2:41128/149‑1 (MQ=255)
                                                                                                                                               tGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAGGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTTGTGTGTGa  <  4:117053/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCATGCCCGAACAACGCCCTGAAAGGGCTTGCCTCGGTCGCCTCCGCAGGAGCGGTTTGGCTGCTGAAATCACCTGAAGTTCCACCCGGAAAATCGCTTGATCTTGCGGGTTGACTTTTGTGTGTGA  >  NC_002947/1355455‑1355746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑